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无花果曲霉原生质体形成与再生条件的探讨 总被引:7,自引:1,他引:6
根据正交试验得出无花果曲霉原生质体形成的最佳条件,用1%的混合酶液(0.5%纤维素酶+0.25%蜗牛酶+0.25%溶菌酶)作用无花果曲霉菌体细胞,原生质体产量达3.2×107个·ml-1,渗透压稳定剂为0.6mol·L-1KCl于0.2mol·L-1PO3+4(pH5.8)中,酶解时间和酶解温度分别为3.0h、30℃.比较不同酶解时间、再生稳定剂和碳源等因素对原生质体再生的影响,可确定最佳再生条件,再生率达30%以上. 相似文献
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角质蛋白酶固态发酵工艺及酶解条件的研究 总被引:2,自引:0,他引:2
曲霉A28-8是一株优良的角蛋白酶分泌突变菌株。其最佳固体发酵培养基为:20%羽毛粉,80%麸皮和微量无机氮(每克培养基中加0.5mg);最适产酶条件为:起始pH7.5~8.0,温度28℃~30℃,时间为60~70小时,酶活高达2500KU/g曲;最适酶解条件为pH7.0~9.0,温度45℃~50℃。 相似文献
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米曲霉原生质体融合及杂合二倍体的形成 总被引:19,自引:1,他引:18
采用混合酶液处理纤维素酶高产苗株3042N-2(天冬酰胺缺陷型“Asn-”)及蛋白酶高产菌株3042N-19(蛋氨酸缺陷型“Met-”)的营养菌丝,获得原生质体,以PEG为助融剂进行融合处理,成功地获得了米曲霉(Aspergillusoryzae)原生质体的营养互补融合。将异核体菌落的菌丝转接到合有0.1%樟脑的新鲜MM上,25℃诱导培养7─15天,挑取绿色角变菌落的孢子,将其转接到MM上能迅速生长,经孢 相似文献
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辣椒子叶原生质体分离条件的研究 总被引:5,自引:0,他引:5
以不同基因型的辣椒子叶为供体组织进行辣椒原生质体分离条件的研究,结果表明:幼龄子叶的原生质体产量与活力均高于老龄子叶;酶解过程中酶液渗透压、酶液浓度、酶解时间均对原生质体分离效果产生重要影响。对于辣椒子叶原生质体,最佳分离条件为酶液甘露醇浓度0.5mol/L,纤维素酶Cellulase Onzuka R-10 1.5,果胶酶Macerozyme R-10 0.6%,酶解时间8-10h。不同基因型辣 相似文献
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芽孢杆菌M_(50)产生β甘露聚糖酶的条件研究 总被引:3,自引:0,他引:3
从土壤中分离到9 株产生β甘露聚糖酶的芽孢杆菌( Bacillus sp .) 。Bacillussp . M50250m L三角瓶摇瓶培养试验,以4 % 的魔芋粉为碳源,1-0 % ( NH4)2SO4 为氮源,0-35 %Na2CO3 ,30 ~34 ℃培养60h 产酶达到高峰。酶活力为180 ~200u/m L。100L 罐发酵,在30 ~32 ℃,1∶0 .75vvm 通气量,200r/min 条件下,发酵液酶活力高达330u/m L。酶的最适反应温度和pH 分别为50 ℃和6-0 ,低于50 ℃,pH5 .0 ~7 .0 酶稳定。Fe3+ 、Al3+ 、EDTA、Hg2+ 对酶有抑制作用,而Ba2+ 、Mn2+ 对酶有激活作用。发酵粗酶液对苎麻精干麻精练,显示对精干麻的半纤维素残胶具有降解作用。 相似文献
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灵芝原生质体分离与再生研究 总被引:29,自引:1,他引:28
首次详细,系统地探讨了灵芝原生质体分离与再生的条件,结果表明,用0.6mol/L甘露醇配制成含2%溶壁酶和0.5%崩溃酶的复合醇,在30℃,pH为6.0时酶解3小时,可得到最高的原生质体得率,但考虑到原生质体的再生,以酶解2.5小时为最适。 相似文献
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李耿光 《植物生理与分子生物学学报》1996,(3)
向日葵籽苗下胚轴原生质体,培养在含有BA0.5mg/L,2,4-D0.5mg/L,NAA0.1mg/L和葡萄糖0.55mg/L的改良Kao培养基中,24~28h后,原生质体开始分裂。包埋在琼脂糖0.6%中的原生质体,培养5d后,分裂频率达95%以上。生长旺盛的小愈伤组织转移到含有2ip0.1mg/L,IAA0.01mg/L,腺嘌呤40mg/L和GA30.01mg/L的Thompson液体培养基上13d后,原生质体诱导的少数愈伤组织发生根分化。 相似文献
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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
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The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle. 相似文献
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H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
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Å. Berg 《Bird Study》2013,60(2):153-165
CapsuleThe amount of forest (at local and landscape scales) and occurrence of residual habitats at the local scale are shown to be the major factors influencing bird community composition in farmland–forest landscapes in central Sweden. Aims To investigate the importance of local habitat and landscape structure for breeding birds in farmland–forest landscapes in central Sweden. Methods Breeding birds were censused at 292 points. A detailed habitat mapping was made within 300 m of the points. Within a 300–600 m radius only two major habitats (forests and arable fields) were identified. Results Cluster analyses of bird communities identified three site types that also differed in habitat composition: (i) partially forested sites in forested landscapes; (ii) heterogeneous sites with residual habitats in mosaic landscapes; and (iii) field-dominated farmland sites in open landscapes. A total of 19 of 25 farmland bird species (restricted to farmland or using both farmland and forest) had the highest abundance in farmland sites with mosaics of forest and farmland, while only six farmland species had the highest abundance in field-dominated sites. The bird community changed from being dominated by farmland species to being dominated by forest species (common in forest landscapes without farmland) at small proportions (10–20%) of forest at the local scale. A major difference in habitat composition between heterogeneous and field-dominated sites was the occurrence of different residual habitats (e.g. shrubby areas and seminatural grasslands). These habitats seemed to influence bird community composition more than land-use, despite covering <10% of the area. Seminatural grasslands were important for bird community composition and species-richness, but grazing seemed to be less important. Among different land-use types, cereal crops were the least preferred fields. Set-asides with tall vegetation and short rotation coppices were positively associated with species-richness of farmland birds. Conclusion In general, the composition of the landscape was important for bird community composition, although amount and distribution of forests, occurrence of residual habitats and land-use of fields at the local scale had the strongest influence on bird community composition. The possible implications of these patterns for managing farmland–forest landscapes are discussed. 相似文献
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This work describes the isolation and characterization of twenty-nine compounds from the fruits of Rhus typhina L., including eleven flavonoids (1–11), eleven phenols (12–22), two pentacyclic triterpenes (23–24), two organic acids (25–26), one lumichrome (27), one courmarin (28) and one pyrimidine (29) on the basis of their spectroscopic data. Compounds apigenin (1), daidzein (4), orobol (5), 3′, 5, 5′, 7-tetrahydroxyflavanone (6), naringenin (7), butein (8), (-)-catechin (9), quercetin-3-O-α-L-(3″-O-galloyl)-rhamnoside (11), 2-hydroxybenzoic acid (13), 4-hydroxybenzaldehyde (14), vanillin (15), methyl 3,4-dihydroxybenzoate (16), 3,5-dihydroxybenzamide (18), tyrosol (19), caffeic acid (20), 3-(2,4,6-trihydroxyphenyl)-1-(4-hydroxyphenyl)-propan-1-one (21), phlorizin (22), friedelin (23), oleanolic acid (24), 4,4-dimethyl-heptanedioic acid (25), anthranilic acid (26), lumichrome (27), scoparone (28) and uracil (29) have not been recorded before in this plant. This is the first report on the occurrence of compounds 4–7, 9, 11, 13–14, 16, 18–21, 25–29 from the genus Rhus. Moreover, the chemotaxonomic significance of these isolated compounds was also summarized. 相似文献