共查询到20条相似文献,搜索用时 250 毫秒
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Polesskaya A Duquet A Naguibneva I Weise C Vervisch A Bengal E Hucho F Robin P Harel-Bellan A 《The Journal of biological chemistry》2000,275(44):34359-34364
The myogenic protein MyoD requires two nuclear histone acetyltransferases, CREB-binding protein (CBP)/p300 and PCAF, to transactivate muscle promoters. MyoD is acetylated by PCAF in vitro, which seems to increase its affinity for DNA. We here show that MyoD is constitutively acetylated in muscle cells. In vitro, MyoD is acetylated both by CBP/p300 and by PCAF on two lysines located at the boundary of the DNA binding domain. MyoD acetylation by CBP/p300 (as well as by PCAF) increases its activity on a muscle-specific promoter, as assessed by microinjection experiments. MyoD mutants that cannot be acetylated in vitro are not activated in the functional assay. Our results provide direct evidence that MyoD acetylation functionally activates the protein and show that both PCAF and CBP/p300 are candidate enzymes for MyoD acetylation in vivo. 相似文献
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The basal level of the tumor suppressor p53 is regulated by MDM2-mediated ubiquitination at specific lysines, which leads to p53 nuclear export and degradation. Upon p53 activation, however, these lysines become acetylated by p300/CREB-binding protein. Here we have reported an unexpected finding that p300-mediated acetylation also regulates p53 subcellular localization and can promote cytoplasmic localization of p53. This activity is independent of MDM2 but requires a p53 nuclear export signal and acetylation of multiple lysines by p300. Mechanistically, we showed that conversion of a minimal four of these lysines to alanines but not arginines mimics p300-mediated p53 nuclear export, and these lysine-neutralizing mutations effectively prevent p53 tetramerization, thus exposing the oligomerization-regulated nuclear export signal. Our study suggested a threshold mechanism whereby the degree of acetylation regulates p53 nucleus-cytoplasm trafficking by neutralizing a lysine-dependent charge patch, which in turn, controls oligomerization-dependent p53 nuclear export. 相似文献
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Guo S Cichy SB He X Yang Q Ragland M Ghosh AK Johnson PF Unterman TG 《The Journal of biological chemistry》2001,276(11):8516-8523
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Acetylation of androgen receptor enhances coactivator binding and promotes prostate cancer cell growth 总被引:5,自引:0,他引:5 下载免费PDF全文
Fu M Rao M Wang C Sakamaki T Wang J Di Vizio D Zhang X Albanese C Balk S Chang C Fan S Rosen E Palvimo JJ Jänne OA Muratoglu S Avantaggiati ML Pestell RG 《Molecular and cellular biology》2003,23(23):8563-8575
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