从12S rRNA基因第三结构域序列分析探讨蜘蛛若干重要类群的亲缘关系

本文将12S rRNA基因序列分析应用于研究若干重要蜘蛛类群的系统关系,以对传统的分类研究结论进行验证和补充,并且探讨12S rRNA基因序列分析在蜘蛛系统发生研究中的适用性。根据12S rRNA基因第3结构域构建的分子系统树得出结论:1.圆网类(即妖面蛛总科与园蛛总科)并非单系;2.隙蛛与暗蛛较漏斗蛛具有更近的亲缘关系;3.壁钱和拟壁钱并不近缘;4.有筛器类蜘蛛为复系类群;5.12S rRNA基因第3结构域片段对推断近缘科属间的系统发生关系是有效的遗传标记。     

4种蜘蛛染色体研究初报

以早期胚胎细胞为材料,经秋水仙素处理、低渗、固定,制片及染色,制备蜘蛛体细胞染色体标本,并用血细胞染色体制片观察验证,镜检表明,4科4种蜘蛛的体细胞染色体数为:中华涡蛛(Oatonooa sinensis)为17和18,居室拟壁钱(Oecobius cellarioum)为22和24;北国壁钱(Uroctea lesserti)为39和42:大腹圆蛛(Aranea Ventricosus)为49和52。计数结果分析,它们的性别决定染色体机制是:中华涡蛛为XO型,居室拟壁钱为X_1X_2O型,北国壁钱为X_1X_2X_3O型,大腹圆蛛为X_1X_2X_3O型。     

四种蜘蛛的扫描电镜观察

作者以古筛器蛛科的戴氏短栉蛛Ectatosticta davidi(Simon),隐石蛛科的白斑隐蛛Nurscia albofasciata (Strand)、拟壁钱科的居室拟壁钱Oecobius cellariorum(Duges)和园蛛科的十字园蛛Araneus diadematus clerck这四种蜘蛛为材料做了扫描电镜观察。我们在电镜下对若干结构进行不同角度的反复观察并拍摄大量照片,依此进行描述和测量,但本文限于篇幅只能刊出一部分电镜照片。     

一些蜘蛛类群味觉毛的形态、数量和分布

化学通讯是蜘蛛最基础和最普遍的种内及种间通讯方式之一,蜘蛛体表的味觉毛能够接触性地或者近距离地感知环境中的化学物质,但味觉毛的相关研究仅在少数几种蜘蛛中有过报道。我们通过扫描电镜分别对幽灵蛛科(Pholcidae)、弱蛛科(Leptonetidae)、泰莱蛛科(Telemidae)、蟹蛛科(Thomisidae)和球蛛科(Theridiidae)共5科32种蜘蛛味觉毛的形态、数量及分布进行了观察。结果显示:蜘蛛味觉毛一般呈"S"形或弧形;毛根部与体表形成较大角度,末端开口。一般分布在步足的跗节和后跗节,一些种类在步足胫节亦有味觉毛分布。所观察的蜘蛛中绝大部分种类在触肢上未发现味觉毛,仅有2种蟹蛛即角红蟹蛛(Thomisus labefactus)和膨胀微蟹蛛(Lysiteles inflatus)以及1种球蛛即鼬形微姬蛛(Phycosomamustelinum)在触肢上有味觉毛。味觉毛的数量在不同蜘蛛种类中有较大差异,从十几根到上百根不等。蜘蛛味觉毛的形态、数量和分布等特征除了与遗传相关外,亦有可能与其生境和生活方式等有关。     

味觉毛在不同蜘蛛类群中的形态、数量和分布

化学通讯是蜘蛛最基础和最普遍的种内和种间通讯方式之一,蜘蛛体表的味觉毛能够接触性的或者近距离地感知环境中的化学物质,但味觉毛的相关研究仅在少数几种蜘蛛中有过报道。我们通过扫描电镜对分别对幽灵蛛、弱蛛、泰莱蛛、幽灵蛛、蟹蛛和球蛛共5科32种蜘蛛味觉毛的形态、数量和分布进行了观察,结果显示：蜘蛛味觉毛一般呈“S”形或弧形;毛根部与体表形成较大角度,末端开口。一般分布在步足的跗节和后跗节,一些种类在步足胫节亦有味觉毛分布。所观察的蜘蛛中绝在部分种类在触肢上未发现味觉毛,仅有两种蟹蛛 (Thomisus labefactus Lysiteles inflatus) 和一种球蛛 (Phycosoma mustelinum) 在触肢上有味觉毛。味觉毛的数量在不同蜘蛛种类中有较大差异,从十几根到上百根不等。蜘蛛味觉毛的形态、数量和分布等特征除了与遗传相关外,亦有可能与其生境和生活方式等有关。     

花和田蜘蛛群落的研究

在花生田共采集到蜘蛛４１种,隶属于１２科、２７属。游猎型蜘蛛主要是狼蛛科、跳蛛科、猫蛛科和管巢蛛科的种类,所占比例较高,在春花生田为６０．９１～８６．２１％,秋药生田为５０．４０～９０．３６％。结网蜘蛛主要是皿蛛、珠蛛、肖蛸和小型园蛛。在花生的不同生长季节有不同的优势科和优势种。春花生男的优势科是狼蛛科、皿蛛科和猫蛛科,优势种是类水狼蛛、拟环纹豹蛛、脉蜗蛛、食虫沟瘤蛛和斜纹猫蛛;狼蛛科、猫蛛科和球     

农田蜘蛛群落结构及其多样性研究

本文对南宁地区农田自然蜘蛛群落和旱地狼蛛亚群落结构及其多样性进行了研究。 1.农田区蜘蛛群落有14科、41属、80种。主要成分是食虫瘤胸蛛、拟环纹狼蛛、拟水狼蛛、奇异獾蛛和豹蛛属种类,个体数以食虫瘤胸蛛最多。旱地狼蛛亚群落主要成分是奇异獾蛛和豹蛛属种类。 2.稻田区蜘蛛群落多样性指数以田埂杂草群落最高,早稻田群落最低。多样性、均匀度和种类丰富度时间格局是早、晚稻皆随时间序列增加。平均多样性、均匀度和个体数,晚稻均高于早稻。狼蛛更群落多样性和均匀度以草地最高。 3.应用群落比例相似性和聚类分析表明,拟环纹狼蛛与拟水狼蛛和奇异獾蛛与豹蛛属种类是组成当地农田区的水田和旱地生境具有代表性的两个狼蛛亚群落主要成分。 本文试图揭示农田生态系统中的稻田蜘蛛,在不受化学农药干扰下群落结构及其多样性变化,并以狼蛛亚群落为代表,比较农田区内不同生境的群落结构及多样性与生境生态特征的关系,为害虫防治和动物资源的利用提供参考。     

海南稻田蜘蛛群落的结构和动态

报道海南省琼山县稻田蜘蛛群落的物种组成及其优势种群的动态变化。已鉴定的稻田蜘蛛种类有４９种,分属１１分科。食早 瘤蛛Ｕｍｍｅｌｉａｔａ ｉｎｓｅｃｔｉｃｅｐｓ Ｂｏｅｓ．ｅｔ Ｓｔｒ．、草间钻头蛛Ｈｙｌｙｐｈａｎｔｅｓ ｇｒａｍｉｎｉｃｏｌｕｍ（Ｓｕｎｄｅｖａｌｌ）、拟水狼蛛Ｐｉｒａｔａ ｓｕｂｐｉｒａｔｉｃｕｓ（Ｂｏｅｓ．ｅｔ Ｓｔｒ．）、拟环纹豹蛛Ｐａｒｄｏｓａ ｐｓｅｕｄｏａｎｎｕｌａｔａ（     

从核rDNA序列探讨隙蛛亚科Coelotinae的分类地位

隙蛛亚科Coelotinae主要分布于东亚地区,其中我国的已有种类占到全世界种数的一半以上,因此对于我国隙蛛类蜘蛛的研究已经成为世界暗蛛科研究的重点之一。隙蛛亚科属于无筛器类群,于1893年,由Cambridge以隙蛛属为模式属而建立,归属于无筛器的漏斗蛛科。之后,虽然经历了数次修订     

花生田蜘蛛群落的研究

在花生田共采集到蜘蛛41种,隶属于12科、27属。游猎型蜘蛛主要是狼蛛科、跳蛛科、猫蛛科和管巢蛛科的种类,所占比例较高,在春花生田为60.91％～86.21％,秋花生田为50.40％～90.36％。结网蜘蛛主要是皿蛛、球蛛、肖蛸和小型园蛛。在花生的不同生长季节有不同的优势科和优势种。春花生田的优势科是狼蛛科、皿蛛科和猫蛛科,优势种是类水狼蛛、拟环纹豹蛛、脉娲蛛、食虫沟瘤蛛和斜纹猫蛛;狼蛛科、猫蛛科和球蛛科是秋花生田的优势科、脉娲蛛、斜纹猫蛛和八斑鞘蛛为优势种。     

The external morphology of the pseudoscorpion Roncus andreinii

The external morphology of the four postembryonic instars of Roncus andreinii (di Caporiacco, 1925) is described and compared with one of the related R. lubricus L. Koch, 1873 (Gabbutt & Vachon, 1967), with particular emphasis on disposition of the chelal trichobothria in relation to growth.
From the evaluation of characters it is concluded that a few morphometric and chaetotaxic characters (i.e. the length, ratio and shape of pedipalpal articles, the ratio of fingers/pedipalpal hand, the chaetotaxy of sternites II and III) represent good specific discriminants, while the relative position of trichobothria along the chelal axis is nearly the same in both species.
The localization of the growing and non-growing regions along the pedipalpal fingers may be of practical use in the search for new taxonomic characters.     

Agonistic signals received by an arthropod filiform hair allude to the prevalence of near-field sound communication

Arthropod filiform hairs respond to air particle movements and are among the most sensitive animal sensory organs. In many species, they are tuned to detect predators or prey and trigger escape or prey capture behaviours. Here we show for the first time that these hairs also receive intraspecific near-field sound signals in an arachnid. During agonistic encounters, whip spiders (Arachnida, Amblypygi) perform antenniform leg vibration (ALV) displays that have significantly longer duration in contest winners than losers. During an ALV display: (i) the vibrating antenniform leg of the displaying whip spider is positioned close to the trichobothria (filiform hairs) on its opponent's walking legs, (ii) the vibrating antenniform leg can excite these trichobothria via air movements and without direct contact, (iii) the antenniform leg of the displaying whip spider vibrates at a frequency that causes particularly strong, sustained excitation and little adaptation in the trichobothria, and (iv) the duration of an ALV display can be extracted from the response of a trichobothrium. Since filiform hairs are widespread among arthropods, communication via such hairs could be extremely prevalent.     

First description of the male of Cryptothele verrucosa L. Koch, 1872 (Araneae), the type species of the genus

The male of Cryptothele verrucosa L. Koch, 1872, the type species of Cryptothele L. Koch, 1872, known from Fiji and Samoa, is described for the first time. It is compared with the male of C. alluaudi Simon, 1893, the single properly described species of the genus.     

刘承钊教授生前发表的两栖动物新种

本文介绍刘承钊教授生前本人或与合作者描述发表的两栖动物66个新种,分隶2目9科,计有尾目2科9种,无尾目7科57种(名单见附表)。其中,刘承钊和胡淑琴(1961)《中国无尾两栖类》将以下4种列为同物异名:108页:ＡｅｌｕｒｏｐｈｒｙｎｅｂｒｅｖｉｐｅｓＬｉｕ=胸腺猫眼蟾ＡｇｌａｎｄｕｌａｔｕｓＬｉｕ[后改属名,称为胸腺齿突蟾Ｓｃｕｔｉｇｅｒｇｌａｎｄｕｌａｔｕｓ(Ｌｉｕ)]。265页:胡氏树蛙ＲｈａｃｏｐｈｏｒｕｓｈｕｉＬｉｕ=杜氏树蛙ＲｈａｃｏｐｈｏｒｕｓｄｕｇｒｉｔｅｉＤａｖｉｄ[后改属名,称为杜氏泛树蛙Ｐｏｌｙｐｅｄａｔｅｓｄ…     

The Euscorpius tergestinus (C.L. Koch, 1837) complex in Italy: Biometrics of sympatric hidden species (Scorpiones: Euscorpiidae)

This work stems from the results of a recent phylogenetic investigation on the Euscorpius carpathicus species complex from the Italian peninsula (Salomone et al. 2004. Phylogenetic relationships between the sibling species Euscorpius tergestinus and E. sicanus (Scorpiones, Euscorpiidae) as inferred from mitochondrial and nuclear sequence data. In: Proceedings of the16th Congress of Arachnology, August 2-7, 2004, Ghent University, Belgium, 268pp.; Salomone et al. in prep.). Molecular investigation produced interesting and unexpected findings on the scorpion Euscorpius tergestinus (C.L. Koch, 1837). Both nuclear and mitochondrial sequence data provided evidence of substantial genetic differentiation in specimens identified as Euscorpius tergestinus according to recent taxonomical changes (Fet and Soleglad 2002. Morphology analysis supports presence of more than one species in the “Euscorpius carpathicus” complex (Scorpiones: Euscorpiidae). Euscorpius 3, 51pp.). These specimens clearly belong to two well-differentiated evolutionary lineages. Molecular results highlighted the need for a new morphological investigation. The present study undertook the morphological analysis of specimens belonging to both genotypes with the aim of identifying morphological characteristics able to discriminate between the two taxa. The analysis of trichobothria patterns, morphometric ratios, granulation patterns and the observation of the pectinal sensilla confirm the difficulty in distinguishing these two genotypes and the high polymorphism of the subgenus Euscorpius Thorell, 1876. The length of pedipalp segments and dorsal patellar spurs (DPS), as well as femur leg granulation, are the main diagnostic characters; other ratios together with body color also help to distinguish the different genotypes. This study confirms the presence in Italy of two different cryptic species belonging to the “Euscorpius tergestinus” complex. Euscorpius tergestinus is a reddish, slender euscorpiid with a large dorsal patellar spine (DPS). A darker and generally squat phenotype with a short DPS, which corresponds to Euscorpius carpathicus concinnus sensu Caporiacco (1950), is elevated to the species level: Euscorpius concinnus (C.L. Koch, 1837). These two species are sympatric in several Italian regions, and their distribution pattern is possibly determined by intraguild predaction interaction.     

A survey of oonopid spiders in Taiwan with descriptions of three new species

The oonopid spiders of Taiwan are surveyed. Seven genera and 13 species are recognized, including 3 new species: Ischnothyreus kentingensis sp. n., Xyphinus hwangi sp. n. and Xestaspis shoushanensis sp. n. Seven species are newly recorded from this region: Brignolia parumpunctata (Simon, 1893), Opopaea apicalis s (Simon, 1893), Opopaea cornuta Yin & Wang, 1984, Opopaea deserticola Simon, 1891, Orchestina sinensis Xu, 1987, Pseudotriaeris karschi (Bösenberg & Strand, 1906) and Xestaspis loricata (L. Koch, 1873).     

Vestured pits and vestured vessel member walls in some Indian dicotyledonous woods

NAIR, M. N. B. AND MOHAN RAM, H. Y., 1989. Vestured pits and vestured vessel member walls in some Indian dicotyledonous woods. The woods of 144 taxa belonging to 38 families of angiosperms were examined for vestured pits and vestured vessel member walls using scanning electron microscopy. Vestured pits are present in 66 taxa (belonging to ten families) and vestured vessel member walls occur in only six taxa (belonging to three families). In Ehretiaceae and Euphorbiaceae vestures are present only in certain vessel members. In Wrighlia tinctoria , perforation plates containing vestures have been observed in addition to the presence of vestured pits. A classification of vestured pits based on their morphology and distribution is proposed by us. In all the types of vestured pits, vestures are present on the margin of the outer pit aperture or on the pit chamber wall. Occasionally, they are present in the pit canal, on the margin and in the vicinity of the inner pit aperture and rarely over the inner walls of the vessel members. The functions of vestured pits are not clear, although several suggestions are made. Whether or not these structures affect wood processing is not presently understood. It appears that vestured pits and vestured vessel member walls have diagnostic rather than phylogenetic value.