共查询到20条相似文献,搜索用时 31 毫秒
1.
Lan XY Shu JH Chen H Pan CY Lei CZ Wang X Liu SQ Zhang YB 《Molecular biology reports》2009,36(6):1371-1374
POU1F1 is a positive regulator for prolactin (PRL) whose metabolites may directly or indirectly affect some aspects of the
hair growth cycle, therefore, POU1F1 gene is an important candidate gene for cashmere traits selection through marker-assisted selection (MAS). Hence, in this
study, the PCR-RFLP method was applied to detect a T>C transition determining a PstI polymorphism at the 3′UTR of POU1F1 locus and evaluate its associations with cashmere traits in 847 Inner Mongolia White Cashmere goats. In the analyzed population,
the allelic frequencies for the T and C alleles are 0.959 and 0.041, respectively and the genotypic frequencies are in Hardy-Weinberg
equilibrium (P > 0.05). Moreover, significant statistical relationships between the PstI polymorphism of POU1F1 gene and goat cashmere yields were found (*P < 0.05). When compared with TC genotype, TT genotype was associated with superior cashmere yields in 2, 4, and 5 years old
individuals, as well as with average cashmere yield. Hence, TT genotype is suggested to be a molecular marker for senior cashmere
yield.
X. Y. Lan and J. H. Shu have contributed equally to this article. 相似文献
2.
In this study, genetic variability at codon 42 within prion protein (PRNP) gene and its associations with production traits were investigated in 2002 goats from four Chinese domestic breeds. The
frequencies of allele “A” ranged from 0.353 to 0.562 in analyzed goat breeds with Hardy–Weinberg equilibrium (P > 0.05) except Xinong Sannen (XNSN) dairy breed. The establishment of relationships between different genotypes and growth
traits was performed in Inner Mongolia white Cashmere (IMWC) breed and revealed an association of the polymorphism with body
weight at 7-year-old goats (P = 0.033). The individuals with genotype GG showed heavier body weight than those with genotype AA. Moreover, association
analysis detected two significant associations between different genotypes and cashmere yield and fiber length in IMWC breed
(P = 0.009, P = 0.048, respectively). In addition, three significant associations of different genotypes with density of milk (a.m. and
p.m.), solids-not-fat of milk (P = 0.013, P = 0.009 and P = 0.002), respectively, were found in XNSN breed. Genotype GG had better milk quality than others. These findings suggested
that the polymorphism of codon 42 within PRNP was a useful DNA marker for eliminating or selecting excellent individuals in relation to production traits in marker-assist
selection breeding of goat. 相似文献
3.
Zhuanjian Li Zhongqi Chen Xianyong Lan Liang Ma Yujiao Qu Yanli Liu Mijie Li Ping Wang Fei Li Hong Chen 《Molecular biology reports》2010,37(1):563-569
Weaver gene plays an essential physiological role in the function of many organs, including brain, heart, kidney and endocrine cells,
and also in the regulation of insulin secretion by glucose and/or neurotransmitters. Thus, weaver gene is an important potential candidate gene effecting on performance traits. The objective of this study was to detect
the genetic variation of five loci within weaver gene by PCR-SSCP, DNA sequencing and forced PCR-RFLP methods in 1,019 Chinese indigenous goats. Two novel coding SNPs (XM_598993:m.864G>A;
XM_598993:m.1224T>A) locating on P3 and P4 loci were identified and detected by MluI and AsuII forced PCR-RFLP, respectively. In the MluI analysis, the frequencies of goat MluI-A allele in the analyzed populations were 0.226, 0.248, 0.096 and 0.088 for XNSN, GZ, SBWC and XJWC, respectively. Genotypic
frequencies were found to be significantly different in four breeds (χ2 = 75.842, df = 6, P < 0.001); In the AsuII analysis, the frequencies of goat AsuII-A allele in the analyzed populations were 0.584, 0.441, 0.073 and 0.063 for XNSN, GZ, SBWC and XJWC, respectively. Genotypic
frequencies were found to be significantly different among four breeds (χ2 = 399.464, df = 6, P < 0.001). The frequencies of allele MluI-A and AsuII-A in XNSN and GZ populations were significantly higher than those of SBWC and XJWC goats. Association analysis with adjusted
milk yield in the XNSN breed indicated that the animals with AsuII-AA genotype owned significantly higher adjusted milk yield than the ones with AsuII-TT genotype in the second lactation (P < 0.05). The observation suggested that the allele “AsuII-A” had the positive effects on adjusted milk yield in the second lactation. 相似文献
4.
G. Ren H. Chen L. Z. Zhang X. Y. Lan T. B. Wei M. J. Li Y. J. Jing C. Z. Lei J. Q. Wang 《Molecular biology reports》2010,37(1):417-422
Heterozygous mutations in LHX4 are associated with combined pituitary hormone deficiency. In this study, the polymorphism of LHX4-HaeIII locus was revealed in 822 individuals from four Chinese cattle breeds. The PCR–RFLP analysis showed that there were three
genotypes: GG, GA, AA. The frequencies of genotype GG ranged from 0.6620 to 0.9789 in analyzed populations. The genotypic
frequencies of LHX4 locus in the four populations all agreed with Hardy–Weinberg equilibrium (P > 0.05). Distributions of genotypic frequencies of different breeds (QC, NY, JX, CH) at this locus were found to be significantly
different based on a χ
2 test (P < 0.001). The genetic diversity analysis revealed the JX cattle possessed intermediate genetic diversity, and the other three
Chinese cattle breeds belonged to poor genetic diversity. Correlation analysis with growth traits in the NY breed indicated
that: the animals with genotype GA had greater body weight than those with genotype GG (P < 0.05); the animals with GA genotype owned significantly longer body length than the ones with GG genotype (P < 0.05) at 18 and 24 months. 相似文献
5.
Polymorphisms of myostatin (MSTN) gene were investigated as a candidate marker for goat growth in 687 individuals by gene sequencing and polymerase chain
reaction restriction fragment length polymorphism methods. Three potential genotypes (AA, AB and BB) of 5 bp indel (1,256
TTTTA/−) in 5′UTR were detected in four breeds. The polymorphism (CC, CD and DD) of substitution (1,388 T/A) in exon 1 was
only segregating in Boer. Genotype AB resulted in significant increases in body weights at birth (BW0), 90 (BW90) and 300
(BW300) days of age, and birth body length compared to BB (P < 0.05). Whilst genotype CD contributed to heavier BW0 and BW90, and larger birth body height (BH) compared to CC (P < 0.05). Individuals either with AB or CD genotype also had greater values in other body sizes, although no significant differences
appeared (P > 0.05). When in combination, the combined genotype ABCD displayed positive impacts on better growth traits in BW0, BW90,
BW300, BH and BCG (P < 0.05). These results indicate that these markers in myostatin (MSTN) are associated with Boer growth and may be useful for marker assisted selection. 相似文献
6.
7.
Yanli Liu Xianyong Lan Yujiao Qu Zhuanjian Li Zhongqi Chen Chuzhao Lei Xingtang Fang Hong Chen 《Molecular biology reports》2011,38(1):539-544
Growth hormone-releasing hormone receptor (GHRHR) plays a critical role in growth hormone (GH) synthesis, release and regulation
of pituitary somatotroph expansion in vertebrates. The objective of this study was to investigate variations in goat GHRHR gene and their associations with growth traits in 668 dairy goats. The results showed four novel single nucleotide polymorphisms
(SNPs): NC_007302:g.5203C>T, 7307C>G, 9583G>A and 9668A>C. In detail, the novel SNP C>T in the 5203rd nucleotide identified
a missense mutation: CCC (Pro)>TCC (Phe) at position 116aa of the goat GHRHR (423aa). Besides, 9583G>A and 9668A>C polymorphism were in complete linkage disequilibrium. The genetic diversity analysis
revealed that the Guanzhong dairy goat possessed intermediate genetic diversity in P3 and P7 loci, and the Xinong Sannen dairy
goat belonged to poor genetic diversity in P4 locus. Significant associations between the genotypes of P3 locus and body length,
body height and chest circumference was observed in Guanzhong goat (P < 0.05). However, in Xinong saanen population, significant statistical difference was only found in body height and body
length (P < 0.05). In P4 and P7 loci, no significant associations were detected between any variant sites and body length, body height
and chest circumference, as well as for the milk traits (P > 0.05). These results strongly suggested that the goat GHRHR gene is a candidate gene that influences growth traits in dairy goat. 相似文献
8.
R. N. Ma C. J. Deng X. M. Zhang X. P. Yue X. Y. Lan H. Chen C. Z. Lei 《Molecular Biology》2010,44(4):536-540
The α-lactalbumin (α-LA) plays a key role in lactose synthesis in mammary glands of domestic animals. Mutations in the α-LA gene are associated with the milk traits in dairy cattle. In our study, a novel SNP: NO_X06366: g.875 C > T was detected in 708 dairy goat individuals—268 of the Xinong Saanen breed and 440 of Guanzhong breed, which revealed a synonymous
mutation in the exon 1 of α-LA gene. The Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) and sequencing techniques showed that
there were three genotypes: CC, CT and TT. Moreover, the χ2-test showed that the genotype frequencies of the two breeds were in good agreement with the Hardy-Weinberg equilibrium (P > 0.05). The relationship of the polymorphism of dairy goat α-LA gene with the milk trait and the body size trait was revealed. Individuals with the CC genotype were significantly smaller at chest circumference than those with CT (P < 0.05) in both breeds. But the milk trait and other body size traits of the two dairy goat breeds had no significant association
with genotypes studied. 相似文献
9.
As an essential repressor, the homeobox gene Hesx1/HESX1 is required within the anterior neural plate for normal forebrain development. Mutations within the Hesx1 gene have been associated with GH deficiency or combined pituitary hormone deficiency. We detected the polymorphism of Hesx1 gene by PCR-SSCP and DNA sequencing methods in 702 individuals from four Chinese cattle breeds. A novel single nucleotide
polymorphism (SNP) (IVS1 + 382T > C) was detected. The frequencies of genotype TC in four breeds were 0.000–0.222. Polymorphism
of the Hesx1 gene was shown to be associated with growth in the Nanyang breed. Individuals with genotype TC was significantly lower average
daily gain than TT at 18 months (P < 0.05). 相似文献
10.
Meixia Fang Qinghua Nie Chenlong Luo Dexiang Zhang Xiquan Zhang 《Molecular biology reports》2010,37(1):423-428
Ghrelin receptor (GHSR), or growth hormone secretagogue receptor, modulates many physiological effects by binding to its ligand
and therefore is a candidate gene for chicken production performance. In this study, five polymorphisms (four SNP and a ‘GGTACA’
indel) of GHSR gene were genotyped in a F2 full sib chicken population to investigate their associations with production traits. Results showed that c.739 + 726T > C
(M2) was significantly associated with body weight (BW) at 28 days (BW28), BW90, dressed weight, eviscerated weight, eviscerated
weight with giblet, breast muscle weight and leg muscle weight (P < 0.05). Meanwhile, T allele rather than C was positive for chicken body weight gain as individuals with CC had the lowest
value of all traits. Otherwise, no significant association of c.264G > A (M1), c.3211-196_3211-181delGGTACA (M3), c.3211 + 75C > T
(M4), and c.3211 + 150C > T (M5) with any growth and carcass traits was found. Haplotypes based on five polymorphisms were
significantly associated with hatch weight, BW7, BW14, BW21 and breast angle (P < 0.05), as well as BW28 (P < 0.01). Therefore, it was concluded that M2 of the GHSR gene and the analyzed haplotypes were associated with some chicken
growth and carcass traits. 相似文献
11.
Yujiao Qu Yanli Liu Liang Ma Sandra Sweeney Xianyong Lan Zhongqi Chen Zhuanjian Li Chuzhao Lei Hong Chen 《Molecular biology reports》2011,38(1):371-377
Butyrophilin (BTN1A1) and milk fat globule epidermal growth factor (EGF) 8 (MFG-E8) genes are both milk fat globule membrane proteins. BTN1A1 plays a key role in the secretion of milk lipid and production which has effects on performance traits, while the MFG-E8 is vital for the development of the mammary gland and phagocytic clearance of apoptotic cells. Therefore, BTN1A1 and MFG-E8 gene are candidate genes for quantitative traits in mammalian animals with respect to milk performance traits. The objective
of this study is to investigate variations in goat BTN1A1 and MFG-E8 gene and analyze their associations with growth trait and milk performance. In this study, the goat BTN1A1 gene showed a novel single-nucleotide polymorphism (SNP): XM_001494179:g.8659C>T, resulting in a missense mutation: CTT (Leu)>TTT
(Phe) at position 377 aa of the BTN1A1 (526 aa); the goat MFG-E8 gene showed four novel SNPs: NC_007319: g.843delA, 6417delC, 14892T>C and 14996A>C, only the 14892T>C result in a synonymous
mutation. The associations between genotypes and production traits were analyzed. Significant statistical results implied
that HinfI locus of BTN1A1 gene is associated with milk fat yield (P = 0.004), total solid (P = 0.002), solid-non fat (P = 0.018) and first milk yield (P = 0.030). The DA and EcoRV loci of MFG-E8 gene are associated with milk fat yield (DA locus: P = 0.000; EcoRV locus: P = 0.033) and total solid (DA locus: P = 0.002; EcoRV locus: P = 0.015) in the Xinong Saanen dairy goat. 相似文献
12.
Q. J. Jin J. J. Sun X. T. Fang C. L. Zhang L. Yang D. X. Chen X. Y. Shi Y. Du X. Y. Lan H. Chen 《Molecular biology reports》2011,38(5):3129-3135
Somatostatin (SST) and its receptors (SSTR1-5) appear to be important in central regulation of many metabolic systems that
affect growth, adiposity and nutrient absorption. In this study, we investigated polymorphisms within the caprine SST and SSTR1 genes and determined their relationship with growth traits. As there were no sequence information of the caprine SST and SSTR1 genes, we explored their DNA sequence and genomic organizations. The caprine SST gene is organized in two exons and is transcribed into an mRNA containing 351 bp of sequence coding for a protein of 116
amino acids. Its protein sequences showed substantial similarity (97–99%) to its respective orthologs from cattle, human and
mouse. We also cloned and sequenced a 1.2 kb DNA fragment which contained the major part of the coding region and 3′ UTR of
the caprine SSTR1 gene. We then detected the polymorphisms in these determined sequences by PCR-SSCP and DNA sequencing methods in 459 goats
from four breeds. Four SNPs (GU014693:g.647T>C, GU014693:g.844A>C, GU014693:g.970T>C, GU014693:g.1039T>A), segregating as
two haplotypes (T-A-T-T and C-C-C-A), were identified in intron 1 of the caprine SST gene and showed the associations to body length and body height (P < 0.05). Two SNPs (GU014695:g.801 C>T, GU014695:g.948 C>T) were identified in the caprine SSTR1 gene. Significant associations between the three genotypes of GU014695:801 C>T and body length, body height, and chest circumference
was observed (P < 0.05). These results suggest that the caprine SST and SSTR1 genes are strong candidate genes that influence growth traits in goat. 相似文献
13.
Polymorphisms of caprine <Emphasis Type="Italic">GDF</Emphasis>9 gene and their association with litter size in Jining Grey goats 总被引:1,自引:0,他引:1
Feng T Geng CX Lang XZ Chu MX Cao GL Di R Fang L Chen HQ Liu XL Li N 《Molecular biology reports》2011,38(8):5189-5197
The exons 1, 2 and flanking region of growth differentiation factor 9 (GDF9) gene in five randomly selected does of Jining Grey, Boer and Liaoning Cashmere goats were amplified and analyzed. Thirteen
nucleotide differences were identified in GDF9 gene between sheep (AF078545) and goats. Four SNPs (G3288A in intron 1, G423A, A959C [Gln320Pro] and G1189A [Val397Ile]
in exon 2) were detected in four goat breeds with different prolificacy, in which G3288A was a new SNP in goats. The results
showed that loci 3288, 423 and 1189 in Boer goats, loci 3288 and 423 in Guizhou White goats, loci 423 and 1189 in Liaoning
Cashmere goats were all in complete linkage disequilibrium (D′ = 1, r
2 = 1), respectively. In moderate (Boer goat) and low prolificacy (Liaoning Cashmere goat) breeds, linkage analysis indicated
that there were more fervent linkage disequilibrium among loci 3288, 423 and 1189 than high prolificacy (Jining Grey and Guizhou
White goats) breeds. For the 959 locus, the genotype distribution showed obvious difference between high prolificacy breeds
and moderate or low prolificacy breeds (P < 0.05 or P < 0.01). The Jining Grey goat does with genotype CC or AC had 0.81 (P < 0.01) or 0.63 (P < 0.01) kids more than those with genotype AA, respectively. The present study preliminarily showed an association between
allele C at 959 locus of GDF9 gene and high litter size in Jining Grey goats. These results provide further evidence that the GDF9 gene may be significantly correlated with high prolificacy in goats. 相似文献
14.
F. Y. Chen H. Niu J. Q. Wang C. Z. Lei X. Y. Lan C. L. Zhang M. J. Li L. S. Hua J. Wang H. Chen 《Molecular biology reports》2011,38(1):243-248
DLK1 and CLPG were located in DLK1-GTL2 imprinted cluster. They all affected muscle growth and meat tenderness. The functional importance of DLK1 and CLPG imply that the variation of the genes could affect the growth traits of animal. PCR-SSCP and sequencing were used to analyze
the four loci of DLK1 gene and CLPG gene in 1109 individuals, which belong to eight breeds/species of bovidae, including cattle, buffalo and yak. A synonymous
mutation (C451T) was detected in exon 5 of DLK1 in Qinchuan cattle, but didn’t change significantly with phenotypic traits. Three genotypes AA, AB and AC of CLPG were identified in Jiaxian cattle. The associations analyst of different genotypes showed that the individuals with genotypes
AA and AC had a greater body weight and longer body length than those with genotype AB (P < 0.05 and P < 0.01, respectively); the AA individuals were different from those AB (P < 0.05) in the circumference of cannon bone. No polymorphism was observed in the other populations at other loci. These results
were in agreement with the homology analysis: DLK1 and CLPG genes were in a highly conserved. 相似文献
15.
A 2 × 3 factorial arrangement of treatments was used to investigate the effects of different levels of copper (Cu, 0, 19,
and 38 mg/kg, dry matter (DM)) and molybdenum (Mo, 0 and 5 mg/kg, DM) supplements and an interaction of these two factors
on growth performance, nutrient digestibility, and cashmere and follicle characteristics in cashmere goats. Thirty-six Liaoning
cashmere goats (approximately 1.5 years of age; 27.53 ± 1.38 kg of body weight) were assigned randomly to one of six treatments
and fed with Chinese wildrye- and alfalfa hay-based treatment diets (the basal diet contained 4.72 mg Cu/kg, 1.65 mg Mo/kg,
and 0.21% S.). Body weight was measured on two consecutive days at the start and the end of the 70-day experimental period.
On day 30, the metabolism trial was conducted to study the effects of dietary Cu and Mo on nutrient digestibility. The cashmere
and skin samples were collected on day 70. Copper supplementation increased (P < 0.05) growth performance and fiber digestion, but there were no differences (P > 0.05) between Cu-supplemented groups. Addition of 19 mg Cu/kg DM increased (P < 0.05) cashmere growth length or growth rate by increasing the number of active secondary follicles. Molybdenum supplementation
decreased (P < 0.05) growth, but did not affect (P > 0.05) nutrient digestion, cashmere, and follicle characteristics. There is a tendency or significant interaction effect
of Cu and Mo on growth performance (P = 0.057), cashmere growth (P = 0.076), or diameter (P < 0.05) which might be accomplished by changing the number of secondary follicle and active secondary follicle, and secondary
to primary follicle ratio. In conclusion, the optimal supplemental Cu level for Liaoning cashmere goats fed with the basal
diet was 19 mg/kg DM (the total dietary Cu level of 23.72 mg/kg DM), while 38 mg Cu/kg DM supplementation was found to be
needed when 5 mg Mo/kg was added in the basal diet during the cashmere growing period. 相似文献
16.
Z. Y. Xu H. Yang Y. Z. Xiong C. Y. Deng F. E. Li M. G. Lei B. Zuo 《Molecular biology reports》2010,37(7):3609-3613
In this study, two novel SNPs (EU743939:g.5174T>C in intron 4 and EU743939:g.8350C>A in intron 7) in TNNI1 and one SNP (EU696779:g.1167C>T in intron 3) in TNNI2 were identified by PCR–RFLP (PCR restriction fragment length polymorphism) using XbaI, MspI and SmaI restriction enzyme, respectively. The allele frequencies of three novel SNPs were determined in the genetically diverse
pig breeds including ten Chinese indigenous pigs and three Western commercial pig breeds. Association analysis of the SNPs
with the carcass traits were conducted in a Large White × Meishan F2 pig population. The linkage of two SNPs (g.5174T>C and g.8350C>A) in TNNI1 gene had significant effect on fat percentage. Besides these, the g.5174T>C polymorphism was also significantly associated
with skin percentage (P < 0.05), shoulder fat thickness (P < 0.05) and backfat thickness between sixth and seventh ribs (P < 0.05). The significant effects of g.1167C>T polymorphism in TNNI2 gene on fat percentage (P < 0.01), lean meat percentage (P < 0.05), lion eye area (P < 0.05), thorax–waist backfat thickness (P < 0.01) and average backfat thickness (P < 0.05) were also found. 相似文献
17.
Polymorphisms of <Emphasis Type="Italic">STAT5A</Emphasis> gene and their association with milk production traits in Holstein cows 总被引:1,自引:0,他引:1
He X Chu MX Qiao L He JN Wang PQ Feng T Di R Cao GL Fang L An YF 《Molecular biology reports》2012,39(3):2901-2907
The STAT5A gene was studied as a candidate gene for five milk production traits (milk yield at 305 days, protein percentage, fat percentage,
lactose percentage and dry matter percentage) in Holstein cows. According to the sequence of bovine STAT5A gene, two pairs of primers (P1 and P2) were designed to detect polymorphisms of STAT5A gene in 401 Holstein cows by PCR-RFLP and PCR-SSCP. The results showed that the products amplified by primers P1 and P2 displayed
polymorphisms. For P1, three genotypes (AA, AG, and GG) were detected, and the frequency of AA/AG/GG was 0.252/0.486/0.262,
respectively. Sequence analysis revealed a single nucleotide substitution A–G at 14217 bp (GenBank NC_007317) of bovine STAT5A gene while compared GG genotype with AA genotype. The differences of the least squares means for the four milk production
traits (milk yield at 305 days, fat percentage, lactose percentage and dry matter percentage) between AA, AG and GG were not
significant (P > 0.05). Least squares mean of protein percentage for AG or GG was significantly higher than that for AA (P < 0.05); the difference of the least squares mean for protein percentage was not significant between AG and GG (P > 0.05). For P2, three genotypes (CC, CT, and TT) were detected in Holstein cows, and the frequency of CC/CT/TT was 0.751/0.234/0.015,
respectively. Sequencing revealed an insertion CCT at 17266 (NC_007317) of bovine STAT5A gene while compared CC genotype with TT genotype. The differences of the least squares means for the three milk production
traits (protein percentage, lactose percentage and dry matter percentage) between CC, CT and TT were not significant (P > 0.05). Least squares mean of milk yield at 305 days for TT or CT was significantly higher than that for CC (P < 0.05); the difference of the least squares mean for milk yield at 305 days was not significant between TT and CT (P > 0.05). Least squares mean of fat percentage for CC or CT was significantly higher than that for TT (P < 0.05); the difference of the least squares mean for fat percentage was not significant between CC and CT (P > 0.05). The results preliminarily indicated that allele G of A14217G polymorphic site of STAT5A gene is a potential DNA marker for improving protein percentage in dairy cattle, 17266indelCCT polymorphic site of STAT5A gene is a potential DNA marker for improving milk yield at 305 days and fat percentage in dairy cattle. 相似文献
18.
Yong Feng Liu Lin Sen Zan Kui Li Shuan Ping Zhao Ya Ping Xin Qing Lin Wan Qiang Tian Zhi Wei Wang 《Molecular biology reports》2010,37(1):429-434
Body measurement traits, influenced by genes and environmental factors, play numerous important roles in the value assessment
of productivity and economy. Growth differentiate factor 5 (GDF5), involved in the development and maintenance of bone and
cartilage, is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). In
this study, based on the PCR-RFLP technology, we discovered and evaluated the potential association of the single nucleotide
polymorphism (SNP) (T586C in exon 1) of the bovine GDF5 gene with body measurement traits in 985 Bos taurus breed, 42 Bos indicus breed and 76 Bos indicus × Bos taurus individuals. As the SNP marker, there were the significant effects on the Body length (BL) in the Bos taurus (BT) and Bos indicus × Bos taurus (BMY) populations (P < 0.05). In BT population, animals with the genotype TT had lower mean values for BL and Hip width (HW) than these with the
TC and CC genotype (P < 0.01). In BMY population, animals with the genotype TC had lower mean values for BL than these with the genotype CC (P < 0.05). These results suggest that the SNP of the GDF5 gene could be a very useful genetic marker for body measurement traits
in the bovine reproduction and breeding. 相似文献
19.
F. Li H. Chen C. Z. Lei G. Ren J. Wang Z. J. Li J. Q. Wang 《Molecular biology reports》2010,37(1):501-505
In this study, polymorphism in the exon 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 of bovine NUCB2 gene was detected by PCR-SSCP and DNA sequencing methods in 686 individuals from three Chinese cattle breeds. Two haplotypes
(M and N), three observed genotypes (MM, MN and NN) and two SNPs (NC_007313: g. 27451G>A, NC_007313: g. 27472T>C) were detected. The frequencies of haplotypes M and N in inland
Chinese three breeds were 0.531–0.721 and 0.279–0.469 respectively. The studied showed that Nanyang, Jiaxian Red and Qinchuan
cattle populations were in Hardy–Weinberg equilibrium at SNPs locus of NUCB2 gene (P > 0.05). Polymorphism of the NUCB2 gene was shown to be associated with growth traits in Qingchuan and Nanyang cattle breed. The linkage of two mutant sites
in the bovine NUCB2 gene had significant effects on body length, body weight, heart girth, and average daily gain at 24 months (P < 0.05). Results of this study suggested that the NUCB2-gene-specific SNP may be a useful marker for growth traits in future marker-assisted selection programmes in inland Chinese
cattle. 相似文献
20.
Analysis of caprine pituitary specific transcription factor-1 gene polymorphism in indigenous Chinese goats 总被引:1,自引:0,他引:1
Lan XY Li MJ Chen H Zhang LZ Jing YJ Wei TB Ren G Wang X Fang XT Zhang CL Lei CZ 《Molecular biology reports》2009,36(4):705-709
Since mutations on POU1F1 gene possibly resulted in deficiency of GH, PRL, TSH and POU1F1, this study revealed the polymorphism of goat POU1F1-AluI locus and analyzed the distribution of alleles on 13 indigenous Chinese goat breeds. The PCR-RFLP analysis showed the predominance
of TT genotype and the frequencies of allele T varied from 0.757 to 0.976 in the analyzed populations (SBWC, Bo, XH and HM).
Further study, distributions of genotypic and allelic frequencies at this locus were found to be significantly different among
populations based on a χ2-test (P < 0.001), suggesting that the breed factor significantly affected the molecular genetic character of POU1F1 gene. The genetic diversity analysis revealed that Chinese indigenous populations had a wide spectrum of genetic diversity
in goat POU1F1-AluI locus. However, the ANOVA analysis revealed no significant differences for gene homozygosty, gene heterozygosty, effective
allele numbers and PIC (polymorphism information content) among meat, dairy and cashmere utility types (P > 0.05), suggesting that goat utility types had no significant effect on the spectrum of genetic diversity.
X. Y. Lan and M. J. Li equally contributed to this work. 相似文献