超声刀在425例妇科腹腔镜手术中的应用分析

目的:探讨超声刀在妇科腹腔镜手术中的应用方法和特点;方法:回顾我院2006年1月～2010年3月因妇科疾病住院使用超声刀行腹腔镜手术425例病例构成和手术效果.结果:全部病例均顺利完成手术,无中转开腹,其中腹腔镜下完全子宫切除术36例,腹腔镜辅助阴式子宫切除术89例,子宫次全切切除术145例,子宫肌瘤剔除手术68例,附件手术87例.子宫手术时间平均100 min,术中出血平均120 ml,附件手术时间平均55 min,出血平均60 ml,术后平均住院4天,未发生术中及术后严重并发症.结论:应用超声刀进行妇科腹腔镜手术是安全可行的,并具有良好效果.     

腹腔镜辅助下阴式子宫切除术与阴式子宫全切除术治疗子宫良性病变的临床比较

目的:探究阴式子宫全切除术与腹腔镜辅助下阴式子宫切除术治疗子宫良性病变的临床效果。方法:选择2011年10月~2013年9月我院收治的子宫良性病变的患者120例,其中行腹腔镜辅助下阴式子宫切除术80例(研究组)和行阴式子全宫切除术40例(对照组),观察并分析两组的临床指标及手术并发症。结果:研究组住院费用明显高于对照组(P0.05),手术时间、术中出血量及住院时间均低于对照组(P0.05);研究组无邻近脏器损伤,对照组输尿管损伤2例(5.00%),研究组患者出现手术损伤率明显低于对照组,差异存在统计学差异(x2=3.968,P=0.046);对两组患者术后随访1年,两组患者均无出现再手术情况;两组患者术后阴道残端均愈合较好,未出现阴道残端漏,患者无大小便困难及其他不适。结论:腹腔镜辅助下阴式子宫切除术治疗子宫良性病变疗效优于阴式子宫全切术,其手术时间、术中出血量及住院时间均较低,创伤小,更有利于患者恢复健康,在临床上值得应用推广。     

基层医院腹腔镜辅助阴式子宫全切除术临床分析

目的探讨基层医院腹腔镜辅助阴式子宫全切除术的治疗效果。方法回顾性分析2013年6月~2015年12月在我院行腹腔镜辅助阴式全子宫切除术患者47例的临床资料。结果 46例患者手术成功,成功率97.87%;1例因肌瘤较大中转开腹切除术。本组腹腔镜辅助阴式子宫切除手术时间平均104(81~195)min,出血量平均120(90~140)ml,术后住院时间4~5天。结论腹腔镜辅助阴式全子宫切除术具有创伤小,术后恢复快,安全性高,易于操作等优点,尤其适合在基层医院推广应用。     

腹腔镜下子宫肌瘤剥出术31例临床研究

目的:探讨腹腔镜下子宫肌瘤剥出术的临床效果及应用价值。方法:收集我院2009年4月至2010年5月行腹腔镜下肌瘤剥出术31例的临床资料,并与同期25例传统开腹肌瘤剥出术做对照,比较两组患者手术时间、术中出血量、术后排气时间、术后并发症发生率及术后平均住院日。结果:实验组术中出血量、术后体温恢复正常时间、术后排气时间、术后并发症发生率及术后平均住院日与对照组比较,差异有统计学意义(P<0.01或P<0.05)。结论:腹腔镜下肌瘤剥出术不仅优于传统开腹术,也优于传统开腹肌瘤剥出术手术,选择适应证和掌握熟练的操作技巧是手术成功的关键。.     

宫腔镜与腹腔镜手术剔除子宫肌瘤临床疗效观察

目的探讨宫腔镜与腹腔镜手术剔除子宫肌瘤的临床疗效。方法将我院收治的118例子宫肌瘤患者随机分为对照组与观察组,对照组实施腹腔镜子宫肌瘤剔除术,观察组实施宫腔镜子宫肌瘤电切术治疗,比较两组围手术期指标、术后子宫肌层愈合情况以及术后妊娠情况。结果观察组的手术时间、术中出血量、术后肌层完全愈合率以及首次妊娠距手术时间均显著优于对照组,差异有统计学意义(P0.05);两组术中转开腹率、术后发热率、月经改善率以及术后妊娠率比较,差异无显著性意义(P0.05)。结论宫腔镜与腹腔镜手术治疗子宫肌瘤均安全可靠,但宫腔镜术中出血量更少、术后恢复更迅速,是有生育要求患者的首选术式。     

腹腔镜与传统开腹手术治疗老年胃十二指肠穿孔患者的效果比较

目的观察比较腹腔镜与传统开腹手术治疗老年胃十二指肠穿孔的临床效果。方法选择2013年7月-2014年12月我院收治的老年胃十二指肠穿孔患者116例,随机分为腹腔镜组与开腹组,每组58例,比较两组的临床效果。结果腹腔镜组术中出血量、术后肛门排气时间、下床活动时间、术后体温升高率以及住院时间均明显少于开腹组,差异有统计学意义(P0.05)。结论采用腹腔镜下治疗老年胃十二指肠穿孔患者对患者的损伤较小,患者术后恢复快,值得推广应用。     

传统开腹手术与腹腔镜手术治疗子宫肌瘤的效果分析

目的探讨传统开腹手术与腹腔镜手术治疗子宫肌瘤的临床疗效。方法选择2013年2月~2015年11月在我院接受治疗的子宫肌瘤患者140例为研究对象,按患者自愿原则分为对照组和观察组各70例,对照组采用传统开腹手术治疗,观察组采用腹腔镜手术治疗,并对两组患者手术时间、术中出血量、术后感染情况和住院时间进行比较。结果观察组在手术时间、术中出血量、术后感染率及住院时间等方面均优于对照组,差异具有显著统计学意义(P0.05)。结论腹腔镜下子宫肌瘤切除术与传统开腹手术比较,具有出血量少、住院时间短、恢复快、并发症少等优点,值得临床推广应用。     

腹腔镜下子宫肌瘤剥出术31例临床研究

目的：探讨腹腔镜下子宫肌瘤剥出术的临床效果及应用价值。方法：收集我院2009年4月至2010年5月行腹腔镜下肌瘤剥出术31例的临床资料,并与同期25例传统开腹肌瘤剥出术做对照,比较两组患者手术时间、术中出血量、术后排气时间、术后并发症发生率及术后平均住院日。结果：实验组术中出血量、术后体温恢复正常时间、术后排气时间、术后并发症发生率及术后平均住院日与对照组比较,差异有统计学意义（P〈0．01或P〈0．05）。结论：腹腔镜下肌瘤剥出术不仅优于传统开腹术,也优于传统开腹肌瘤剥出术手术,选择适应证和掌握熟练的操作技巧是手术成功的关键。     

腹腔镜联合胆道镜治疗胆囊息肉合并胆囊结石的临床效果

目的:探讨腹腔镜联合胆道镜治疗胆囊息肉并发胆囊结石的临床成效及并发症。方法:选取我院肝胆外科收治的胆囊息肉并发胆囊结石患者92例,随机分为两组。其中对照组46例,行传统开腹手术治疗;实验组46例,行腹腔镜联合胆道镜保胆手术治疗。对比两组患者手术平均时间、术中出血量、术后排气时间、术后下床活动时间、平均住院时间以及术后并发症发生率。结果:1实验组手术时间明显长于对照组,实验组患者术中出血量、术后排气时间、术后下床活动时间、平均住院时间明显较对照组明显缩短,差异有统计学意义(P0.05);2实验组术后并发症发生率(6.52%)明显低于对照组(16.67%),差异有统计学意义(P0.05)。结论:腹腔镜联合胆道镜治疗胆囊息肉并发胆结石能够明显减少患者术中出血量,缩短术后排气时间、术后下床活动时间、平均住院时间,降低术后并发症发生率,有利于提高临床疗效,改善预后,对临床具有指导意义。     

腹腔镜结肠癌根治术与传统开腹结肠癌根治术的临床对比分析

目的:探讨腹腔镜结肠癌根治术与传统开腹结肠癌根治术疗效的差别。方法:随机将86例结肠癌患者分为两组,各43例。腹腔镜组采用腹腔镜结肠癌根治术,传统开腹组采用开腹结肠癌根治术,观察两组患者手术情况及术后并发症情况。结果:腹腔镜组手术时间、淋巴结清扫数与传统开腹组比较无显著差异(P0.05);腹腔镜组术中出血量少于传统开腹组、住院时间短于传统开腹组(P0.05);腹腔镜组排气时间短于传统开腹组(P0.01),两组并发症发生率差异有统计学意义(P0.01)。结论:腹腔镜结肠癌根治术具有术中出血量少、创伤小、术后恢复快、住院时间明显缩短等特点,可达到传统开腹手术的效果,值得广泛应用。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细