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1.
Choong Hyun Lee Jung Hoon Choi Ki-Yeon Yoo Ok Kyu Park In Koo Hwang Sang Guan You Boo-Yong Lee Il-Jun Kang Moo-Ho Won 《Cellular and molecular neurobiology》2010,30(2):255-263
Melatonin exerts many physiological functions via its G protein-coupled receptors. In the present study, we investigated age-related
changes in MT2 melatonin receptor immunoreactivity and its levels in the gerbil hippocampus during normal aging. In the postnatal
month 1 (PM 1) group, MT2 immunoreaction was well observed in neurons in all subregions of the gerbil hippocampus. In the
PM 3 and 6 groups, MT2 immunoreactivity in neurons was decreased compared to that in the PM 1 group. Thereafter, MT2 immunoreactivity
in neurons was increased. In the PM 18 and 24 groups, MT2 immunoreactivity in neurons was strong in all subregions of the
gerbil hippocampus. In addition, the number of MT2 immunoreactive cells was lowest at PM 3 and highest at PM 24. From western
blot analysis, age-dependent change pattern in MT2 level in the gerbil hippocampus was similar to the immunohistochemical
result. These results indicate that MT2 immunoreactivity and levels are altered in the gerbil hippocampus during normal aging;
lowest at young adult stage and highest at aged stage. 相似文献
2.
Choong Hyun Lee In Koo Hwang Ki-Yeon Yoo Jung Hoon Choi Ok Kyu Park Jae-Chul Lee Young-Gil Jeong In Se Lee Moo-Ho Won 《Cellular and molecular neurobiology》2009,29(5):665-672
The hippocampus is associated with learning and memory function and shows neurochemical changes in aging processes. Calbindin
D-28k (CB) binds calcium ion with a fast association rate. We examined age-related changes in CB immunoreactivity and its
protein level in the gerbil hippocampus during normal aging. In the hippocampal CA1 region (CA1) and CA2, CB immunoreaction
was found in some neurons in the stratum pyramidale (SP) at postnatal month 1 (PM 1). CB immunoreactivity in neurons was markedly
increased at PM 3. Thereafter, CB immunoreactivity was decreased with time: CB-immunoreactive (+) neurons were fewest at PM 24. In the CA3, a few CB+ neurons were found only in the SP at PM 1 and in the stratum radiatum at PM 18 and 24. In addition, mossy fibers were stained
with CB at PM 1. CB immunoreactivity in mossy fibers was markedly increased at PM 3, thereafter it was decreased with time.
In the dentate gyrus, many granule cells (GC) in the granule cell layer were stained with CB at PM 1. CB immunoreactivity
in GC was markedly increased at PM 3, thereafter CB immunoreactivity was decreased with time. In Western blot analysis, CB
protein level in the gerbil hippocampus was highest at PM 3, thereafter CB protein levels were decreased with time. This result
indicates that CB in the gerbil hippocampus is abundant at PM 3 and is decreased with age. 相似文献
3.
Lee CH Yoo KY Ryu PD Park JH Choi JH Kim S Hwang IK Kim YM Won MH 《Cellular and molecular neurobiology》2011,31(3):345-350
In this study, we investigated age-related changes in glucagon-like peptide-1 receptor (GLP-1R) immunoreactivity and its protein
levels in the gerbil hippocampus during normal aging. In the postnatal month 3 (PM 3) group, GLP-1R immunoreaction was well
observed in neurons, especially pyramidal and non-pyramidal cells in the hippocampus proper, and granule and polymorphic cells
in the dentate gyrus. In the hippocampus proper, GLP-1R immunoreactivity in neurons was maintained until PM 24. In the dentate
gyrus, however, GLP-1R immunoreactivity in granule cells, not polymorphic cells, was hardly detected from PM 6. Western blot
analysis also showed that age-dependent change patterns in GLP-1R protein levels in the gerbil hippocampus were similar to
the immunohistochemical changes. These results indicate that GLP-1R immunoreactivity was markedly decreased in dentate granule
cells from PM 6, showing that GLP-1R immunoreactivity and its protein levels were decreased in the adult and aged gerbil hippocampus. 相似文献
4.
Age-Dependent Changes in Calretinin Immunoreactivity and its Protein Level in the Gerbil Hippocampus
Choong Hyun Lee In Koo Hwang Jung Hoon Choi Ki-Yeon Yoo Ok Kyu Park Sung-Oh Huh Yun Lyul Lee Hyung-Cheul Shin Moo-Ho Won 《Neurochemical research》2010,35(1):122-129
Calretinin (CR)-immunoreactive interneurons are well known as the interneuron specific interneurons in the hippocampus. CR-immunoreactive
neurons form cellular network and regulate the activity of other GABAergic inhibitory interneurons in the hippocampus. In
the present study, we investigated age-related changes in CR-immunoreactive neurons and protein levels in the gerbil hippocampus
during normal aging. In all subregions of the gerbil hippocampus, the number of CR-immunoreactive neurons was significantly
decreased in the postnatal month 6 (PM 6) group compared to that in the PM 1 group. Thereafter, CR-immunoreactive neurons
were decreased with age. In addition, the number of CR-immunoreactive cells in the subgranular zone were significantly decreased
in the PM 6 group. We also observed that CR protein levels were decreased gradually with age. These results indicate that
both CR immunoreactivity and its protein level were decreased with age in the gerbil hippocampus during normal aging. 相似文献
5.
Choong Hyun Lee Ki-Yeon Yoo Ok Kyu Park Jung Hoon Choi Il-Jun Kang Eunjoo Bae Sung Koo Kim In Koo Hwang Moo-Ho Won 《Molecules and cells》2010,29(4):373-378
Phosphorylated extracellular signal-regulated kinase (pERK) mediates neuronal synaptic plasticity, long-term potentiation,
and learning and memory in the hippocampus. In this study, we examined pERK1/2 immunoreactivity and its protein level in the
gerbil hippocampus at various ages. In the postnatal month 1 (PM 1) group, very weak pERK1/2 immunoreactivity was detected
in the hippocampus. In the CA1 region, pERK1/2 immunoreactivity was considerably increased in the stratum pyramidale in the
PM 6 group. Thereafter, pERK1/2 immunoreactivity was decreased. In the CA2/3 region, pERK1/2 immunoreactivity increased in
an age-dependent manner until PM 12. Thereafter, numbers of pERK1/2-immunoreactive neurons were decreased. However, in the
mossy fiber zone, pERK1/2 immunostaining became stronger with age. In the dentate gyrus, a few pERK1/2-immunoreactive cells
were observed until PM 12. In the PM 18 and 24 groups, numbers of pERK1/2-immunoreactive cells were increased, especially
in the polymorphic layer. In Western blot analysis, pERK1/2 level in the gerbil hippocampus was increased with age. These
results indicate that total pERK1/2 levels are increased in the hippocampus with age. However pERK1/2 immunoreactivity in
subregions of the gerbil hippocampus was changed with different pattern during normal aging. 相似文献
6.
Hwang IK Yoo KY Yi SS Kwon YG Ahn YK Seong JK Lee IS Yoon YS Won MH 《Neurochemical research》2008,33(5):867-872
In the present study, we investigated age-related changes of newborn neurons in the gerbil dentate gyrus using doublecortin
(DCX), a marker of neuronal progenitors which differentiate into neurons in the brain. In the postnatal month 1 (PM 1) group,
DCX immunoreactivity was detected in the subgranular zone of the dentate gyrus, but DCX immunoreactive neurons did not have
fully developed processes. Thereafter, DCX immunoreactivity and its protein levels in the dentate gyrus were found to decrease
with age. Between PM 3 and PM 18, DCX immunoreactive neuronal progenitors showed well-developed processes which projected
to the granular layer of the dentate gyrus, but at PM 24, a few DCX immunoreactive neuronal progenitors were detected in the
subgranular zone of the dentate gyrus. DCX protein level in the dentate gyrus at PM 1 was high, thereafter levels of DCX were
decreased with time. The authors suggest that a decrease of DCX immunoreactivity and its protein level with age may be associated
with aging processes in the hippocampal dentate gyrus. 相似文献
7.
Choong Hyun Lee Ki-Yeon Yoo Jung Hoon Choi Ok Kyu Park In Koo Hwang Il-Jun Kang Moo-Ho Won 《Neurochemical research》2010,35(1):99-106
Cyclooxygenases-2 (COX-2) is not only related to inflammation but also plays critical roles in brain development and synaptic
signaling. In the present study, we investigated age-related changes in COX-2 immunoreactivity and protein levels in the gerbil
hippocampus. In the hippocampal CA1 region (CA1) and dentate gyrus (DG), weak COX-2 immunoreactivity was observed at postnatal
month 1 (PM 1), and COX-2 immunoreactivity was markedly increased at PM 18 and 24. In the CA2/3, COX-2 immunoreactivity was
strong at PM 1. COX-2 immunoreactivities in the PM 3, 6 and 12 groups were decreased compared to that in the PM 1 group, and
it was increased at PM 18 and 24. In addition, age-related changes in COX-2 levels were similar with immunohistochemical results
in the CA2/3. These results suggest that COX-2 immunoreactivity and levels were high in the hippocampus of aged gerbils. 相似文献
8.
Hippocampal interneurons are local circuit neurons which are responsible for inhibitory activity in the hippocampus. Parvalbumin
(PV) is one of useful markers for GABAergic interneurons, not for principle cells, in the hippocampus. In the present study,
we investigated age-related changes in PV immunoreactive neurons and protein levels in the gerbil hippocampus during normal
aging. PV immunoreactive neurons were detected in all hippocampal subregions of all groups. PV immunoreactive neurons, which
innervated principal neurons, were non-pyramidal neurons in the hippocampal CA1-3 regions, and were polymorphic neurons in
the dentate gyrus. In the hippocampal CA1 region, the number of PV immunoreactive neurons was significantly reduced in the
postnatal month 3 (PM 3) group, which was sustained by PM 18, and, at PM 24, the number of PV immunoreactive neurons was significantly
decreased. In the CA2/3 region and dentate gyrus, the number of PV immunoreactive neurons was significantly decreased at PM
6: Thereafter, the number of PV immunoreactive neurons was sustained until PM 24. In addition, changes in PV protein levels
in the gerbil hippocampus were similar to immunohistochemical changes during normal aging: PV protein levels were significantly
decreased with age by PM 6: Thereafter, PV protein levels were sustained by PM 24. These results suggest that PV immunoreactive
interneurons were decreased in the hippocampus with age in gerbils. 相似文献
9.
Chan Woo Park Ki-Yeon Yoo In Koo Hwang Jung Hoon Choi Choong Hyun Lee Ok Kyu Park Jun Hwi Cho Yun Lyul Lee Hyung-Cheul Shin Moo-Ho Won 《Neurochemical research》2009,34(12):2154-2162
The insulin receptor has been reported to be associated with memory formation via the hippocampus. In this study, we observed
age-related changes in the insulin receptor β immunoreactivity and its protein levels in the hippocampus of gerbils of various
ages in order to identify the correlation between the insulin receptor β and aging processes in the hippocampus. Insulin receptor
β immunoreactivity was mainly detected in the molecular and polymorphic layers of the dentate gyrus, and in mossy fibers,
Schaffer collaterals, alveus and stratum lacunosum-moleculare of the hippocampus proper (CA1-3) of gerbils at postnatal month
1 (PM 1). Insulin receptor β immunoreactivity decreased with age in all of these structures, except for the alveus. Reduction
of the insulin receptor β immunoreactivity was prominent in the molecular layer of the dentate gyrus at PM 6 and in the stratum
lacunosum-moleculare of the CA1 region at PM 12, while insulin receptor β immunoreactivity was decreased in other regions
in the PM 18 groups. In addition, insulin receptor β protein level in the whole hippocampus was slightly increased at PM 3,
and it decreased in an age-dependent manner from PM 6 to PM 24. These reductions of the insulin receptor β in the hippocampus
may be associated with age-related memory deficits in gerbils. 相似文献
10.
Ki-Yeon Yoo Ok Kyu Park Jiatian Yu Bingchun Yan Hua Li Choong Hyun Lee Jung Hoon Choi Dae Won Kim In Koo Hwang Moo-Ho Won 《Cellular and molecular neurobiology》2009,29(3):413-421
Oxidative stress is one of predisposing factors to age-related neurodegeneration in the brain. In particular, thiol-containing
groups are susceptible to oxidative stress, which induces the formation of the disulfide bond and/or hyperoxidized form of
thiol-containing proteins. We observed the protein thiol levels in the hippocampal homogenates and also investigated changes
in hyperoxidized form of peroxiredoxin (Prx–SO3) immunoreactivity and proteins levels in the gerbil hippocampal subregions during normal aging. Levels of total thiol, non-protein
thiol, and protein thiol were decreased in the hippocampal homogenates with age. At post-natal month 1 (PM 1), pyramidal and
non-pyramidal cells in the hippocampal CA1 region (CA1) showed Prx–SO3 immunoreactivity. Prx–SO3 immunoreactivity in the cells was decreased by PM 12, thereafter, Prx–SO3 immunoreactivity in the cells increased again with age. In the CA2/3, Prx–SO3 immunoreactivity in pyramidal cells was not significantly changed; however, the immunoreactivity in pyramidal cells was very
low at PM 12. Prx–SO3 immunoreactivity in the dentate gyrus (DG) was distinctly changed during aging. At PM 1, Prx–SO3 immunoreactivity in granule and polymorphic cells was weak and strong, respectively. The immunoreactivity in the neurons
was decreased with age, not shown in any neurons at PM 12. Thereafter, Prx–SO3 immunoreactivity increased again with age. In addition, Prx–SO3 protein level in the hippocampus was lowest at PM 12. These results suggest that thiol-containing proteins are changed during
aging and Prx–SO3 immunoreactivity was different according to cells in the hippocampal subregion during aging. 相似文献
11.
Age-dependent studies on oligodendrocytes, which are the myelinating cells in the central nervous system, have been relatively
less investigated. We examined age-dependent changes in Rip immunoreactivity and its protein level in the gerbil hippocampus
during normal aging using immunohistochemistry and Western blot analysis with Rip antibody, an oligodendrocyte marker. Rip
immunoreactivity and its protein level in the hippocampal CA1 region significantly increased at postnatal month 3 (PM 3).
Thereafter, they decreased in the hippocampal CA1 region with age. At PM 24, Rip immunoreactive processes in the hippocampal
CA1 region markedly decreased in the stratum radiatum. In the hippocampal CA2/3 region and dentate gyrus, the pattern of changes
in Rip immunoreactivity and its protein level was similar to those in the hippocampal CA1 region; however, no significant
changes were found in the CA2/3 region and dentate gyrus at various age stages. These results indicate that Rip immunoreactivity
and protein level in the hippocampal CA1 region decreases significantly at PM 24 compared to the CA2/3 region and dentate
gyrus. 相似文献
12.
Hwang IK Yoo KY Li H Choi JH Lee CH Chung DW Lee IS Kim DW Choi SY Won MH 《Neurochemical research》2008,33(5):938-944
Adrenalectomy (ADX) has been useful for a good in vivo model for apoptosis in the hippocampus by the absence of corticosteroids
following ADX. In some neurodegenerative diseases, GABAergic neurons are more resistant to neuronal damage as compared with
glutamatergic neurons. In the present study, we observed chronological changes in three GABA degradation enzymes, e.g., GABA
transaminase (GABA-T), succinic semialdehyde dehydrogenase (SSADH) and succinic semialdehyde reductase (SSAR) immunoreactivity
and protein levels in the gerbil hippocampal CA1 region after ADX. Changes in their immunoreactivities were distinct in the
stratum pyramidale of the CA1 region. GABA-T immunoreactivity and protein level were significantly increased in the CA1 region
3 h after ADX, in contrast, SSAR and SSADH immunoreactivity and protein level were increased 12 h and 3–12 h, respectively,
after ADX. These results suggest that the increases of GABA-T, SSADH and SSAR immunoreactivity and protein levels in the hippocampal
CA1 region in ADX gerbils may be associated with the control of GABA levels in this region. 相似文献
13.
Choi JH Hwang IK Lee CH Chung DW Yoo KY Li H Won MH Seong JK Yoon YS Lee IS 《Neurochemical research》2008,33(3):562-568
Corticosteroids are important factors in the maintenance of homeostasis in the brain. They are regulated via the interaction
with two corticosteroid receptor systems—the mineralocorticoid (MR) and glucocorticoid receptor (GR). In the present study,
we observed age-related changes in serum cortisol levels, and immunoreactivities and protein levels of MR and GR in the hippocampal
CA1 region and dentate gyrus. The serum cortisol levels were significantly high (about twofold) in the aged group compared
to that in the adult group. In the adult dog (2–3 years old), MR and GR immunoreactivity was detected in neurons in the pyramidal
layer of the CA1 region, and in the granular and multiform layers of the dentate gyrus. In the aged dog (10–12 years old),
MR immunoreactivity in the CA1 region was significantly decreased, especially, in the dentate multiform layer. In contrast,
GR immunoreactivity in the aged dog was slightly decreased in the CA1 region and dentate gyrus. In the Western blot analysis,
MR protein level in the aged dog was significantly lower compared to that of the adult dog; GR protein level in the aged dog
was not significantly decreased. This result indicates that the reduction of MR immunoreactivity and protein level in the
hippocampus of the aged dog may be associated with neural dysfunction in the aged hippocampus. 相似文献
14.
15.
Changes in the expression of calbindin D-28k in the gerbil hippocampus following seizure 总被引:2,自引:0,他引:2
Hwang IK Nam YS Chung DW Lee HS Yoon YS Yoo KY Kang TC Lee IS Won MH 《Neurochemistry international》2004,44(3):145-152
Previous studies have reported that calbindin D-28k (CB), a calcium-binding protein, containing neurons in the hippocampus play an important role in hippocampal excitability in epilepsy, because CB modulates the free calcium ion during seizure. Hence, in the present study, we investigated changes of CB expression in the hippocampus and its association in the Mongolian gerbil to identify roles of CB in epileptogenesis. CB immunoreactivity in the hippocampus was significantly lower in the pre-seizure group of seizure sensitive (SS) gerbils as compared with those seen in the seizure resistant (SR) gerbils. The distribution of CB immunoreactivity in the hippocampus showed significant difference after seizure on-set in SS gerbils. CB immunoreactivity in the hippocampal CA1, CA2 areas, and subiculum was lowest at 3h after seizure on-set; thereafter, the immunoreactivity became to increase to 12h after seizure on-set. Mossy fibers, Schaffer collaterals and dentate granule cells showed the highest CB immunoreactivity at 3h after seizure on-set; thereafter, the immunoreactivity became to decrease. In the case of the intrinsic and output connections of the hippocampus, a rapid decrease of CB serves an inhibitory function, which regulates the seizure activity and output signals from the hippocampus. 相似文献
16.
Park JH Lee CH Yan BC Ahn JH Lee YJ Park CW Cho JH Choi SY Lee YL Won MH Lee HY 《Neurochemical research》2012,37(7):1428-1435
Ribosomal protein S3 (rpS3), a multi-functional protein, has been known to participate in DNA repair mechanism. In this study, we investigated changes in rpS3 immunoreactivity and its protein levels in the sub-regions of the gerbil hippocampus following subacute and chronic restraint stress. Serum corticosterone levels were increased in both the subacute and chronic-stress-groups compared to the control-group: the level in the subacute-stress-group was much higher than that in the chronic-stress-group. We could not find any neuronal damage in all the sub-regions of the hippocampus after both the subacute and chronic restraint stress. In the subacute-stress-group, rps3 immunoreactivity was not different compared to the control-group. However, rps3 immunoreactivity in the chronic-stress-group was decreased compared to the subacute-stress-group: especially, the immunoreactivity was markedly decreased in the pyramidal cells of the hippocampus proper (CA1-CA3 region) and granule cells of the dentate gyrus. In addition, western blot analysis also showed that rpS3 protein levels in the chronic-stress-group were significantly decreased compared to those in the subacute-stress-group. These findings indicate that chronic stress, not subacute stress, can decrease rpS3 immunoreactivity. 相似文献
17.
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19.
Cryptic Expression of the 70-kDa Heat Shock Protein,hsp72, in Gerbil Hippocampus after Transient Ischemia 总被引:2,自引:0,他引:2
The 70 kDa heat shock protein, hsp72, is known to be induced following transient global ischemia in brain, as detected by immunocytochemistry and in situ hybridization techniques. However, while hsp72 mRNA is expressed rapidly following postischemic recirculation, immunocytochemistry fails to detect hsp72 protein for many hours after such insults, even in cell populations that readily express Fos and other proteins encoded by ischemia-induced mRNAs. In the present study, hsp72 expression in gerbil hippocampus was compared by immunocytochemistry and immunoblot methods at several intervals following 10 min ischemia. As established in previous studies, hsp72 immunoreactivity remained undetectable in postischemic neurons at 6 h following such insults. In contrast, immunoblots of dissected gerbil hippocampus demonstrated nearly maximal accumulation of hsp72 at this time point. These results indicate that the protein is present, but cryptic to detection in perfusion-fixed sections, during early recirculation. The constitutively expressed heat shock cognate protein, hsc70, did not show significant changes in level or distribution by either method, except for a decrease in CA1 staining at 48 h. These results confirm that hsp72 rapidly accumulates to high levels in postischemic hippocampus, and suggest that further studies of its subcellular localization during this interval may offer insight into its functional role as a component of the stress response in neurons after such insults. 相似文献
20.
K Nakaso M Kitayama K Kimura T Yanagawa E Ohama K Nakashima T Ishii K Yamada 《Biochemical and biophysical research communications》1999,259(1):91-96
Heme oxygenase-1 (HO-1) is induced under various stresses. Here we report the induction and localization of HO-1 in the rat brain by intraperitoneal administration of kainic acid (KA). Both mRNA and protein of HO-1 were markedly induced by KA treatment, and each maximal induction was observed 24 h after KA administration. In situ hybridization analysis showed that HO-1 mRNA appeared predominantly in glial cells, and confined neurons were positive in the cerebral cortex, basal ganglia, and hippocampal pyramidal cell layer. Immunohistochemical analysis showed that the positive cells in the cerebral cortex and hippocampus were mainly astrocytes and microglia, whereas neurons in the basal ganglia showed intense immunoreactivity. We also demonstrate the dissociation between HO-1 mRNA and protein level in the hippocampal pyramidal neurons, which is known to be vulnerable against excitotoxicity, and discuss the correlation between this dissociation and the vulnerability of hippocampal pyramidal neurons. 相似文献