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1.
D. Bernacchi T. Beck-Bunn D. Emmatty Y. Eshed S. Inai J. Lopez V. Petiard H. Sayama J. Uhlig D. Zamir S. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1191-1196
Improved-processing tomato lines were produced by the molecular breeding strategy of advanced backcross QTL (AB-QTL) analysis.
These near-isogenic lines (NILs) contained unique introgressions of wild alleles originating from two donor wild species,
Lycopersicon hirsutum (LA1777) and L. pimpinellifolium (LA1589). Wild alleles targeted for trait improvement were selected on the basis of previously published replicated QTL data
obtained from advanced backcross populations for a battery of important agronomic traits. Twenty three NILs were developed
for 15 genomic regions which were predicted to contain 25 quantitative trait factors for the improvement of seven agronomic
traits: total yield, red yield, soluble solids, brix×red yield, viscosity, fruit color, and fruit firmness. An evaluation
of the agronomic performance of the NILs in five locations worldwide revealed that 22 out of the 25 (88%) quantitative factors
showed the phenotypic improvement predicted by QTL analysis of the BC3 populations, as NILs in at least one location. Per-location gains over the elite control ranged from 9% to 59% for brix×red
yield; 14% to 33% for fruit color; 17% to 34% for fruit firmness; 6% to 22% for soluble-solids content; 7% to 22% for viscosity;
15% to 48% for red yield, and 20% to 28% for total yield. The inheritance of QTLs, the implementation of the AB-QTL methodology
for characterizing unadapted germplasm and the applicability of this method to other crops are discussed.
Theor Appl Genet (1998) 97 : 170–180
Received: 27 October 1997 / Accepted: 25 November 1997 相似文献
2.
D. Bernacchi T. Beck-Bunn D. Emmatty Y. Eshed S. Inai J. Lopez V. Petiard H. Sayama J. Uhlig D. Zamir S. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):170-180
Improved-processing tomato lines were produced by the molecular breeding strategy of advanced backcross QTL (AB-QTL) analysis.
These near-isogenic lines (NILs) contained unique introgressions of wild alleles originating from two donor wild species,
Lycopersicon hirsutum (LA1777) and L. pimpinellifolium (LA1589). Wild alleles targeted for trait improvement were selected on the basis of previously published replicated QTL data
obtained from advanced backcross populations for a battery of important agronomic traits. Twenty three NILs were developed
for 15 genomic regions which were predicted to contain 25 quantitative trait factors for the improvement of seven agronomic
traits: total yield, red yield, soluble solids, brix×red yield, viscosity, fruit color, and fruit firmness. An evaluation
of the agronomic performance of the NILs in five locations worldwide revealed that 22 out of the 25 (88%) quantitative factors
showed the phenotypic improvement predicted by QTL analysis of the BC3 populations, as NILs in at least one location. Per-location gains over the elite control ranged from 9% to 59% for brix×red
yield; 14% to 33% for fruit color; 17% to 34% for fruit firmness; 6% to 22% for soluble-solids content; 7% to 22% for viscosity;
15% to 48% for red yield, and 20% to 28% for total yield. The inheritance of QTLs, the implementation of the AB-QTL methodology
for characterizing unadapted germplasm and the applicability of this method to other crops are discussed.
Received: 27 October 1997 / Accepted: 25 November 1997 相似文献
3.
Maria von Korff Huajun Wang Jens Léon Klaus Pillen 《Molecular breeding : new strategies in plant improvement》2008,21(1):81-93
Malting quality is genetically determined by the complex interaction of numerous traits which are expressed prior to and,
in particular, during the malting process. Here, we applied the advanced backcross quantitative trait locus (AB-QTL) strategy
(Tanksley and Nelson, Theor Appl Genet 92:191–203, 1996), to detect QTLs for malting quality traits and, in addition, to identify favourable exotic alleles for the improvement of
malting quality. For this, the BC2DH population S42 was generated from a cross between the spring barley cultivar Scarlett and the wild barley accession ISR42-8
(Hordeum vulgare ssp. spontaneum). A QTL analysis in S42 for seven malting parameters measured in two different environments yielded 48 QTLs. The exotic genotype
improved the trait performance at 18 (37.5%) of 48 QTLs. These favourable exotic alleles were detected, in particular, on
the chromosome arms 3HL, 4HS, 4HL and 6HL. The exotic allele on 4HL, for example, improved α-amylase activity by 16.3%, fermentability
by 0.8% and reduced raw protein by 2.4%. On chromosome 6HL, the exotic allele increased α-amylase by 16.0%, fermentability
by 1.3%, friability by 7.3% and reduced viscosity by 2.9%. Favourable transgressive segregation, i.e. S42 lines exhibiting
significantly better performance than the recurrent parent Scarlett, was recorded for four traits. For α-amylase, fermentability,
fine-grind extract and VZ45 20, 16, 2 and 26 S42 lines, respectively, surpassed the recurrent parent Scarlett. The present
study hence demonstrates that wild barley does harbour valuable alleles, which can enrich the genetic basis of cultivated
barley and improve malting quality traits. 相似文献
4.
Kunert A Naz AA Dedeck O Pillen K Léon J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(5):683-695
The advanced backcross QTL (AB-QTL) strategy was utilised to locate quantitative trait loci (QTLs) for baking quality traits
in two BC2F3 populations of winter wheat. The backcrosses are derived from two German winter wheat cultivars, Batis and Zentos, and two
synthetic, hexaploid wheat accessions, Syn022 and Syn086. The synthetics originate from hybridisations of wild emmer (T. turgidum spp. dicoccoides) and T. tauschii, rather than from durum wheat and T. tauschii and thus allowed for the first time to test for exotic QTL effects on wheat genomes A and B in addition to genome D. The
investigated quality traits comprised hectolitre weight, grain hardness, flour yield Type 550, falling number, grain protein
content, sedimentation volume and baking volume. One hundred and forty-nine SSR markers were applied to genotype a total of
400 BC2F3 lines. For QTL detection, a mixed-model ANOVA was conducted, including the effects DNA marker, BC2F3 line, environment and marker × environment interaction. Overall 38 QTLs significant for a marker main effect were detected.
The exotic allele improved trait performance at 14 QTLs (36.8%), while the elite genotype contributed the favourable effect
at 24 QTLs (63.2%). The favourable exotic alleles were mainly associated with grain protein content, though the greatest improvement
of trait performance due to the exotic alleles was achieved for the traits falling number and sedimentation volume. At the
QTL on chromosome 4B the exotic allele increased the falling number by 19.6% and at the QTL on chromosome 6D the exotic allele
led to an increase of the sedimentation volume by 21.7%. The results indicate that synthetic wheat derived from wild emmer × T. tauschii carries favourable QTL alleles for baking quality traits, which might be useful for breeding improved wheat varieties by
marker-assisted selection. 相似文献
5.
Frary A Fulton TM Zamir D Tanksley SD 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(3):485-496
In this study, the advanced backcross QTL (AB-QTL) mapping strategy was used to identify loci for yield, processing and fruit quality traits in a population derived from the interspecific cross Lycopersicon esculentum E6203 × Lycopersicon pennellii accession LA1657. A total of 175 BC2 plants were genotyped with 150 molecular markers and BC2F1 plots were grown and phenotyped for 25 traits in three locations in Israel and California, U.S.A. A total of 84 different QTLs were identified, 45% of which have been possibly identified in other wild-species-derived populations of tomato. Moreover, three fruit-weight/size and shape QTLs (fsz2b.1, fw3.1/fsz3.1 and fs8.1) appear to have putative orthologs in the related solanaceous species, pepper and eggplant. For the 23 traits for which allelic effects could be deemed as favorable or unfavorable, 26% of the identified loci had L. pennellii alleles that enhanced the performance of the elite parent. Alleles that could be targeted for further introgression into cultivated tomato were also identified.Communicated by G. Wenzel 相似文献
6.
von Korff M Wang H Léon J Pillen K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(7):1221-1231
The objective of the present study was to identify favourable exotic Quantitative Trait Locus (QTL) alleles for the improvement
of agronomic traits in the BC2DH population S42 derived from a cross between the spring barley cultivar Scarlett and the wild barley accession ISR42-8 (Hordeum vulgare ssp. spontaneum). QTLs were detected as a marker main effect and/or a marker × environment interaction effect (M × E) in a three-factorial ANOVA. Using field data of up to eight environments and genotype data of 98 SSR loci, we detected 86
QTLs for nine agronomic traits. At 60 QTLs the marker main effect, at five QTLs the M × E interaction effect, and at 21 QTLs both the effects were significant. The majority of the M × E interaction effects were due to changes in magnitude and are, therefore, still valuable for marker assisted selection across
environments. The exotic alleles improved performance in 31 (36.0%) of 86 QTLs detected for agronomic traits. The exotic alleles
had favourable effects on all analysed quantitative traits. These favourable exotic alleles were detected, in particular on
the short arm of chromosome 2H and the long arm of chromosome 4H. The exotic allele on 4HL, for example, improved yield by
7.1%. Furthermore, the presence of the exotic allele on 2HS increased the yield component traits ears per m2 and thousand grain weight by 16.4% and 3.2%, respectively. The present study, hence, demonstrated that wild barley does harbour
valuable alleles, which can enrich the genetic basis of cultivated barley and improve quantitative agronomic traits. 相似文献
7.
Narasimhamoorthy B Gill BS Fritz AK Nelson JC Brown-Guedira GL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(5):787-796
Advanced backcross quantitative trait locus (AB-QTL) analysis was used to identify QTLs for yield and yield components in
a backcross population developed from a cross between hard red winter wheat (Triticum aestivum L.) variety Karl 92 and the synthetic wheat line TA 4152-4. Phenotypic data were collected for agronomic traits including
heading date, plant height, kernels per spike, kernel weight, tiller number, biomass, harvest index, test weight, grain yield,
protein content, and kernel hardness on 190 BC2F2:4 lines grown in three replications in two Kansas environments. Severity of wheat soilborne mosaic virus (WSBMV) reaction was
evaluated at one location. The population was genotyped using 151 microsatellite markers. Of the ten putative QTLs identified,
seven were located on homoeologous group 2 and group 3 chromosomes. The favorable allele was contributed by cultivated parent
Karl 92 at seven QTLs including a major one for WSBMV resistance, and by the synthetic parent at three QTLs: for grain hardness,
kernels per spike, and tiller number.
Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information
and does not imply recommendation or endorsement by the U.S. Department of Agriculture. 相似文献
8.
Huang XQ Kempf H Ganal MW Röder MS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(5):933-943
We report here the second advanced backcross quantitative trait locus (AB-QTL) analysis carried out in winter wheat. Seven agronomic traits were studied in a BC2F1population derived from a cross between the German winter wheat variety Flair and the synthetic wheat line XX86 developed in Japan. We selected 111 BC2F1 lines and genotyped these with 197 microsatellite markers. Field data for seven agronomic traits were collected from corresponding BC2F3 families that were grown at up to six locations in Germany. QTL analyses for yield and yield components were performed using single-marker regression and interval mapping. A total of 57 putative QTLs derived from XX86 were detected, of which 24 (42.1%) were found to have a positive effect from the synthetic wheat XX86. These favourable QTLs were mainly associated with thousand-grain weight and grain weight per ear. Many QTLs for correlated traits were mapped in similar chromosomal regions. The AB-QTL data obtained in the present study are discussed and compared with results from previous QTL analyses. 相似文献
9.
Baohua Wang Xavier Draye Zhimin Zhuang Zhengsheng Zhang Min Liu Edward L. Lubbers Don Jones O. Lloyd May Andrew H. Paterson Peng W. Chee 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(6):1297-1308
Key message
QTLs for fiber length mapped in three generations of advanced backcross populations derived from crossing Gossypium hirsutum and Gossypium mustelinum showed opportunities to improve elite cottons by introgression from wild relatives.Abstract
The molecular basis of cotton fiber length in crosses between Gossypium hirsutum and Gossypium mustelinum was dissected using 21 BC3F2 and 12 corresponding BC3F2:3 and BC3F2:4 families. Sixty-five quantitative trait loci (QTLs) were detected by one-way analysis of variance. The QTL numbers detected for upper-half mean length (UHM), fiber uniformity index (UI), and short fiber content (SFC) were 19, 20, and 26 respectively. Twenty-three of the 65 QTLs could be detected at least twice near adjacent markers in the same family or near the same markers across different families/generations, and 32 QTLs were detected in both one-way variance analyses and mixed model-based composite interval mapping. G. mustelinum alleles increased UHM and UI and decreased SFC for five, one, and one QTLs, respectively. In addition to the main-effect QTLs, 17 epistatic QTLs were detected which helped to elucidate the genetic basis of cotton fiber length. Significant among-family genotypic effects were detected at 18, 16, and 16 loci for UHM, UI, and SFC, respectively. Six, two, and two loci showed genotype?×?family interaction for UHM, UI and SFC, respectively, illustrating complexities that might be faced in introgression of exotic germplasm into cultivated cotton. Co-location of many QTLs for UHM, UI, and SFC accounted for correlations among these traits, and selection of these QTLs may improve the three traits simultaneously. The simple sequence repeat (SSR) markers associated with G. mustelinum QTLs will assist breeders in transferring and maintaining valuable traits from this exotic source during cultivar development.10.
Pillen K Zacharias A Léon J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(8):1591-1601
This paper reports on the first comparative advanced backcross quantitative trait locus (AB-QTL) study in barley. The BC2F2 population H×101 was generated from crossing var. Harry [H; Hordeum vulgare ssp. vulgare (Hv)] with ISR101-23 [101;
H. v. ssp. spontaneum (Hsp)]. The results of the AB-QTL analysis for 13 quantitative traits are presented and, subsequently, compared with the AB-QTL study of the barley cross Apex × ISR101-23 (A×101; Pillen et al., Theor Appl Genet 107:340–352). Both AB populations share the exotic Hsp donor accession ISR101-23. In H×101, 108 putative QTLs (17%) were identified among the 650 marker×trait combinations tested. Altogether 52 (48 %) favorable effects were identified from the exotic parent. At these marker loci, the homozygous Hsp
genotype was associated with an improvement in the trait compared to the homozygous Hv genotype. The percentage of QTLs detected in H×101 was comparable to that in A×101 (17% vs. 15%), however more favorable exotic QTL alleles were located in H×101 than in A×101 (48% vs. 34%). In H×101, the Hsp QTL allele at EBmac0679[4H] was associated with a yield increase of 5.9% averaged across the six environments tested. A comparison of putative QTLs between H×101 and A×101 was based on 26 shared SSR markers. In total, 26% of the putative QTLs could be detected simultaneously in both AB populations. This finding indicates that only a portion of the QTL effects of the donor allele can be transferred from one elite recipient to the next.Communicated by G. WenzelThis work is dedicated to the 65th birthday of Prof. Dr. H.H. Geiger, Department of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Germany. 相似文献
11.
Chee PW Draye X Jiang CX Decanini L Delmonte TA Bredhauer R Smith CW Paterson AH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(4):772-781
A backcross-self population from a cross between Gossypium hirsutum and G. barbadense was used to dissect the molecular basis of genetic variation governing 15 parameters that reflect fiber length. Applying a detailed restriction fragment length polymorphism (RFLP) map to 3,662 BC3F2 plants from 24 independently derived BC3 families, we detected 28, nine, and eight quantitative trait loci (QTLs) for fiber length, length uniformity, and short fiber content, respectively. For eight, six, and two chromosomal regions containing quantitative trait loci (QTLs) for fiber length, length uniformity, and short fiber content (respectively), two-way analysis of variance showed a significant (P<0.001) among-family genotypic effect. A total of 13, two, and four loci showed genotype × family interaction, illustrating some of the complexities that are likely to be faced in introgression of exotic germplasm into the gene pool of cultivated cotton. Co-location of many QTLs for fiber length, length uniformity, and short fiber content accounted for correlations among these traits, while the discovery of many QTLs unique to each trait suggests that maximum genetic gain will require breeding efforts that target each trait (or an index including all three). The availability of DNA markers linked to G. barbadense QTLs identified in this and other studies promise to assist breeders in transferring and maintaining valuable traits from exotic sources during cultivar development.Electronic Supplementary Material Supplementary material is available for this article at 相似文献
12.
Quantitative trait loci for yield and yield components in an Oryza sativa×Oryza rufipogon BC2F2 population evaluated in an upland environment 总被引:1,自引:0,他引:1
P. Moncada C. P. Martínez J. Borrero M. Chatel H. Gauch Jr E. Guimaraes J. Tohme S. R. McCouch 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(1):41-52
An advanced backcross breeding strategy was used to identify quantitative trait loci (QTLs) associated with eight agronomic
traits in a BC2F2 population derived from an interspecific cross between Caiapo, an upland Oryza sativa subsp. japonica rice variety from Brazil, and an accession of Oryza rufipogon from Malaysia. Caiapo is one of the most-widely grown dryland cultivars in Latin America and may be planted as a monoculture
or in a multicropping system with pastures. The objectives of this study were: (1) to determine whether trait-enhancing QTLs
from O. rufipogon would be detected in 274 BC2F2 families grown under the drought-prone, acid soil conditions to which Caiapo was adapted, (2) to compare the performance
with and without pasture competition, and (3) to compare putative QTL-containing regions identified in this study with those
previously reported for populations adapted to irrigated, low-land conditions. Based on analyses of 125 SSLP and RFLP markers
distributed throughout the genome and using single-point, interval, and composite interval mapping, two putative O. rufipogon derived QTLs were detected for yield, 13 for yield components, four for maturity and six for plant height.We conclude that
advanced backcross QTL analysis offers a useful germplasm enhancement strategy for the genetic improvement of cultivars adapted
to stress-prone environments. Although the phenotypic performance of the wild germplasm would not suggest its value as a breeding
parent, it is noteworthy that 56% of the trait-enhancing QTLs identified in this study were derived from O. rufipogon. This figure is similar to the 51% of favorable QTLs derived from the same parent in crosses with a high-yielding hybrid
rice cultivar evaluated under irrigated conditions in a previous study. In conclusion, parallel studies in rice using AB-QTL
analysis provide increasing evidence that certain regions of the rice genome are likely to harbor genes of interest for plant
improvement in multiple environments.
Received: 3 September 1999 / Accepted: 16 May 2000 相似文献
13.
Menkir A Olowolafe MO Ingelbrecht I Fawole I Badu-Apraku B Vroh BI 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(1):90-99
Introduction of exotic maize (Zea mays L.) into adapted tropical germplasm may enhance genetic variability and lead to greater progress from selection. The first objective of this study was to determine if yellow endosperm lines derived from adapted × exotic backcrosses contain exotic alleles that are superior to the recurrent adapted parental line for yield and other agronomic traits in tropical environments. Thirteen exotic yellow maize inbred lines were crossed to an adapted orange line (KUSR) and the F1s were backcrossed to KUSR to generate the first backcrosses. Fifty BC1F4 lines derived from these backcrosses and the recurrent parent were crossed to a common inbred tester (L4001) to form testcrosses, which were evaluated at eight environments in Nigeria. Testcrosses of the BC-derived lines differed significantly for grain yield and other agronomic traits. Only two testcrosses yielded significantly less than L4001 × KUSR, with the best 15 testcrosses producing between 289 and 1,056 kg/ha more grain yield than L4001 × KUSR. The best testcrosses were similar to or better than L4001 × KUSR for other agronomic traits. The second objective of this study was to assess the extent of genetic diversity present among the BC-derived lines. We genotyped 46 BC-derived lines including KUSR and L4001 with 10 AFLP primer pairs and found 491 polymorphic fragments. The average allelic diversity of the lines was 0.30 ± 0.01. The genetic distance of each BC-derived line from KUSR ranged between 0.49 and 0.91. The average genetic distance for all pairs of the BC-derived lines was 0.68 ± 0.004, varying from 0.34 to 0.92. The increased grain yield and genetic diversity observed in these studies provide evidence that exotic germplasm can contribute new alleles to expand the genetic base of tropical maize and develop high-yielding hybrids. 相似文献
14.
Chee P Draye X Jiang CX Decanini L Delmonte TA Bredhauer R Smith CW Paterson AH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(4):757-763
The current study is the first installment of an effort to explore the secondary gene pool for the enhancement of Upland cotton (Gossypium hirsutum L.) germplasm. We developed advanced-generation backcross populations by first crossing G. hirsutum cv. Tamcot 2111 and G. barbadense cv. Pima S6, then independently backcrossing F1 plants to the G. hirsutum parent for three cycles. Genome-wide mapping revealed introgressed alleles at an average of 7.3% of loci in each BC3F1 plant, collectively representing G. barbadense introgression over about 70% of the genome. Twenty-four BC3F1 plants were selfed to generate 24 BC3F2 families of 22–172 plants per family (totaling 2,976 plants), which were field-tested for fiber elongation and genetically mapped. One-way analysis of variance detected 22 non-overlapping quantitative trail loci (QTLs) distributed over 15 different chromosomes. The percentage of variance explained by individual loci ranged from 8% to 28%. Although the G. barbadense parent has lower fiber elongation than the G. hirsutum parent, the G. barbadense allele contributed to increased fiber elongation at 64% of the QTLs. Two-way analysis of variance detected significant (P<0.001) among-family genotype effects and genotype×family interactions in two and eight regions, respectively, suggesting that the phenotypic effects of some introgressed chromosomal segments are dependent upon the presence/absence of other chromosomal segments.Electronic Supplementary Material Supplementary material is available for this article at 相似文献
15.
Yuling Li Yongbin Dong Suzhenniu Niu Dongqun Cui Yanzhao Wang Yanyang Liu Mengguan Wei Xuehui Li 《Molecular breeding : new strategies in plant improvement》2008,21(1):1-14
Specific traits are an important consideration in plant breeding. In popcorn, inferior agronomic traits could be improved
using dent or flint corn backcrossed with popcorn. In this study, we used advanced backcross quantitative trait locus (AB-QTL)
analysis to identify trait-improving QTL alleles from a dent maize inbred Dan232, and compared the detection of QTL in the
BC2S1 population with QTL results using F2:3 families of the same population. Two hundred and twenty BC2S1 families developed from a cross between Dan232 and an elite popcorn inbred N04 were evaluated for nine plant traits in replicated
field trials under two environments. Using composite interval mapping (CIM), a total of 28 significant QTL were detected,
and of these, 23 (82.14%) had favorable alleles contributed by the dent corn parent Dan232. Nine QTL (32.14%) detected in
the BC2S1 population were also located in or near the same chromosome intervals in the F2:3 population. All of the favorable QTL alleles from Dan232 could be used in marker-assisted selection (MAS) to improve the
respective plant traits in popcorn breeding. In addition, their near isogenic lines (QTL-NILs) could be obtained through selfing
or another 1–2 backcross with N04. Also, N04 improved for the studied plant traits could be developed from the BC2S1 families used in this study. This study demonstrated that the AB-QTL method can be applied to identify favorable QTL from
dent corn inbred in popcorn breeding and, once identified, the alleles could be used in marker-assisted selection to improve
the respective plant traits. 相似文献
16.
Testcross performance of rye introgression lines developed by marker-assisted backcrossing using an Iranian accession as donor 总被引:1,自引:0,他引:1
K. C. Falke Z. Sušić P. Wilde H. Wortmann J. Möhring H.-P. Piepho H. H. Geiger T. Miedaner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(7):1225-1238
Introgression libraries facilitate the identification of favorable exotic alleles or genomic regions, which can be exploited
for improving elite breeding material. We evaluated the first two introgression libraries in rye (Secale cereale L.) on the phenotypic and molecular level. Our objectives were to detect candidate introgression lines (pre-ILs) with a better
testcross performance than the recurrent parent and identify donor chromosome segments (DCS) responsible for the improved
performance. We introduced DCS from the self-incompatible heterozygous exotic Iranian primitive rye accession Altevogt 14160
(donor) into the genetic background of the elite inbred line L2053-N (recurrent parent) by marker-assisted backcrossing and
developed 40 BC2S3 lines in each introgression library. Testcross performance for three agronomic and six quality traits was evaluated in replicated
field trials across two testers at five locations over 2 years. The phenotypic effect of the DCS was analyzed for all traits.
The pre-ILs had on average a testcross performance comparable to that of the recurrent parent. Significant (P < 0.05) differences between individual pre-ILs and the recurrent parent were detected for all traits except for heading date.
For more than 60% of the significant (P < 0.05) differences, the pre-ILs were superior to the recurrent parent. For some pre-ILs, specific DCS were identified containing
presumably quantitative trait loci responsible for the superior hybrid performance. Consequently, our study revealed that
the development and employment of introgression libraries offers the opportunity for a targeted increase of genetic diversity
of elite rye material for hybrid performance of agronomically important traits. 相似文献
17.
V TALAMÉ M C SANGUINETI E CHIAPPARINO H BAHRI M BEN SALEM B P FORSTER R P ELLIS S RHOUMA W ZOUMAROU R WAUGH R TUBEROSA 《The Annals of applied biology》2004,144(3):309-319
Advanced backcross QTL (AB-QTL) analysis was deployed to identify allelic variation in wild barley (Hordeum vulgare ssp. spontaneum) of value in the improvement of grain yield and other agronomically important traits in barley (Hordeum vulgare ssp. vulgare) grown under conditions of water deficit in Mediterranean countries. A population of 123 double haploid (DH) lines obtained from BC1F2 plants derived from a cross between Barke (European two-row cultivar) and HOR11508 (wild barley accession) were tested in replicated field trials, under varying conditions of water availability in Italy, Morocco and Tunisia, for seven quantitative traits. Significant QTL effects at one (P 0.001) or more trial sites (P 0.01) were identified for all traits. At 42 (52%) of the 80 putative QTLs identified, the allele increasing a “traits' value” was contributed by H. spontaneum. For example, though the majority (67%) of QTL alleles increasing grain yield were contributed by H. vulgare, H. spontaneum contributed the alleles increasing grain yield at six regions on chromosomes 2H, 3H, 5H and 7H. Among them, two QTLs (associated to Bmac0093 on chromosome 2H and to Bmac0684 on chromosome 5H) were identified in all three locations and had the highest additive effects. The present study shows the validity of deploying AB-QTL analysis for identifying favourable QTL alleles from wild germplasm and indicates its potential as an enhancement strategy for the genetic improvement of cultivars better adapted to drought-prone environments. 相似文献
18.
Robert Vincent J.M. West Marilyn A.L. Inai Shuji Caines Angela Arntzen Lauren Smith Jodee K. St.Clair Dina A. 《Molecular breeding : new strategies in plant improvement》2001,8(3):217-233
Blackmold, caused by the fungus Alternaria alternata, is a major ripe fruit disease of processing tomatoes. Previously, we found blackmold resistance in a wild tomato (Lycopersicon cheesmanii) and quantitative trait loci (QTL) for resistance were mapped in an interspecific population. Five QTLs were selected for introgression from L. cheesmanii into cultivated tomato using marker-assisted selection (MAS). Restriction fragment length polymorphism and PCR-based markers flanking, and within, the chromosomal regions containing QTLs were used for MAS during backcross and selfing generations. BC1 plants heterozygous at the QTLs, and subsequent BC1S1 and BC1S2 lines possessing different homozygous combinations of alleles at the target QTLs, were identified using DNA markers. Field experiments were conducted in 1998 (with 80 marker-selected BC1S2 lines) and 1999 (with 151 marker-selected BC1S2 and BC1S3 lines) at three California locations. Blackmold resistance was assessed during both years, and horticultural traits were evaluated in 1999. The BC1S2 and BC1S3 lines containing L. cheesmanii alleles at the QTLs were associated with a large genetic variance for resistance to blackmold and moderate heritability, suggesting that significant genetic gain may be achieved by selection in this genetic material. L. cheesmanii alleles at three of the five introgressed QTLs showed a significant, positive effect on blackmold resistance. A QTL on chromosome 2 had the largest positive effect on blackmold resistance, alone and in combination with other QTLs, and was also associated with earliness, a positive horticultural trait. The other four QTLs were associated primarily with negative horticultural traits. Fine mapping QTLs using near isogenic lines could help determine if such trait associations are due to linkage drag or pleiotropy. 相似文献
19.
QTL analysis of an advanced backcross of Lycopersicon peruvianum to the cultivated tomato and comparisons with QTLs found in other wild species 总被引:12,自引:0,他引:12
T. M. Fulton T. Beck-Bunn D. Emmatty Y. Eshed J. Lopez V. Petiard J. Uhlig D. Zamir S. D. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):881-894
A BC3 population previously developed from a backcross of Lycopersicon peruvianum, a wild relative of tomato, into the cultivated variety L. esculentum was analyzed for QTLs. Approximately 200 BC4 families were scored for 35 traits in four locations worldwide. One hundred and sixty-six QTLs were detected for 29 of those
traits. For more than half of those 29 traits at least 1 QTL was detected for which the presence of the wild allele was associated
with an agronomically beneficial effect despite the inferior phenotype of the wild parent. Eight QTLs for fruit weight could
be followed through the BC2, BC3, and BC4, generations, supporting the authenticity of these QTLs. Comparisons were made between the QTLs found in this study and those
found in studies involving two other wild species; the results showed that while some of these QTLs can be presumed to be
allelic, most of the QTLs detected in this study are ones not previously discovered.
Received: 9 April 1997 / Accepted: 20 May 1997 相似文献
20.
Fine mapping of three quantitative trait loci for late blight resistance in tomato using near isogenic lines (NILs) and sub-NILs 总被引:16,自引:5,他引:11
Brouwer DJ St Clair DA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(4):628-638
An earlier study identified quantitative trait loci (QTLs) lb4, lb5b, and lb11b for quantitative resistance to Phytophthora infestans (late blight) in a backcross population derived from crossing susceptible cultivated tomato (Lycopersicon esculentum) with resistant L. hirsutum. The QTLs were located in intervals spanning 28–47 cM. Subsequently, near-isogenic lines (NILs) were developed for lb4, lb5b, and lb11b by marker-assisted backcrossing to L. esculentum. Sub-NILs containing overlapping L. hirsutum segments across each QTL region were selected and used to validate the QTL effects, fine-map QTLs, and evaluate potential linkage drag between resistance QTLs and QTLs for horticultural traits. The NILs and sub-NILs were evaluated for disease resistance and eight horticultural traits at three field locations. Resistance QTLs were detected in all three sets of NIL lines, confirming the BC1 mapping results. Lb4 mapped near TG609, and between TG182 and CT194, on chromosome 4, a 6.9-cM interval; lb5b mapped to an 8.8-cM interval between TG69a and TG413 on chromosome 5, with the most likely position near TG23; and lb11b mapped to a 15.1-cM interval on chromosome 11 between TG194 and TG400, with the peak centered between CT182 and TG147. Most QTLs for horticultural traits were identified in intervals adjacent to those containing the late blight resistance QTLs. Fine mapping of these QTLs permits the use of marker-assisted selection for the precise introgression of L. hirsutum segments containing late blight resistance alleles separately from those containing deleterious alleles at horticulturally important QTLs.Electronic Supplementary Material Supplementary material is available in the online version of this article at
Communicated by D.B. Neale 相似文献