共查询到20条相似文献,搜索用时 515 毫秒
1.
Kunert A Naz AA Dedeck O Pillen K Léon J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(5):683-695
The advanced backcross QTL (AB-QTL) strategy was utilised to locate quantitative trait loci (QTLs) for baking quality traits
in two BC2F3 populations of winter wheat. The backcrosses are derived from two German winter wheat cultivars, Batis and Zentos, and two
synthetic, hexaploid wheat accessions, Syn022 and Syn086. The synthetics originate from hybridisations of wild emmer (T. turgidum spp. dicoccoides) and T. tauschii, rather than from durum wheat and T. tauschii and thus allowed for the first time to test for exotic QTL effects on wheat genomes A and B in addition to genome D. The
investigated quality traits comprised hectolitre weight, grain hardness, flour yield Type 550, falling number, grain protein
content, sedimentation volume and baking volume. One hundred and forty-nine SSR markers were applied to genotype a total of
400 BC2F3 lines. For QTL detection, a mixed-model ANOVA was conducted, including the effects DNA marker, BC2F3 line, environment and marker × environment interaction. Overall 38 QTLs significant for a marker main effect were detected.
The exotic allele improved trait performance at 14 QTLs (36.8%), while the elite genotype contributed the favourable effect
at 24 QTLs (63.2%). The favourable exotic alleles were mainly associated with grain protein content, though the greatest improvement
of trait performance due to the exotic alleles was achieved for the traits falling number and sedimentation volume. At the
QTL on chromosome 4B the exotic allele increased the falling number by 19.6% and at the QTL on chromosome 6D the exotic allele
led to an increase of the sedimentation volume by 21.7%. The results indicate that synthetic wheat derived from wild emmer × T. tauschii carries favourable QTL alleles for baking quality traits, which might be useful for breeding improved wheat varieties by
marker-assisted selection. 相似文献
2.
Huang XQ Cöster H Ganal MW Röder MS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(8):1379-1389
Advanced backcross QTL (AB-QTL) analysis was used to identify quantitative trait loci (QTLs) for yield and yield components in a BC(2)F(2) population derived from a cross between the German winter wheat variety 'Prinz' and the synthetic wheat line W-7984 developed by CIMMYT. Two hundred and ten microsatellite markers were employed to genotype 72 pre-selected BC(2)F(2) plants and phenotypic data were collected for five agronomic traits from corresponding BC(2)F(3) families that were grown at four locations in Germany. Using single-marker regression and interval mapping, a total of 40 putative QTLs derived from W-7984 were detected, of which 11 were for yield, 16 for yield components, eight for ear emergence time and five for plant height. For 24 (60.0%) of them, alleles from the synthetic wheat W-7984 were associated with a positive effect on agronomic traits, despite the fact that synthetic wheat was overall inferior with respect to agronomic appearance and performance. The present study indicated that favorable QTL alleles could be transferred from wild relatives of wheat into an elite wheat variety for improvement of quantitative trait loci like yield by the advanced backcross QTL strategy and molecular breeding. To our knowledge, the results presented here were the first report on AB-QTL analysis in wheat. 相似文献
3.
Narasimhamoorthy B Gill BS Fritz AK Nelson JC Brown-Guedira GL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(5):787-796
Advanced backcross quantitative trait locus (AB-QTL) analysis was used to identify QTLs for yield and yield components in
a backcross population developed from a cross between hard red winter wheat (Triticum aestivum L.) variety Karl 92 and the synthetic wheat line TA 4152-4. Phenotypic data were collected for agronomic traits including
heading date, plant height, kernels per spike, kernel weight, tiller number, biomass, harvest index, test weight, grain yield,
protein content, and kernel hardness on 190 BC2F2:4 lines grown in three replications in two Kansas environments. Severity of wheat soilborne mosaic virus (WSBMV) reaction was
evaluated at one location. The population was genotyped using 151 microsatellite markers. Of the ten putative QTLs identified,
seven were located on homoeologous group 2 and group 3 chromosomes. The favorable allele was contributed by cultivated parent
Karl 92 at seven QTLs including a major one for WSBMV resistance, and by the synthetic parent at three QTLs: for grain hardness,
kernels per spike, and tiller number.
Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information
and does not imply recommendation or endorsement by the U.S. Department of Agriculture. 相似文献
4.
D. Bernacchi T. Beck-Bunn Y. Eshed J. Lopez V. Petiard J. Uhlig D. Zamir S. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(3):381-397
Advanced backcross QTL (AB-QTL) analysis is a new strategy for studying the effect of unadapted alleles on the agronomic
performance of elite cultivated lines. In this paper we report results from the application of the AB-QTL strategy to cultivated
tomato using the wild species Lycopersicon hirsutum LA1777 as the donor parent. RFLP genomic fingerprints were determined for 315 BC2 plants and phenotypic data were collected for 19 agronomic traits from approximately 200 derived BC3 lines which were grown in replicated field trials in three locations worldwide. Between 1 and 12 significant QTLs were identified
for each of the 19 traits evaluated, with a total of 121 QTLs identified for all traits. For 25 of the QTLs (20%) corresponding
to 12 traits (60%), the L. hirsutum allele was associated with an improvement of the trait from a horticultural perspective, despite the fact that L. hirsutum is overall phenotypically inferior to the elite parent. For example, L. hirsutum has fruit that remains green when ripe (lack of red pigment) yet alleles were found in this species that significantly increase
red color when transferred into cultivated tomatoes. Wild alleles were also associated with increases in total yield and soluble
solids (up to 15%) and brix×red yield (up to 41%). These results support the idea that one cannot predict the genetic potential
of exotic germplasm based on phenotype alone and that marker-based methods, such as the AB-QTL strategy, should be applied
to fully exploit exotic germplasm.
Received: 27 October 1997 / Accepted: 25 November 1997 相似文献
5.
Yuling Li Yongbin Dong Suzhenniu Niu Dongqun Cui Yanzhao Wang Yanyang Liu Mengguan Wei Xuehui Li 《Molecular breeding : new strategies in plant improvement》2008,21(1):1-14
Specific traits are an important consideration in plant breeding. In popcorn, inferior agronomic traits could be improved
using dent or flint corn backcrossed with popcorn. In this study, we used advanced backcross quantitative trait locus (AB-QTL)
analysis to identify trait-improving QTL alleles from a dent maize inbred Dan232, and compared the detection of QTL in the
BC2S1 population with QTL results using F2:3 families of the same population. Two hundred and twenty BC2S1 families developed from a cross between Dan232 and an elite popcorn inbred N04 were evaluated for nine plant traits in replicated
field trials under two environments. Using composite interval mapping (CIM), a total of 28 significant QTL were detected,
and of these, 23 (82.14%) had favorable alleles contributed by the dent corn parent Dan232. Nine QTL (32.14%) detected in
the BC2S1 population were also located in or near the same chromosome intervals in the F2:3 population. All of the favorable QTL alleles from Dan232 could be used in marker-assisted selection (MAS) to improve the
respective plant traits in popcorn breeding. In addition, their near isogenic lines (QTL-NILs) could be obtained through selfing
or another 1–2 backcross with N04. Also, N04 improved for the studied plant traits could be developed from the BC2S1 families used in this study. This study demonstrated that the AB-QTL method can be applied to identify favorable QTL from
dent corn inbred in popcorn breeding and, once identified, the alleles could be used in marker-assisted selection to improve
the respective plant traits. 相似文献
6.
Li YL Niu SZ Dong YB Cui DQ Wang YZ Liu YY Wei MG 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(1):129-140
Normal maize germplasm could be used to improve the grain yield of popcorn inbreds. Our first objective was to locate genetic
factors associated with trait variation and make first assessment on the efficiency of advanced backcross quantitative trait
locus (AB-QTL) analysis for the identification and transfer of favorable QTL alleles for grain yield components from the dent
corn inbred. A second objective was to compare the detection of QTL in the BC2F2 population with results using F2:3 lines of the same parents. Two hundred and twenty selected BC2F2 families developed from a cross between Dan232 and an elite popcorn inbred N04 were evaluated for six grain yield components
under two environments, and genotyped by means of 170 SSR markers. Using composite interval mapping (CIM), a total of 19 significant
QTL were detected. Eighteen QTL had favorable alleles contributed by the dent corn parent Dan232. Sixteen of these favorable
QTL alleles were not in the same or near marker intervals with QTL for popping characteristics. Six QTL were also detected
in the F2:3 population. Improved N04 could be developed from 210 and 208 families with higher grain weight per plant and/or 100-grain
weight, respectively, and 35 families with the same or higher popping expansion volume than N04. In addition, near isogenic
lines containing detected QTL (QTL-NILs) for grain weight per plant and/or 100-grain weight could be obtained from 12 families.
Our study demonstrated that the AB-QTL method can be applied to identify and manipulate favorable QTL alleles from normal
corn inbreds and combine QTL detection and popcorn breeding efficiently. 相似文献
7.
Quantitative trait loci for yield and related traits in the wheat population Ning7840 × Clark 总被引:1,自引:0,他引:1
Marza F Bai GH Carver BF Zhou WC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(4):688-698
Grain yield and associated agronomic traits are important factors in wheat (Triticum aestivum L.) improvement. Knowledge regarding the number, genomic location, and effect of quantitative trait loci (QTL) would facilitate
marker-assisted selection and the development of cultivars with desirable characteristics. Our objectives were to identify
QTLs directly and indirectly affecting grain yield expression. A population of 132 F12 recombinant inbred lines (RILs) was derived by single-seed descent from a cross between the Chinese facultative wheat Ning7840
and the US soft red winter wheat Clark. Phenotypic data were collected for 15 yield and other agronomic traits in the RILs
and parental lines from three locations in Oklahoma from 2001 to 2003. Twenty-nine linkage groups, consisting of 363 AFLP
and 47 SSR markers, were identified. Using composite interval mapping (CIM) analysis, 10, 16, 30, and 14 QTLs were detected
for yield, yield components, plant adaptation (shattering and lodging resistance, heading date, and plant height), and spike
morphology traits, respectively. The QTL effects ranged from 7 to 23%. Marker alleles from Clark were associated with a positive
effect for the majority of QTLs for yield and yield components, but gene dispersion was the rule rather than the exception
for this RIL population. Often, QTLs were detected in proximal positions for different traits. Consistent, co-localized QTLs
were identified in linkage groups 1AL, 1B, 4B, 5A, 6A, and 7A, and less consistent but unique QTLs were identified on 2BL,
2BS, 2DL, and 6B. Results of this study provide a benchmark for future efforts on QTL identification for yield traits. 相似文献
8.
Quantitative trait loci for yield and yield components in an Oryza sativa×Oryza rufipogon BC2F2 population evaluated in an upland environment 总被引:1,自引:0,他引:1
P. Moncada C. P. Martínez J. Borrero M. Chatel H. Gauch Jr E. Guimaraes J. Tohme S. R. McCouch 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(1):41-52
An advanced backcross breeding strategy was used to identify quantitative trait loci (QTLs) associated with eight agronomic
traits in a BC2F2 population derived from an interspecific cross between Caiapo, an upland Oryza sativa subsp. japonica rice variety from Brazil, and an accession of Oryza rufipogon from Malaysia. Caiapo is one of the most-widely grown dryland cultivars in Latin America and may be planted as a monoculture
or in a multicropping system with pastures. The objectives of this study were: (1) to determine whether trait-enhancing QTLs
from O. rufipogon would be detected in 274 BC2F2 families grown under the drought-prone, acid soil conditions to which Caiapo was adapted, (2) to compare the performance
with and without pasture competition, and (3) to compare putative QTL-containing regions identified in this study with those
previously reported for populations adapted to irrigated, low-land conditions. Based on analyses of 125 SSLP and RFLP markers
distributed throughout the genome and using single-point, interval, and composite interval mapping, two putative O. rufipogon derived QTLs were detected for yield, 13 for yield components, four for maturity and six for plant height.We conclude that
advanced backcross QTL analysis offers a useful germplasm enhancement strategy for the genetic improvement of cultivars adapted
to stress-prone environments. Although the phenotypic performance of the wild germplasm would not suggest its value as a breeding
parent, it is noteworthy that 56% of the trait-enhancing QTLs identified in this study were derived from O. rufipogon. This figure is similar to the 51% of favorable QTLs derived from the same parent in crosses with a high-yielding hybrid
rice cultivar evaluated under irrigated conditions in a previous study. In conclusion, parallel studies in rice using AB-QTL
analysis provide increasing evidence that certain regions of the rice genome are likely to harbor genes of interest for plant
improvement in multiple environments.
Received: 3 September 1999 / Accepted: 16 May 2000 相似文献
9.
Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.) 总被引:13,自引:0,他引:13
Huang XQ Cloutier S Lycar L Radovanovic N Humphreys DG Noll JS Somers DJ Brown PD 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(4):753-766
Development of high-yielding wheat varieties with good end-use quality has always been a major concern for wheat breeders. To genetically dissect quantitative trait loci (QTLs) for yield-related traits such as grain yield, plant height, maturity, lodging, test weight and thousand-grain weight, and for quality traits such as grain and flour protein content, gluten strength as evaluated by mixograph and SDS sedimentation volume, an F1-derived doubled haploid (DH) population of 185 individuals was developed from a cross between a Canadian wheat variety “AC Karma” and a breeding line 87E03-S2B1. A genetic map was constructed based on 167 marker loci, consisting of 160 microsatellite loci, three HMW glutenin subunit loci: Glu-A1, Glu-B1 and Glu-D1, and four STS-PCR markers. Data for investigated traits were collected from three to four environments in Manitoba, Canada. QTL analyses were performed using composite interval mapping. A total of 50 QTLs were detected, 24 for agronomic traits and 26 for quality-related traits. Many QTLs for correlated traits were mapped in the same genomic regions forming QTL clusters. The largest QTL clusters, consisting of up to nine QTLs, were found on chromosomes 1D and 4D. HMW glutenin subunits at Glu-1 loci had the largest effect on breadmaking quality; however, other genomic regions also contributed genetically to breadmaking quality. QTLs detected in the present study are compared with other QTL analyses in wheat. 相似文献
10.
Ratnam Wickneswari M. A. R. Bhuiyan Sabu Kalluvettankuzhy K. Li Sze Lim Michael J. Thomson Md. Kairudin Narimah Md. Zain Abdullah 《Plant Molecular Biology Reporter》2012,30(4):929-939
A backcross breeding strategy was used to identify quantitative trait loci (QTLs) associated with 14 traits in a BC2F2 population derived from a cross between MR219, an indica rice cultivar and an accession of Oryza rufipogon (IRGC 105491). A total of 261 lines were genotyped with 96 microsatellite markers and evaluated for plant morphology, yield components and growth period. The genetic linkage map generated for this population with an average interval size of 16.2?cM, spanning 1,553.4?cM (Kosambi) of the rice genome. Thirty-eight QTLs were identified with composite interval mapping (CIM), whereas simple interval mapping (SIM) resulted in 47 QTLs (LOD >3.0). The O. rufipogon allele was favourable for 59% of QTLs detected through CIM. Of 261 BC2F2 families, 26 advanced backcross breeding lines (BC2F5) were used for QTL validation. These lines were selected on the basis of the yield traits potentiality in BC2F3 and BC2F4 generations. The field trial was conducted at three different locations in Malaysia using randomized complete block design with three replications. Trait based marker analysis was done for QTL determination. Twenty-five QTLs were detected in BC2F5 generation whereas 29 QTLs were detected in BC2F2 generation of the same population. Two QTLs (qPL-1 and qSPL-7) were not considered for validation due to their low R 2 values and two QTLs (qPSS-3-2 and qGW-3-2) were not detected in the BC2F5 population. Fifteen QTLs showed the beneficial effect to enhance the trait value of the breeding lines. QTL validation aided to select the promising lines for further utilization. 相似文献
11.
D. Bernacchi T. Beck-Bunn D. Emmatty Y. Eshed S. Inai J. Lopez V. Petiard H. Sayama J. Uhlig D. Zamir S. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1191-1196
Improved-processing tomato lines were produced by the molecular breeding strategy of advanced backcross QTL (AB-QTL) analysis.
These near-isogenic lines (NILs) contained unique introgressions of wild alleles originating from two donor wild species,
Lycopersicon hirsutum (LA1777) and L. pimpinellifolium (LA1589). Wild alleles targeted for trait improvement were selected on the basis of previously published replicated QTL data
obtained from advanced backcross populations for a battery of important agronomic traits. Twenty three NILs were developed
for 15 genomic regions which were predicted to contain 25 quantitative trait factors for the improvement of seven agronomic
traits: total yield, red yield, soluble solids, brix×red yield, viscosity, fruit color, and fruit firmness. An evaluation
of the agronomic performance of the NILs in five locations worldwide revealed that 22 out of the 25 (88%) quantitative factors
showed the phenotypic improvement predicted by QTL analysis of the BC3 populations, as NILs in at least one location. Per-location gains over the elite control ranged from 9% to 59% for brix×red
yield; 14% to 33% for fruit color; 17% to 34% for fruit firmness; 6% to 22% for soluble-solids content; 7% to 22% for viscosity;
15% to 48% for red yield, and 20% to 28% for total yield. The inheritance of QTLs, the implementation of the AB-QTL methodology
for characterizing unadapted germplasm and the applicability of this method to other crops are discussed.
Theor Appl Genet (1998) 97 : 170–180
Received: 27 October 1997 / Accepted: 25 November 1997 相似文献
12.
V TALAMÉ M C SANGUINETI E CHIAPPARINO H BAHRI M BEN SALEM B P FORSTER R P ELLIS S RHOUMA W ZOUMAROU R WAUGH R TUBEROSA 《The Annals of applied biology》2004,144(3):309-319
Advanced backcross QTL (AB-QTL) analysis was deployed to identify allelic variation in wild barley (Hordeum vulgare ssp. spontaneum) of value in the improvement of grain yield and other agronomically important traits in barley (Hordeum vulgare ssp. vulgare) grown under conditions of water deficit in Mediterranean countries. A population of 123 double haploid (DH) lines obtained from BC1F2 plants derived from a cross between Barke (European two-row cultivar) and HOR11508 (wild barley accession) were tested in replicated field trials, under varying conditions of water availability in Italy, Morocco and Tunisia, for seven quantitative traits. Significant QTL effects at one (P 0.001) or more trial sites (P 0.01) were identified for all traits. At 42 (52%) of the 80 putative QTLs identified, the allele increasing a “traits' value” was contributed by H. spontaneum. For example, though the majority (67%) of QTL alleles increasing grain yield were contributed by H. vulgare, H. spontaneum contributed the alleles increasing grain yield at six regions on chromosomes 2H, 3H, 5H and 7H. Among them, two QTLs (associated to Bmac0093 on chromosome 2H and to Bmac0684 on chromosome 5H) were identified in all three locations and had the highest additive effects. The present study shows the validity of deploying AB-QTL analysis for identifying favourable QTL alleles from wild germplasm and indicates its potential as an enhancement strategy for the genetic improvement of cultivars better adapted to drought-prone environments. 相似文献
13.
D. Bernacchi T. Beck-Bunn D. Emmatty Y. Eshed S. Inai J. Lopez V. Petiard H. Sayama J. Uhlig D. Zamir S. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):170-180
Improved-processing tomato lines were produced by the molecular breeding strategy of advanced backcross QTL (AB-QTL) analysis.
These near-isogenic lines (NILs) contained unique introgressions of wild alleles originating from two donor wild species,
Lycopersicon hirsutum (LA1777) and L. pimpinellifolium (LA1589). Wild alleles targeted for trait improvement were selected on the basis of previously published replicated QTL data
obtained from advanced backcross populations for a battery of important agronomic traits. Twenty three NILs were developed
for 15 genomic regions which were predicted to contain 25 quantitative trait factors for the improvement of seven agronomic
traits: total yield, red yield, soluble solids, brix×red yield, viscosity, fruit color, and fruit firmness. An evaluation
of the agronomic performance of the NILs in five locations worldwide revealed that 22 out of the 25 (88%) quantitative factors
showed the phenotypic improvement predicted by QTL analysis of the BC3 populations, as NILs in at least one location. Per-location gains over the elite control ranged from 9% to 59% for brix×red
yield; 14% to 33% for fruit color; 17% to 34% for fruit firmness; 6% to 22% for soluble-solids content; 7% to 22% for viscosity;
15% to 48% for red yield, and 20% to 28% for total yield. The inheritance of QTLs, the implementation of the AB-QTL methodology
for characterizing unadapted germplasm and the applicability of this method to other crops are discussed.
Received: 27 October 1997 / Accepted: 25 November 1997 相似文献
14.
Septiningsih EM Prasetiyono J Lubis E Tai TH Tjubaryat T Moeljopawiro S McCouch SR 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(8):1419-1432
A BC2F2 population developed from an interspecific cross between Oryza sativa (cv IR64) and O. rufipogon (IRGC 105491) was used in an advanced backcross QTL analysis to identify and introduce agronomically useful genes from this wild relative into the cultivated gene pool. The objectives of this study were: (1) to identify putative yield and yield component QTLs that can be useful to improve the elite cultivar IR64; (2) to compare the QTLs within this study with previously reported QTLs in rice as the basis for identifying QTLs that are stable across different environments and genetic backgrounds; and (3) to compare the identified QTLs with previously reported QTLs from maize to examine the degree of QTL conservation across the grass family. Two hundred eighty-five families were evaluated in two field environments in Indonesia, with two replications each, for 12 agronomic traits. A total of 165 markers consisting of 131 SSRs and 34 RFLPs were used to construct the genetic linkage map. By employing interval mapping and composite interval mapping, 42 QTLs were identified. Despite its inferior performance, 33% of the QTL alleles originating from O. rufipogon had a beneficial effect for yield and yield components in the IR64 background. Twenty-two QTLs (53.4%) were located in similar regions as previously reported rice QTLs, suggesting the existence of stable QTLs across genetic backgrounds and environments. Twenty QTLs (47.6%) were exclusively detected in this study, uncovering potentially novel alleles from the wild, some of which might improve the performance of the tropical indica variety IR64. Additionally, several QTLs for plant height, grain weight, and flowering time detected in this study corresponded to homeologous regions in maize containing previously detected maize QTLs for these traits. 相似文献
15.
von Korff M Wang H Léon J Pillen K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(7):1221-1231
The objective of the present study was to identify favourable exotic Quantitative Trait Locus (QTL) alleles for the improvement
of agronomic traits in the BC2DH population S42 derived from a cross between the spring barley cultivar Scarlett and the wild barley accession ISR42-8 (Hordeum vulgare ssp. spontaneum). QTLs were detected as a marker main effect and/or a marker × environment interaction effect (M × E) in a three-factorial ANOVA. Using field data of up to eight environments and genotype data of 98 SSR loci, we detected 86
QTLs for nine agronomic traits. At 60 QTLs the marker main effect, at five QTLs the M × E interaction effect, and at 21 QTLs both the effects were significant. The majority of the M × E interaction effects were due to changes in magnitude and are, therefore, still valuable for marker assisted selection across
environments. The exotic alleles improved performance in 31 (36.0%) of 86 QTLs detected for agronomic traits. The exotic alleles
had favourable effects on all analysed quantitative traits. These favourable exotic alleles were detected, in particular on
the short arm of chromosome 2H and the long arm of chromosome 4H. The exotic allele on 4HL, for example, improved yield by
7.1%. Furthermore, the presence of the exotic allele on 2HS increased the yield component traits ears per m2 and thousand grain weight by 16.4% and 3.2%, respectively. The present study, hence, demonstrated that wild barley does harbour
valuable alleles, which can enrich the genetic basis of cultivated barley and improve quantitative agronomic traits. 相似文献
16.
Frary A Fulton TM Zamir D Tanksley SD 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(3):485-496
In this study, the advanced backcross QTL (AB-QTL) mapping strategy was used to identify loci for yield, processing and fruit quality traits in a population derived from the interspecific cross Lycopersicon esculentum E6203 × Lycopersicon pennellii accession LA1657. A total of 175 BC2 plants were genotyped with 150 molecular markers and BC2F1 plots were grown and phenotyped for 25 traits in three locations in Israel and California, U.S.A. A total of 84 different QTLs were identified, 45% of which have been possibly identified in other wild-species-derived populations of tomato. Moreover, three fruit-weight/size and shape QTLs (fsz2b.1, fw3.1/fsz3.1 and fs8.1) appear to have putative orthologs in the related solanaceous species, pepper and eggplant. For the 23 traits for which allelic effects could be deemed as favorable or unfavorable, 26% of the identified loci had L. pennellii alleles that enhanced the performance of the elite parent. Alleles that could be targeted for further introgression into cultivated tomato were also identified.Communicated by G. Wenzel 相似文献
17.
Zheng X Wu JG Lou XY Xu HM Shi CH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(3):335-342
Investigations to identify quantitative trait loci (QTLs) governing cooking quality traits including amylose content, gel
consistency and gelatinization temperature (expressed by the alkali spread value) were conducted using a set of 241 RIL populations
derived from an elite hybrid cross of “Zhenshan 97” × “Minghui 63” and their reciprocal backcrosses BC1F1 and BC2F1 populations in two environments. QTLs and QTL × environment interactions were analyzed by using the genetic model with endosperm
and maternal effects and environmental interaction effects on quantitative traits of seed in cereal crops. The results suggested
that a total of seven QTLs were associated with cooking quality of rice, which were subsequently mapped to chromosomes 1,
4 and 6. Six of these QTLs were also found to have environmental interaction effects. 相似文献
18.
K. Kato H. Miura S. Sawada 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(7):1114-1121
Chromosome 5A of wheat is known to carry a number of genes affecting adaptability and productivity. To localize quantitative
trait loci (QTLs) controlling grain yield and its components, an RFLP map was constructed from 118 single-chromosome recombinant
lines derived from the F1 between Chinese Spring (Cappelle-Desprez 5A) and Chinese Spring (Triticum spelta 5A). The map was combined with the field-trial data scored over 3 years. A total of five regions in chromosome 5A contributed
effects on yield traits. Increases in grain yield, 50-grain weight and spikelet number/ear were determined by complementary
QTL alleles from both parents. The effects associated with the vernalization requirement gene Vrn-A1 or a closely linked QTL were significant only in the favorable growing season where the later-flowering vrn-A1 allele from Cappelle-Desprez 5A produced a higher tiller number/plant and spikelet number/ear. The effects of the ear morphology
gene q or closely linked QTL(s) were detected for grain yield and ear grain weight. Three other QTLs with minor effects were dispersed
along chromosome 5A. These QTLs had large interactions with years due to changes in the magnitude of the significant response.
The alleles from T. spelta, however, conferred a higher yield performance.
Received: 18 August 1999 / Accepted: 25 March 2000 相似文献
19.
Characterization and precise mapping of a QTL increasing spike number with pleiotropic effects in wheat 总被引:2,自引:0,他引:2
Shimin Deng Xinru Wu Yuye Wu Ronghua Zhou Honggang Wang Jizeng Jia Shubing Liu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(2):281-289
Tiller number (TN) and spike number per plant (SN) are key components of grain yield and/or biomass in wheat. In this study,
an introgression line 05210, developed by introgression of chromosomal segments from a synthetic exotic wheat Am3 into an
elite cultivar Laizhou953, showed a significantly increased TN and SN, but shorter spike length (SL) and fewer grain number
per spike (GNS) than Laizhou953. To investigate the quantitative trait locus (QTL) responsible for these variations, the introgressed
segments in 05210 were screened by SSR markers and one follow-up segregation population was developed from the cross 05210/Laizhou953.
The population showed 3:1 segregation ratios for SN, SL and GNS, indicating that QTLs for these traits have been dissected
into single Mendelian factors. Bulked segregation analysis showed that the markers located on the 4B introgressed segment
were polymorphic between the two bulks. Therefore, they were further analyzed in the F2 population to construct a linkage map. Three new QTLs, QSn.sdau-4B, QSl.sdau-4B and QGns.sdau-4B, were detected for SN, SL and GNS, respectively, which explained a large portion of the phenotypic variation (30.1–67.6%)
for these traits with overlapping peaks. Correlation analysis and multiple-trait, multiple-interval mapping (MMIM) suggested
pleiotropic effects of the QTL on SN, SL and GNS. Therefore, the QTL was designated as QSn.sdau-4B. By a progeny test based on F3 families using SN, the QTL was mapped as a Mendelian factor to the proximal region of 4BL. It is a key QTL responsible for
variation in spike number and size, which had not been reported previously. Thus, it is an important QTL for wheat to achieve
high and stable biomass and grain yield. Dissection and mapping of this QTL as a Mendelian factor laid a solid foundation
for map-based cloning of grain yield-related QTLs in wheat. 相似文献
20.
Dynamic QTL analysis of the Na+ content,K+ content,and Na+/K+ ratio in rice roots during the field growth under salt stress 总被引:1,自引:0,他引:1
J. Sun D. T. Zou F. S. Luan H. W. Zhao J. G. Wang H. L. Liu D. W. Xie D. Q. Su J. Ma Z. L. Liu 《Biologia Plantarum》2014,58(4):689-696
Rice (Oryza sativa L.) is seriously impacted by global soil salinization. To determine the quantitative trait loci (QTLs) related to salt tolerance in rice roots, F2:3 and BC1F2:3 populations derived from a cross between the cv. Dongnong 425 of high quality and yield and the salt-tolerant cv. Changbai 10, were studied at different development stages. Two genetic linkage maps of F2:3 and BC1F2:3 populations were constructed. A 66 mM NaCl solution was used to irrigate the field and to analyze the dynamic QTL of some rice root traits. Using unconditional and conditional QTL mapping methods, 30 unconditional QTLs and 16 conditional QTLs related to the 6 root traits were detected on the 9 rice chromosomes during different developmental stages. Fourteen pairs of unconditional and conditional QTLs were detected at the identical developmental stage in the identical population. A number of QTLs were detected at different developmental stages, however, many did not appear at the last stage. Remarkably, qRKC1 appeared continuously at multiple stages in both the populations suggesting its key role in regulating the salt tolerance of rice roots. 相似文献