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| 桃叶杜鹃组织培养技术研究 | |
| 引用本文： | 苗永美,王永清,庄平,简兴.桃叶杜鹃组织培养技术研究[J].生物学杂志,2006,23(6):29-31. |
| 作者姓名： | 苗永美 王永清 庄平 简兴 |
| 作者单位： | 1. 四川农业大学林学园艺学院,雅安,625014;安徽科技学院生命科学学院,凤阳,233100 2. 四川农业大学林学园艺学院,雅安,625014 3. 中科院华西亚高山植物园,都江堰,611830 4. 安徽科技学院生命科学学院,凤阳,233100 |
| 摘 要： | 选用桃叶杜鹃试管苗的芽苗、叶片进行离体培养,通过正交试验设计并结合方差分析、多重比较从中选出了不同中间繁殖体的最佳培养基配方.试验结果表明:改良MS和6-BA及KT不适合桃叶杜鹃的组织培养,相对较低浓度的TDZ却能诱导植株分化,建立起桃叶杜鹃的离体培养体系,达到保护名贵杜鹃的种质资源,加速繁殖的目的. |
| 关 键 词： | 杜鹃 组织培养 TDZ 活性炭 |
| 文章编号： | 1008-9632(2006)06-0029-03 |
| 修稿时间： | 2006年3月27日 |
Study on technique tissue culture in Rhododendron anne Franch |
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| MIAO Yong-mei,WANG Yong-qing,ZHUANG Ping,JIAN Xing.Study on technique tissue culture in Rhododendron anne Franch[J].Journal of Biology,2006,23(6):29-31. | |
| Authors: | MIAO Yong-mei WANG Yong-qing ZHUANG Ping JIAN Xing |
| Abstract: | The shoots and leaves from tube seedlings of R..anne Franch were used as explants.The best medium for different explants in vitro culture was chosen by means of the orthogonal experiment design,analysis of variance and multiple comparisons.The test proved that amended MS,6-BA and KT did not suit for tissue culture of R.anne Franch.(Relatively) low concentration Thidiazuron induced plant differentiation.The established system could protect rare Rhododendron and its fast propagation. |
| Keywords: | Rhododendron tissue culture thidiazuron activated carbon |
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