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棉花离体培养纤维的研究进展 总被引:4,自引:0,他引:4
从试管棉纤维的诱导条件、影响试管棉纤维分化和伸长的因子以及其发育机制等方面进行了综述.试管棉纤维主要是通过胚珠及愈伤组织诱导形成,其影响因子主要是植物生长调节物质及外界环境.同时总结了目前棉花离体培养纤维中存在问题,并展望了棉花离体培养的前景和应用价值. 相似文献
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棉花组织培养研究的现状和前景 总被引:3,自引:0,他引:3
对棉花组织培养中胚珠(幼)培养和杂种植株获得,体细胞无性系变异和抗性突变体筛选,花药培养和单倍体育种,体细胞胚发生和人工种子的制作,离体棉纤维诱导和超级棉生产,原生质体培养和植株再生等研究进展,问题和前景作了概述。 相似文献
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梳唇石斛试管开花诱导和离体保存 总被引:1,自引:0,他引:1
对珍稀濒危药用植物梳唇石斛(Dendrobidium strongylanthum Rchb. f.)离体开花诱导和保存进行试验、观察和分析,研究基本培养基和植物生长调节剂对诱导离体开花的影响以及培养条件对离体保存的影响。结果显示,试管丛芽培养在MS+6-BA 2 mg/L+NAA 0.2 mg/L的培养基上,培养20 d试管开花的诱导率高达96%以上。试管丛芽接种在花宝1号3 g/L+NAA 1 mg/L+活性炭0.5 g/L培养基上,在12 ℃和HR 60%条件下保存,继代周期可延长至2年。该物种快速繁殖方法和保存技术的成功,为濒危植物梳唇石斛持续利用提供了技术支持。 相似文献
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以花生幼叶为外植体进行离体培养,研究BA浓度对花器官分化的影响并进一步观察试管内花器官的发育.结果表明:经MSB 1mg/LBA 0.5mg/LKIN 2mg/LIAA培养基诱导的愈伤组织,转接到附加1~3mg/LBA的MSB培养基上培养,均能直接诱导分化花器官,但2mg/LBA的诱导效率最高达21.13%;诱导分化的花器官转接到MSB培养基继续培养,部分花器官可以在试管内开花、受精、成针、结实.试验实现了以花生幼叶为外植体,在试管内完成诱导花芽、开花、受精、形成果针、子房膨大,直至形成荚果等过程,为离体条件下研究花生花器官分化、荚果及种子发育提供了技术体系和材料. 相似文献
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蝴蝶兰离体培养及其相关生物技术研究进展 总被引:1,自引:0,他引:1
崔广荣 《热带亚热带植物学报》2010,18(6):696-706
对蝴蝶兰(Phalaenopsis)的离体培养、褐化及控制、物理化学诱变、转基因研究进展进行了综述,包括蝴蝶兰离体培养中的外植体选择、体细胞胚胎及类原球茎诱导、培养基及培养条件、培养方式、褐化控制等关键因素,并概括了生物反应器、转基因、诱变育种等相关生物技术研究进展。成体植株的花梗、茎尖和种子以及试管苗的叶片、茎尖、根尖等均可作为外植体;影响外植体褐化的主要因素有培养基的种类、pH值、温度、外植体的生理状况等;蝴蝶兰诱变育种及转基因研究尚处于初步阶段。 相似文献
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影响棉纤维分化和发育的因素 总被引:17,自引:0,他引:17
影响棉纤维生长发育的主要因素是:基因型、激素、温度、水分、光照、授粉受精状况等。基因型决定棉纤维分化发育方式,内源或外源激素调控纤维分化、伸长、次生壁形成等发育过程。温度对纤维分化发育也有很大程度的影响。离体纤维生长发育除了受上述因素影响外,还受微量元素、维生素、NH4^+、CO2浓度等影响。 相似文献
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Native cotton fiber and in vitro cotton fiber that was induced from cotton ovule callus by suspension culture were observed using transmission electron microscope and scanning electron microscope. The ovule surface on the first day preanthesis was quite smooth. On the anthesis, it had a lot of protuberances. Two kinds of callus, smooth and rough were found. The microfibrils of callus was vertical to the cell long axis and they changed their orientations with the development of the in vitro cotton fiber: from the vertical to shallow spiral and then to parallel to the cell long axis. So was the native cotton fiber. It suggests that in vitro cotton fiber and native cotton fiber have similar development process. Compared with the ovule surface cell, most callus cells had smaller nuclear. During the development of the fiber, the plasm of native cotton fiber was denser than that of in vitro fiber, and it has more cellular organ than in vitro fiber. The cell wall of native cotton fiber was thicker and denser than that of the in vitro cotton fiber too. It suggests that the physiological activity of in vitro cotton fiber was less active than native cotton fiber. 相似文献
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棉纤维由棉胚珠表皮细胞分化生长而成,是研究细胞分化、细胞伸长等机理的良好材料。为了更好地研究它的分化和发育,人们建立了多种实验系统:离体胚珠培养系统、胚珠来源的单细胞悬浮培养系统、胚珠愈伤组织细胞来源的细胞悬浮培养系统,并对这些实验系统的特点对纤维细胞分化和生长,如培养基的配方、激素配比、pH值、抑制剂或促进剂的影响等进行了较为系统的研究。发现棉纤维的发育 相似文献
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Summary Abscisic acid (ABA) inhibits in vitro growth of cotton (Gossypium hirsutum L.) fiber and is effective only when applied during the first four days of culture started on the day of anthesis. Abscisic acid causes a small increase in potassium uptake by the ovules and also enhances leakage of potassium from them. During their period of rapid growth, fibers produced by ABA-treated ovules have a higher potassium content and a lower malate content as compared to fibers on untreated control ovules. Results are discussed in the light of earlier reports on the in vitro growth of cotton fiber and effects of abscisic acid on other plant tissues. It is suggested that ABA inhibits fiber growth, in part, by interfering with malate metabolism.Abbreviations ABA
abscisic acid
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- TFU
total fiber units 相似文献
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Adding gelling agents to cotton ovule culture media leads to subtle changes in fiber development 总被引:1,自引:0,他引:1
Barbara A. Triplett Damicca S. Johnson 《In vitro cellular & developmental biology. Plant》1999,35(3):265-270
Summary Young cotton (Gossypium hirsutum) ovules will produce fiber in vitro when floated on a defined culture medium. Our laboratory is interested in examining the
effects of altered gravity environments on fiber development as a model for the effects of gravity on cell expansion and cellulose
biosynthesis. Since liquid culture media are unsuitable for altered gravity experiments, addition of gelling agents to cotton
ovule culture media is necessary. In this study we have systematically examined the effects of four gelling agents at several
concentrations on fiber production in culture. A rapid screening method using toluidine blue O staining indicated that after
3 wk in culture, fiber growth on 0.15% (wt/vol) Phytagel™ medium was similar to fiber growth on liquid medium. More detailed
analysis of fiber development revealed that fiber length was not influenced by the addition of Phytagel™. Accumulation of
cellulose, however, was reduced 50–60% compared with fibers produced in liquid media after 3 wk in culture. The fiber cellulose
content rose with additional time in culture for both solid and liquid media treatments. By 4 wk in culture, the difference
in cellulose content of fiber cell walls grown on solid versus liquid media was less than 20%. This variance in growth response
on gelled media could be due to differences in media matric potential, to the immobility of ions trapped within the gel, or
to toxicity of contaminants copurifying with Phytagel™. By identifying why ovule growth and fiber cellulose biosynthesis are
reduced in cultures grown on gelled media, it will be possible to reveal new information about these processes in system that
is less complicated than physiological systems at the whole plant level.
Names of companies or commercial products are given solely for the purpose of providing specific information; their mention
does not imply recommendation or endorsement by the U.S. Department of Agriculture over others not mentioned. 相似文献
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Digital image analysis of expansion growth of cultured cotton ovules with fibers and their responses to ABA 总被引:1,自引:0,他引:1
Ling Fan Meng Lü Zhi-Yong Ni Wen-Ran Hu Juan Wang 《In vitro cellular & developmental biology. Plant》2011,47(3):369-374
Cotton ovule cultures have obvious advantages over whole plants when experimental protocols call for inhibitors, radio-labeled
precursors or controlled environmental conditions to be tested. The responses of ovule expansion growth and attached fiber
elongation to external factors require accurate measurement techniques. This paper presents a new method for digital image
analysis of the growth area of cotton ovules with fibers at high resolution. The method was characterized under constant conditions
and during dynamic responses to different levels of ABA (abscisic acid) treatment. The growth area was treated as area occupied
within the outline of the Petri dish image of the growing ovule with fibers. Growth area increase showed the same trends as
fiber length increase and was significantly correlated with the fiber length increase under different levels of ABA treatment
(r
2 = 0.97). This new analysis method provides a simple, noninvasive, and more accurate approach for growth analysis in the cotton
ovule culture system. Using this method, the effects of ABA on expansion growth of ovule with fibers were characterized. 相似文献
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棉纤维的分化起始是棉纤维形态建成的初始阶段,对棉纤维的产量和质量有重要影响。本文对棉纤维细胞起始分化的时空顺序、影响因素以及近年来棉纤维分化起始在分子水平上的研究进展进行了综述,以阐明细胞分化的内在机理,为通过遗传工程途径人为控制棉纤维生长发育、选育优良农艺性状的新种质提供依据。 相似文献