共查询到20条相似文献,搜索用时 687 毫秒
1.
目的 探讨鼻咽脱落细胞进行DNA倍体和EB病毒编码RNA (EBERs)检测在鼻咽癌诊断中的应用。方法 对 38例经细胞学诊断为鼻咽癌和 8例为正常的鼻咽细胞涂片分别进行图像分析测定细胞DNA含量和EB病毒EBERs原位杂交检测。结果 与病理细胞学诊断相比 ,DNA异倍体分析和EBERs检测诊断癌的敏感性分别为 5 0 %和 92 %,其特异性均为 10 0 %;其阴性预测值分别为 30 %和 72 %。结论 与EBERs检测相比 ,DNA异倍体分析的诊断敏感性和阴性预测值较低 ,差异具有显著性 (分别为P <0 0 0 1和P <0 0 5 )。证明在鼻咽细胞学涂片应用EB病毒原位杂交检测诊断鼻咽癌优于应用DNA异倍体分析诊断。与细胞DNA图像分析相比 ,EB病毒原位杂交检测具有客观、实验条件简单等优点 ,在鼻咽癌可疑病人的早期诊断和鉴别诊断上具有重要的实用价值。 相似文献
2.
3.
4.
西北农林科技大学动物科技学院 (陕西省农业分子生物学重点实验室 )雷初朝、陈宏和河南科技大学雷雪芹等科研人员对 3头南阳黄牛的线粒体DNAD 1 0 0p区 91 0bp的核苷酸序列进行了分析 ,发现此 3头牛D 1 0 0p区的核苷酸序列有 3种单倍体型 ,其 1、2和 3号D 1 0 0p区的平均核苷酸变异率分别为 0 .4 4%、4 .84 %和 0 .4 4%。其高变区的平均核苷酸变异率分别为 0 .81 %、7.5 7%和 0 .0 0 %。 1号牛的核苷酸变异类型有转换一种形式 ,2号牛的核苷酸变异类型有转换、颠换、插入和缺失 4种形式 ,3号牛核苷酸变异类型有转换和插入两种形式。此 3… 相似文献
5.
短额负蝗的营养成分与利用评价 总被引:13,自引:0,他引:13
本文分析了短额负蝗AtractomorphasinensisBolivar的营养成分 ,包括蛋白质、氨基酸、总糖、粗脂肪、脂肪酸及维生素B1,B2 ,C和E。此种昆虫粗蛋白含量为 2 2 4 5 %~ 32 5 9% ,粗脂肪含量为 2 87%~4 91 % ,总糖为 0 0 95 %。在分析其营养价值的基础上 ,进行了开发利用评价。 相似文献
6.
腌制蔬菜营养成分的测定 总被引:2,自引:0,他引:2
分析了腌制蔬菜中的泡菜 ,盐浸渍菜中所含的主要营养成分的种类及其相对含量。结果显示 :泡菜和盐浸渍菜中水分、灰分、粗蛋白、粗脂肪和粗纤维的含量分别为 4 6 1% ,15 95 % ,10 2 8% ,2 19% ,2 3 2 9%和 3 11% ,2 6 49% ,15 19% ,2 42 % ,2 0 0 5 %。其氨基酸总量为 74 5 0 45mg/g ,6 6 4876mg/g ,其中必需氨基酸占 37 46 39%和43 6 76 1%。 相似文献
7.
8.
湿度和光照对桑白盾蚧种群生长的影响 总被引:5,自引:0,他引:5
本文分析了 4组不同湿度和 3组光照条件下桑白盾蚧 Pseudaulacapis pentagona(Targioni-Tozzetti)的种群生长状况 ,组建生命表。相对湿度在 55% ,77% ,80 % ,95%时 ,种群的内禀增长率分别为 :- 0 .0 0 57,0 .0 510 ,0 .0 50 8,0 .0 0 94 ;净增殖率分别为 :0 .6 92 0 ,2 7.2 10 0 ,2 7.6 6 80 ,1.86 0 6 ;后 3者世代增倍时间分别为 13.5910 ,13.6 4 4 6 ,73.7391天 ;光周期在 L∶D=14∶ 10 ,12∶ 12时 ,种群内禀增长率分别为 :0 .0 4 4 5,0 .0 334,净增殖率分别为 2 3.96 2 8,12 .156 2 ,增倍时间为 15.576 3,2 0 .752 0天 ,而 L∶D=10∶ 14时 ,净增殖率为 0 ,内禀增长率和增倍时间都不存在 ,种群将消亡。 相似文献
9.
仿野生鳖卵的磷脂及粗蛋白含量测定 总被引:1,自引:1,他引:0
仿野生鳖卵内容物冻干粉收率为 30 .6 1% ,它的粗蛋白质及磷脂含量分别为 5 0 .0 1%和 12 .74 %。TLC分析结果表明仿野生鳖卵含有 5种磷脂成份 ,而鹌鹑卵及猪脑仅含有 3种磷脂 相似文献
10.
陕西三种特种稻米氨基酸及品质分析 总被引:3,自引:1,他引:2
以陕西洋县产茉莉香米、红香米和绿香粳米等 3种特种稻米为材料 ,分析了氨基酸及其它品质指标。结果表明 ,茉莉香米、红香米和绿香粳米均含有 1 7种氨基酸 ,氨基酸总含量 (mg/ 1 0 0 g)分别为 730 0、72 70、74 90 ;除Trp外 ,7种必需氨基酸占氨基酸总量分别为 34 .6 %、34 .5 %、34 .8% ;Lys含量 (mg/ 1 0 0g)分别为 5 5 0、5 2 0、5 30 ;粗蛋白质含量分别是 9.94 %、9.2 3%、8.5 2 % ;Cu含量 (mg/ 1 0 0g)分别是 0 .6 2、0 .94、0 .79;Zn含量 (mg/ 1 0 0 g)分别是 1 .99、1 .75、2 .0 2 ;Fe含量 (mg/ 1 0 0 g)分别是 1 .5 5、3.70、4 .5 0 ;直链淀粉含量分别是 1 5 .6 %、3.7%、1 5 .1 % ;胶稠度分别是 33mm、4 0 .6mm、78mm ;糊化温度分别是 6 .1级、6 .8级、7级 ;水分含量分别是 1 1 .2 7%、1 1 .5 5 %、1 2 .5 2 %。这 3种特种稻品质较优 ,具有推广价值 相似文献
11.
The objectives of this research were to evaluate the stability of parthenolide in feverfew solution state and powdered feverfew (solid state), and explore the compatibility between commonly used excipients and parthenolide in feverfew. Feverfew extract solution was diluted with different pH buffers to study the solution stability of parthenolide in feverfew. Powdered feverfew extract was stored under 40 degrees C/0% approximately 75% relative humidities (RH) or 31% RH/5~50 degrees C to study the influence of temperature and relative humidity on the stability of parthenolide in feverfew solid state. Binary mixtures of feverfew powered extract and different excipients were stored at 50 degrees C/ 75% RH for excipient compatibility evaluation. The degradation of parthenolide in feverfew solution appears to fit a typical first-order reaction. Parthenolide is comparatively stable when the environmental pH is in the range of 5 to 7, becoming unstable when pH is less than 3 or more than 7. Parthenolide degradation in feverfew in the solid state does not fit any obvious reaction model. Moisture content and temperature both play important roles affecting the degradation rate. After 6 months of storage, parthenolide in feverfew remains constant at 5 degrees C/31% RH. However, approximately 40% parthenolide in feverfew can be degraded if stored at 50 degrees C/31% RH. When the moisture changed from 0% to 75% RH, the degradation of parthenolide in feverfew increased from 18% to 32% after 6-month storage under 40 degrees C. Parthenolide in feverfew exhibits good compatibility with commonly used excipients under stressed conditions in a 3-week screening study. 相似文献
12.
Previous studies have shown that extracts of the aromatic herb feverfew (Tanacetum parthenium) and one of its bioactive components, parthenolide, have anti-inflammatory properties in vivo and in vitro. We examined both crude feverfew extracts and purified parthenolide for their ability to modulate adhesion molecule expression in human synovial fibroblasts. Pretreatment of synovial fibroblasts with either feverfew extracts or purified parthenolide could inhibit the expression of intercellular adhesion molecule-1 (ICAM-1) induced by the cytokines IL-1 (up to 95% suppression), TNF-alpha (up to 93% suppression), and, less strongly, interferon-gamma (up to 39% suppression). Inhibition of ICAM-1 was dose and time dependent; as little as a 30-min pretreatment with feverfew resulted in inhibition of ICAM-1. The decrease in ICAM-1 expression was accompanied by a decrease in T-cell adhesion to the treated fibroblasts. Other herbal extracts with reported anti-inflammatory effects were similarly tested and did not decrease ICAM-1 expression. The modulation of adhesion molecule expression may be an additional mechanism by which feverfew mediates anti-inflammatory effects. 相似文献
13.
The objectives of this research are: (1) to assess selected formulation-relevant physical properties of several commercial
Feverfew extracts, including flowability, hygroscopicity, compressibility and compactibility (2) to develop and validate a
suitable extraction method and HPLC assay, and (3) to determine the parthenolide content of several commercial Feverfew extracts.
Carr’s index, minimum orifice diameter and particle-particle interaction were used to evaluate powder flowability. Hygroscopicity
was evaluated by determining the equilibrium moisture content (EMC) after storage at various % relative humidities. Heckle
analysis and compression pressure-radial tensile strength relationship were used to represent compression and compaction properties
of feverfew extracts. An adapted analytical method was developed based on literature methods and then validated for the determination
of parthenolide in feverfew. The commercial extracts tested exhibited poor to very poor flowability. The comparatively low
mean yield pressure suggested that feverfew extracts deformed mainly plastically. Hygroscopicity and compactibility varied
greatly with source. No commercial feverfew extracts tested contained the label claimed parthenolide. Even different batches
from the same manufacturer showed significantly different parthenolide content. Therefore, extract manufactures should commit
to proper quality control procedures that ensure accurate label claims, and supplement manufacturers should take into account
possible differences in physico-chemical properties when using extracts from multiple suppliers. 相似文献
14.
Parthenolide, the main bioactive compound of the medicinal plant feverfew (Tanacetum parthenium), is a promising anti-cancer drug. However, the biosynthetic pathway of parthenolide has not been elucidated yet. Here we report on the isolation and characterization of all the genes from feverfew that are required for the biosynthesis of parthenolide, using a combination of 454 sequencing of a feverfew glandular trichome cDNA library, co-expression analysis and metabolomics. When parthenolide biosynthesis was reconstituted by transient co-expression of all pathway genes in Nicotiana benthamiana, up to 1.4 μg g−1 parthenolide was produced, mostly present as cysteine and glutathione conjugates. These relatively polar conjugates were highly active against colon cancer cells, with only slightly lower activity than free parthenolide. In addition to these biosynthetic genes, another gene encoding a costunolide and parthenolide 3β-hydroxylase was identified opening up further options to improve the water solubility of parthenolide and therefore its potential as a drug. 相似文献
15.
16.
Brown A; Edwards C; Hartman T; Marshall J; Smith R; Davey M; Power J; Lowe K 《Journal of experimental botany》1999,50(333):435-444
Novel sexual hybrids have been produced between the medicinally-important
species T. parthenium (L.) Schultz-Bip. (feverfew) and
T. vulgare (L.) Schultz-Bip. (tansy). Morphologically,
the F1 hybrids were more closely aligned to feverfew than to tansy,
although notable differences were observed in floral and leaf morphologies
of the hybrid plants compared to both parental species. Ultrastructurally,
the lower epidermal leaf surfaces of the F1 hybrids displayed
characteristics from both parents, with glandular trichome morphology and
density similar to that of feverfew, but non-glandular trichome density
comparable to that of tansy. Diploid F1
(2n2x18) hybrids and their
parental progenitors were analysed biochemically, using chromatographic
techniques. The bioactive germacranolide, parthenolide, was present in high
concentrations [1.720.16% dry leaf weight
(mean s.d., n5)] in
leaf extracts from feverfew, but to a much lesser extent in both tansy and
the F1 hybrids (<0.03% and <0.01% dry leaf weight,
respectively). Whilst secondary metabolite accumulation in the leaves of
the F1 hybrids was largely additive compared to the parental species, novel
compounds were also detected in the F1 hybrids by HPLC, GC and TLC,
indicating the expression of new metabolic pathways as a result of sexual
hybridization. Pharmacologically, leaf extracts from the F1 hybrids
inhibited human polymorphonuclear leucocyte (PMNL) activity in
vitro, despite containing only trace amounts of parthenolide,
the principal bioactive moiety from feverfew. This, in conjunction with the
isolation of a fraction from the crude F1 leaf extract displaying
significant (>5%) inhibition of PMNL activity, provides further
evidence that parthenolide is not the sole determinant of pharmacological
activity in the genus Tanacetum.Keywords:
Feverfew, tansy, sexual hybridization, parthenolide, novel
compounds.
相似文献
17.
S Heptinstall W A Groenewegen P Spangenberg W L?sche 《Folia haematologica (Leipzig, Germany : 1928)》1988,115(4):447-449
Extracts of feverfew inhibit platelet aggregation and the platelet release reaction. The active components are believed to be sesquiterpene lactones such as parthenolide. Evidence is presented that inhibition of platelet behaviour is via neutralization of sulphydryl groups either inside or outside the cell. The precise nature of the sulphydryl groups that are susceptible to feverfew and are involved in platelet aggregation and the release reaction have not yet been defined. 相似文献
18.
Carlisi D D'Anneo A Angileri L Lauricella M Emanuele S Santulli A Vento R Tesoriere G 《Journal of cellular physiology》2011,226(6):1632-1641
This article shows that HepG2, Hep3B, and SK-Hep1 cells, three lines of human hepatocellular carcinoma (HCC) cells, are resistant to apoptosis induced by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Parthenolide, a sesquiterpene lactone found in European feverfew, has been shown to exert both anti-inflammatory and anti-cancer activities. This article demonstrates that co-treatment with parthenolide and TRAIL-induced apoptosis with synergistic interactions in the three lines of HCC cells. In order to explain these effects we ascertained that parthenolide increased either at protein or mRNA level the total content of death receptors TRAIL-R1 and -R2 as well as their surface expression. These effects were found in the three cell lines in the case of TRAIL-R2, while for TRAIL-R1 they were observed in HepG2 and SK-Hep1 cells, but not in Hep3B cells. We suggest that the effects of parthenolide on death receptors depend on the decrease in the level of phosphorylated and active forms of STAT proteins, an event which could be a consequence of the inhibitory effect exerted by parthenolide on the activation of JAK proteins. In agreement with this hypothesis treatment with STAT3 siRNA increased in HCC cells the effect of parthenolide on the expression of death receptors. Sensitization by parthenolide to TRAIL stimulated in the three cell lines the extrinsic mechanism of apoptosis with the activation of both caspases 8 and 3, whereas mitochondria were not involved in the process. Our results suggest that co-treatment with parthenolide and TRAIL could represent a new important therapeutic strategy for hepatic tumors. 相似文献
19.
Toll-like receptors (TLRs) play an important role in induction of innate immune responses for host defense against invading
microbial pathogens. Microbial component engagement of TLRs can trigger the activation of myeloid differential factor 88 (MyD88)-
and toll-interleukin-1 receptor domain-containing adapter inducing interferon-β (TRIF)-dependent downstream signaling pathways.
Parthenolide, an active ingredient of feverfew (Tanacetum parthenium), has been used for centuries to treat many chronic diseases. Parthenolide inhibits the MyD88-dependent pathway by inhibiting
the activity of inhibitor-κB kinase. However, it is not known whether parthenolide inhibits the TRIF-dependent pathway. To
evaluate the therapeutic potential of parthenolide, its effect on signal transduction via the TRIF-dependent pathway of TLRs
induced by lipopolysaccharide (LPS) or polyinosinic-polycytidylic acid (poly [I:C]) was examined. Parthenolide inhibited nuclear
factor-κB and interferon regulatory factor 3 activation induced by LPS or poly[I:C], and the LPS-induced phosphorylation of
interferon regulatory factor 3 as well as interferon-inducible genes such as interferon inducible protein-10. These results
suggest that parthenolide can modulate TRIF-dependent signaling pathways of TLRs, and may be the basis of effective therapeutics
for chronic inflammatory diseases. 相似文献
20.
Hwang DR Wu YS Chang CW Lien TW Chen WC Tan UK Hsu JT Hsieh HP 《Bioorganic & medicinal chemistry》2006,14(1):83-91
Hepatitis C virus (HCV) infection is a severe liver disease that often leads to liver cirrhosis and hepatocellular carcinoma (HCC). Current therapy is inadequate to conquer this viral disease. In this study, we identified parthenolide (1), an active component in feverfew, a popular remedy for fever and migraine, as a lead compound with an EC50 value of 2.21 microM against HCV replication in a subgenomic RNA replicon assay system. Parthenolide is able to potentiate the interferon alpha-exerted anti-HCV effect. Several commercially available sesquiterpene lactones (2-5) structurally analogous to parthenolide and a series of synthesized Michael-type adducts of parthenolide (12-18) also exhibit micromolar concentrations for anti-HCV activities. Structure-activity relationship was elucidated to reveal that the spatial arrangement of the terpenoid skeleton fused with an alpha-methylene-gamma-lactone moiety produces maximal anti-HCV activity. In addition, a strong anti-HCV potency indicates a possibility of secondary amino adducts (12-18) converting back to parthenolide or being replaced by the nucleophilic residues of proteins inside cells. This work shows that screening of natural products is a viable and fast way for identifying novel molecular diversity as potential drug leads. 相似文献