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1.
Hundung Limestone habitat, Manipur, India is an unexplored site for microbial diversity studies. Using polyphasic taxonomy, a Streptomyces strain, MBRL 172 T, has been characterized. The strain was found to show highest 16S rRNA gene sequence similarity with Streptomyces coeruleofuscus NBRC 12757 T (99.2 %). The DNA relatedness between MBRL 172 T and S. coeruleofuscus NBRC 12757 T, and between MBRL 172 T and Streptomyces nogalater NBRC 13445 T, were 36.8 ± 4.4 and 52.5 ± 2.7 %, respectively. Strain MBRL 172 T was found to contain ll-diaminopimelic acid as the diagnostic diamino acid and glucose, mannose and xylose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones detected were MK-9(H 6) and MK-9(H 8). The cellular fatty acids identified were mainly saturated fatty acids: anteiso-C 15:0, iso-C 16:0 and iso-C 15:0. Based on differences in the biochemical and molecular characteristics from its closest relatives, the strain can be proposed to represent a novel taxon in the genus Streptomyces, for which the name Streptomyces canchipurensis is proposed, with the type strain MBRL 172 T (=JCM 17575 T = KCTC 29105 T). 相似文献
2.
A Streptomyces-like actinomycete strain, designated as YIM 78087 T, was isolated from a sediment sample collected from Hehua hot spring in Tengchong, Yunnan province, south-west China. The taxonomic position of strain YIM 78087 T was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 78087 T belongs to the genus Streptomyces and is closely related to Streptomyces fimbriatus DSM 40942 T, Streptomyces marinus DSM 41968 T and Streptomyces qinglanensis DSM 42035 T (97.18, 97.05 and 97.1 % similarity, respectively). Combined with the low values of DNA–DNA hybridization between strain YIM 78087 T and its closest neighbours, these analyses indicated that this new isolate represents a different genomic species in the genus Streptomyces. The predominant menaquinones of strain YIM 78087 T were identified as MK-9 (H 4) and MK-9 (H 6). The major fatty acids were identified as anteiso-C 15:0 (28.4 %), anteiso-C 17:0 (23.0 %) and iso-C 16:0 (15.1 %). The whole-cell hydrolysates found to contain glucose, mannose and ribose. The DNA G+C content was determined to be 73.0 mol%. Based on the comparative analysis of phenotypic and genotypic characteristics, it is proposed that strain YIM 78087 T represents a novel species of the genus Streptomyces, for which the name Streptomyces calidiresistens sp. nov., is proposed. The type strain is YIM 78087 T (=BCRC 16955 T=DSM 42108 T=JCM 19629 T). 相似文献
3.
An alkaliphilic actinobacterium, designated EGI 80050 T, was isolated from a desert soil sample of Xinjiang, north-west China, and characterized by a polyphasic approach. The isolate was observed to produce purple orange-yellow aerial mycelium and dark orange-yellow substrate mycelium on yeast extract-malt extract agar medium. Whole-cell hydrolysates of strain EGI 80050 T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose, rhamnose and mannose as the main sugars. The major fatty acids identified were C 16:0-iso (36.8 %), C 15:0-anteiso (17.3 %), 15:0-iso (13.2 %) and 14:0-iso (10.5 %). The predominant menaquinones detected were MK-9(H 6) and MK-9(H 8), while the characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylmethylethanolamine and three unknown phospholipids. The G+C content of the genomic DNA was determined to be 67.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated the strain EGI 80050 T to the genus Streptomyces. Levels of 16 rRNA gene sequence similarities between strain EGI 80050 T and Streptomyces candidus NRRL ISP-5141 T, Streptomyces cremeus NBRC 12760 T, Streptomyces spiroverticillatus NBRC 12821 T, Streptomyces violaceorectus NBRC 13102 T, Streptomyces cinereoruber subsp. cinereoruber NBRC 12756 T were 96.7, 96.6, 96.6, 96.6 and 96.6 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain EGI 80050 T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces fukangensis sp. nov. (type strain EGI 80050 T = BCRC 16945 T = JCM 19127 T) is proposed. 相似文献
4.
An actinomycete strain, designated strain LUSFXJ T, was isolated from a soil sample obtained near the Xiangtan Manganese Mine, Central-South China and characterised using a polyphasic taxonomic approach. The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. The DNA–DNA relatedness between this strain and two closely related type strains, Streptomyces echinatus CGMCC 4.1642 T and Streptomyces lanatus CGMCC 4.137 T, were 28.7 ± 0.4 and 19.9 ± 2.0%, respectively, values which are far lower than the 70% threshold for the delineation of a novel prokaryotic species. The DNA G+C content of strain LUSFXJ T is 75.0 mol%. Chemotaxonomic analysis revealed that the menaquinones of strain LUSFXJ T are MK-9(H 6), MK-9(H 8), MK-9(H 2) and MK-8(H 8). The polar lipid profile of strain LUSFXJ T was found to contain diphosphatidylglycerol and an unidentified polar lipid. The major cellular fatty acids were identified as iso-C 15:0, anteiso-C 15:0, iso-C 16:0, C 16:0 and Summed feature 3. Strain LUSFXJ T was found to contain meso-diaminopimelic acid as the diagnostic cell wall diamino acid and the whole cell hydrolysates were found to be rich in ribose, mannose and glucose. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, it is concluded that strain LUSFXJ T represents a novel species of the genus Streptomyces, for which the name S. xiangtanensis sp. nov. is proposed. The type strain is LUSFXJ T (=GDMCC 4.133 T = KCTC 39829 T). 相似文献
5.
A novel actinomycete strain, designated TRM 49605 T, was isolated from a desert soil sample from Lop Nur, Xinjiang, north-west China, and characterised using a polyphasic taxonomic approach. The strain exhibited antifungal activity against the following strains: Saccharomyces cerevisiae, Curvularia lunata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Penicillium citrinum, Candida albicans and Candida tropicalis; Antibacterial activity against Bacillus subtilis, Staphylococcus epidermidis and Micrococcus luteus; and no antibacterial activity against Escherichia coli. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain TRM 49605 T to the genus Streptomyces. Strain TRM 49605 T shows high sequence similarities to Streptomyces roseolilacinus NBRC 12815 T (98.62 %), Streptomyces flavovariabilis NRRL B-16367 T (98.45 %) and Streptomyces variegatus NRRL B-16380 T (98.45 %). Whole cell hydrolysates of strain TRM 49605 T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid and galactose, glucose, xylose and mannose as the major whole cell sugars. The major fatty acids in strain TRM 49605 T were identified as iso C 16:0, anteiso C 15:0, C 16:0 and Summed Feature 5 as defined by MIDI. The main menaquinones were identified as MK-9(H 4), MK-9(H 6), MK-9(H 8) and MK-10(H 6). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. The G+C content of the genomic DNA was determined to be 71.2 %. The DNA–DNA relatedness between strain TRM 49605 T and the phylogenetically related strain S. roseolilacinus NBRC 12815 T was 60.12 ± 0.06 %, which is lower than the 70 % threshold value for delineation of genomic prokaryotic species. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain TRM 49605 T (=CCTCC AA2015026 T = KCTC 39666 T) should be designated as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces luozhongensis sp. nov. is proposed. 相似文献
6.
A novel endophytic actinomycete strain, designated KM-1-2 T, was isolated from seeds of Ginkgo biloba at Yangling, China. A polyphasic approach was used to study the taxonomy of strain KM-1-2 T and it was found to show a range of phylogenetic and chemotaxonomic properties consistent with those of members of the genus Streptomyces. The diamino acid of the cell wall peptidoglycan was identified as LL-diaminopimelic acid. No diagnostic sugars were detected in whole cell hydrolysates. The predominant menaquinones were identified as MK-9(H 6) and MK-9(H 8). The diagnostic phospholipids were found to be phosphatidylethanolamine and phosphatidylcholine. The DNA G + C content of the novel strain was determined to be 72.9 mol%. The predominant cellular fatty acids (> 10.0?%) were identified as iso-C 14?:?0, iso-C 16?:?0, C 16?:?0 and C 17?:?0 cyclo. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain is closely related to Streptomyces carpaticus JCM 6915 T (99.3%), Streptomyces harbinensis DSM 42076 T (98.9%) and Streptomyces cheonanensis JCM 14549 T (98.5%). DNA-DNA hybridizations with these three close relatives gave similarity values of 39.1 ± 1.9, 35.8 ± 2.3, and 47.4 ± 2.7%, respectively, which indicated that strain KM-1-2 T represents a novel species of the genus Streptomyces. This is consistent with the morphological, physiological and chemotaxonomic data. Cumulatively, these data suggest that strain KM-1-2 T represents a novel Streptomyces species, for which the name Streptomyces ginkgonis sp. nov. is proposed, with the type strain KM-1-2 T (= CCTCC AA2016004 T = KCTC 39801 T). 相似文献
7.
A novel actinomycete, designated strain NEAU-ycm1 T, was isolated from an edible Chinese black ant ( Polyrhachis vicina Roger) and characterized with a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of streptomycetes. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence show that the novel isolate belongs to the genus Streptomyces and forms a separate subclade. The closest phylogenetic relatives were identified as the type strains of Streptomyces intermedius NBRC 13049 T (97.74 %), Streptomyces aureoverticillatus NRRL B-3326 T (97.69 %), Streptomyces rutgersensis NBRC 12819 T (97.68 %), Streptomyces gougerotii NBRC 3198 T (97.68 %) and Streptomyces diastaticus subsp . diastaticus NBRC 3714 T (97.68 %). Similarities to other type strains of the genus Streptomyces were lower than 97.55 %. A comparison between strain NEAU-ycm1 T and the closest related Streptomyces type strains revealed that it is different from them in morphological, physiological and biochemical characteristics. Therefore, it is proposed that NEAU-ycm1 T (=CGMCC 4.7094 T = DSM 42102 T) represents a novel species of the genus of Streptomyces, for which the name Streptomyces polyrhachii sp. nov. is proposed. 相似文献
8.
A novel Gram-stain positive, spore-forming, aerobic actinomycete, designated strain NEAU-QTH3-11 T, was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, northeast China and characterised using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-QTH3-11 T belongs to the genus Streptomyces and is closely related to Streptomyces rhizosphaerihabitans NBRC 109807 T (99.38%) and Streptomyces mirabilis JCM 4791 T (99.03%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain formed a cluster with S. rhizosphaerihabitans NBRC 109807 T and Streptomyces siamensis NBRC 108799 T (98.62%). The menaquinones were identified as MK-9(H 8), MK-9(H 6) and MK-9(H 4). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C 15:0, iso-C 16:0, C 16:0 and C 15:0. However, multilocus sequence analysis based on five house-keeping genes ( atpD, gyrB, rpoB, recA and trpB), low DNA-DNA hybridization results and some phenotypic, physiological and biochemical properties could differentiate the strain from its close relatives in the genus Streptomyces. Therefore, strain NEAU-QTH3-11 T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces lutosisoli sp. nov. is proposed, with NEAU-QTH3-11 T (=DSM 42165 T=CGMCC 4.7198 T) as the type strain. 相似文献
9.
A novel actinomycete, designated strain KLBMP 4601 T, was isolated from the root of the medicinal plant Curcuma phaeocaulis collected from Sichuan Province, south-west China. The strain produced extensively branched substrate and aerial hyphae that carried straight to flexuous spore chains. Chemotaxonomic properties of this strain were consistent with those of members of the genus Streptomyces. The cell wall of strain KLBMP 4601 T contained ll-diaminopimelic acid as the characteristic diamino acid. The major menaquinone was MK-9(H 4), with minor amounts of MK-9(H 6), MK-9(H 8) and MK-10(H 2). The major fatty acids were C 16:0, iso-C 16:0, C 18:1ω9 c and C 16:1, iso G. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KLBMP 4601 T belongs to the genus Streptomyces and is most closely related to Streptomyces armeniacus JCM 3070 T (97.9 %), Streptomyces pharmamarensis PM267 T (97.6 %) and Streptomyces artemisiae YIM 63135 T (97.5 %). The 16S rRNA gene sequence similarity between strain KLBMP 4601 T and other members of this genus were lower than 97.5 %. DNA–DNA hybridization studies of strain KLBMP 4601 T with the three closest species showed relatedness values of 36.3 ± 4.2 %, 27.3 ± 0.6 %, and 30.9 ± 2.5 %, respectively. On the basis of chemotaxonomic, phenotypic and genotypic characteristics, it is evident that strain KLBMP 4601 T represents a novel species of the genus Streptomyces, for which the name Streptomyces phytohabitans sp. nov. is proposed. The type strain is KLBMP 4601 T (=KCTC 19892 T = NBRC 108772 T). 相似文献
10.
A novel actinobacterial strain, designated TRM SA0054 T, was isolated from the roots of Sophora alopecuroides grown in Alar, Xinjiang, north-west China, and characterised by a polyphasic taxonomic approach. Phylogenetic analysis showed that strain TRM SA0054 T has 16S rRNA gene sequence similarity of 98.22% with Streptomyces barkulensis RC 1831 T. Whole cell hydrolysates of strain TRM SA0054 T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid and ribose, glucose and xylose as the major whole cell sugars. The major fatty acids were identified as iso-C 16:0, iso-C 16:1 G, anteiso-C 17:0, anteiso-C 15:0, C 16:0, anteiso-C 17:1 ω9c. The main menaquinones were determined to be MK-8 (H 4), MK-9 (H 6) and MK-9 (H 8). The polar lipids were identified as diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified lipid. The G?+?C content of the genomic DNA was determined to be 73.04%. Strain TRM SA0054 T has a relatively low DNA–DNA relatedness value with Streptomyces barkulensis RC 1831 T as determined by calculating the average nucleotide identity value (ANI?=?84.1%). Based on the phenotypic, chemotaxonomic and phylogenetic data, it is concluded that strain TRM SA0054 T should be designated as a novel species of the genus Streptomyces, for which the name Streptomyces carminius sp. nov. is proposed, with type strain TRM SA0054 T (=?CCTCC AA 2016041 T?=?KCTC39903 T). 相似文献
11.
An arsenite-oxidizing bacterium, strain S2-3H T, was isolated from arsenic-contaminated soil sample collected from Dantchaeng district, Suphanburi province, Thailand and was characterized based on polyphasic taxonomic study. The strain was observed to be a Gram-stain negative, aerobic, yellow pigmented, non-spore forming and rod-shaped bacterium. Major menaquinone was MK-6. Iso-C 15:0, iso-C 15:0 3OH, C 16:1 ω7c/C 16:1 ω6c, C 16:0, iso-C 17:0 3OH, and C 16:0 3OH were the predominant cellular fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, unidentified phospholipids and unidentified aminophospholipids. The DNA G+C content was 37.0 mol%. Phylogenetic analysis using 16S rRNA sequence showed that strain S2-3H T is affiliated to the genus Flavobacterium, and is closely related to F. defluvii KCTC 12612 T (97.0 %) and F. johnsoniae NBRC 14942 T (97.0 %). The strain S2-3H T could be clearly distinguished from the related Flavobacterium species by its physiological and biochemical characteristics as well as its phylogenetic position and DNA–DNA relatedness. Therefore, the strain represents a novel species of the genus Flavobacterium, for which the name Flavobacterium arsenitoxidans sp. nov. (type strain S2-3H T = KCTC 22507 T = NBRC 109607 T = PCU 331 T = TISTR 2238 T) is proposed. 相似文献
12.
During an investigation exploring potential sources of novel thermophilic species and natural products, a novel thermophilic and alkaliphilic actinomycete with alkaline cellulase producing ability, designated strain 4-2-13 T, was isolated from soil of a tropical rainforest in Xishuangbanna, Yunnan province, China. The morphological and chemotaxonomic characteristics of strain 4-2-13 T are consistent with those of the members of the genus Streptomyces. The strain forms extensively branched aerial mycelia and substrate mycelia. Spiral spore chains were observed on aerial mycelia; spores were oval to cylindrical, with smooth surfaces. The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan. The whole cell hydrolysates were found to contain glucose and ribose. The cellular fatty acid profile mainly consists of anteiso-C 17:0 and iso-C 16:0. The menaquinones were identified as MK-9(H 8), MK-10(H 6) and MK-9(H 6). The polar lipids profile were found to consist of diphosphatidylglycerol, phosphatidylmethylethanolamine, a ninhydrin-positive glycophospholipid, phosphatidylinositol, phosphatidylglycerol and unidentified glycolipids. The 16S rRNA gene sequence analysis showed that the organism belongs to the genus Streptomyces and in the 16S rRNA gene tree it formed a distinct phyletic line together with the closely related type strain Streptomyces burgazadensis Z1R7 T (95.2% sequence similarity). However, the phenotypic characteristics of strain 4-2-13 T are significantly different from those of S. burgazadensis Z1R7 T. Based on the phenotypic, chemotaxonomic and phylogenetic characteristics, strain 4-2-13 T represents a novel species in the genus Streptomyces, for which the name Streptomyces thermoalkaliphilus sp. nov. is proposed. The type strain is 4-2-13 T (= DSM 42159 T = CGMCC 4. 7205 T). 相似文献
13.
An actinomycete capable of lysing cyanobacteria, strain JXJ 0074 T, was isolated from a soil sample collected from Jiangxi province, south China, and characterized by using polyphasic taxonomy. The new isolate showed morphological and chemotaxonomic properties typical of members of the genus Streptomyces. Phylogenetic analysis of the near-complete 16S rRNA gene sequence indicated that strain JXJ 0074 T should be affiliated to the genus Streptomyces and exhibited highest similarities to Streptomyces shenzhenensis DSM 42034 T (98.99 %) and Streptomyces lucensis NBRC 13056 T (98.60 %), while the similarities to other members of the genus are lower than 98.22 % similarity. However, the DNA–DNA hybridization values between strain JXJ 0074 T and S. shenzhenensis DSM 42034 T or S. lucensis NBRC 13056 T were 46.2 ± 2.6 and 32.6 ± 3.1 %, respectively. Thus, on the basis of the polyphasic data, strain JXJ 0074 T represents a novel species of the genus Streptomyces, for which the name Streptomyces jiujiangensis sp. nov. is proposed. The type strain is JXJ 0074 T (= BCRC 16953 T = KCTC 29262 T). 相似文献
14.
A total of 37 actinobacteria were isolated from eighteen lichen samples collected in Thailand. Based on the 16S rRNA gene sequences, they were identified into five genera including Actinoplanes (1 strain), Actinomadura (1 strain), Pseudosporangium (1 strain), Wangella (1 strain) and Streptomyces (33 strains). Among these isolates, strain Ptm05T, Ptm01 and Ptm12 showed low 16S rRNA gene similarity and was selected for the further taxonomic study using the polyphasic approach. These strains showed the highest 16S rRNA gene sequence similarity with Streptomyces sparsogenes ATCC 25498T (97.44–97.72%). Strain Ptm05T was selected for the type strain. The chemical cell composition of the strain was similar to the members of Streptomyces genus. LL-diaminopimelic acids were detected in the peptidoglycan. Menaquinones were MK-9(H8) and MK-9(H6). Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified phospholipid, one unidentified glycolipid and one unidentified lipid were detected as the polar lipids. The predominant cellular fatty acids are anteiso-C15:0, iso-C15:0, iso-C16:0, iso-C17:0 and C16:0. The dDNA-DNA hybridization values among strain Ptm05T and its closely related Streptomyces type strains were 17.2–18.0%. In addition, the ANIb and ANIm between strain Ptm05T and related Streptomyces type strains were ranged from 75.69 to 76.13% and 85.21 to 85.35%, respectively. Based on phenotypic and genomic evidence, strain Ptm05T (=?TBRC 14546T?=?NBRC 115203T) represents the novel species of the genus Streptomyces for which the name Streptomyces parmotrematis sp. nov. is proposed. This study showed that the lichens are the promising source of the novel actinobacterial taxa. 相似文献
15.
A Gram-positive, aerobic, non-motile actinobacterial strain, designated YIM C01235 T, was isolated from a soil sample collected from the Swallow Cave, Yunnan province, south-west China. The isolate grew at 10–30 °C, pH 6.0–9.0 and 0–8 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed highest similarity to Saccharopolyspora gloriosae YIM 60513 T (96.8 %), and lower 16S rRNA gene sequence similarities (95.1–96.7 %) with the other species of the genus Saccharopolyspora. The whole-cell hydrolysates contained meso-diaminopimelic acid ( meso-DAP), arabinose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unknown glycolipids, two unknown phospholipids and one polar lipid. MK-9(H 4) was the predominant menaquinone. The major fatty acids were iso-C 15:0, iso-C 16:0, anteiso-C 15:0 and anteiso-C 17:0. Mycolic acids were not detected. The genomic DNA G+C content was 69.1 mol%. These chemotaxonomic data, together with its morphological properties, were consistent with the assignment of strain YIM C01235 T to the genus Saccharopolyspora. The results of physiological and biochemical tests allowed strain YIM C01235 T to be differentiated phenotypically from all the recognized Saccharopolyspora species. On the basis of evidence from this polyphasic study, the novel species Saccharopolyspora cavernae sp. nov. is proposed. The type strain is YIM C01235 T (=DSM 45825 T = CCTCC AA 2012022 T). 相似文献
16.
A Gram-positive bacterium, designated SCSIO 15042 T, was isolated from a sediment of the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew at 20–60 °C, pH 6.0–10.0 and it could grow with up to 10 % (w/v) NaCl. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and an unknown polar lipid. The only menaquinone was determined to be MK-7. The major fatty acids were identified as C 16:1 ω7 c/C 16:1 ω6 c, C 16:0, iso-C 15:0, anteiso-C 15:0, and iso-C 16:0. The DNA G+C content of strain SCSIO 15042 T was determined to be 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SCSIO 15042 T to the genus Bacillus. Levels of 16S rRNA gene sequence similarities between strain SCSIO 15042 T and Bacillus herbersteinensis D-1-5a T, Bacillus infantis SMC 4352-1 T, Bacillus novalis LMG 21837 T and Bacillus drentensis LMG 21831 T were 96.2, 96.2, 96.1 and 96.1 %, respectively. Based on the evidence of the present polyphasic study, strain SCSIO 15042 T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus abyssalis sp. nov. is proposed. The type strain is SCSIO 15042 T (=DSM 25875 T = CCTCC AB 2012074 T = NBRC 109102 T). 相似文献
17.
A novel halophilic actinomycete strain, H23 T, was isolated from a Saharan soil sample collected in Djamâa (Oued Righ region), El-Oued province, South Algeria. Strain H23 T was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23 T had 16S rRNA gene sequence similarities ranging from 97.8 % ( Actinopolyspora xinjiangensis TRM 40136 T) to 94.8 % ( Actinopolyspora mortivallis DSM 44261 T). The strain grew optimally at pH 6.0–7.0, 28–32 °C and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerial mycelium produced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23 T had MK-10 (H 4) and MK-9 (H 4) as the predominant menaquinones. The whole micro-organism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C 17:0 (37.4 %), iso-C 17:0 (14.8 %), iso-C 15:0 (14.2 %), and iso-C 16:0 (13.9 %). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23 T (=DSM 45501 T = CCUG 63368 T = MTCC 11562 T). 相似文献
18.
A halotolerant actinomycete strain, designated strain KLBMP 1305 T, was isolated from a salt marsh plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1305 T is a member of the genus Saccharopolyspora. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1305 T was most closely related to ‘ Saccharopolyspora pathumthaniensis’ S582 T (99.31 %), ‘ Saccharopolyspora endophytica’ YIM 61095 T (99.17 %) and Saccharopolyspora tripterygii YIM 65359 T (99.15 %); similarity to other type strains of the genus Saccharopolyspora was <97.2 %. The organism had chemical and morphological features consistent with its classification in the genus Saccharopolyspora such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was MK-9(H 4). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unknown glycolipid and an unknown lipid. The major fatty acids were iso-C 16:0, iso-C 15:0, anteiso-C 15:0, anteiso-C 17:0 and sum in feature 8 (18:1ω7 c/18:1ω6 c) . The G+C content of the genomic DNA of the type strain was 68.7 mol%. DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate represents a novel species, for which the name Saccharopolyspora dendranthemae sp. nov. is proposed. The type strain is KLBMP 1305 T (=KCTC 19889 T = NBRC 108675 T). 相似文献
19.
A Gram-positive, aerobic, non-motile and pale yellow colour actinobacterial strain, designated SYP-B575 T, was isolated from rhizosphere soil of Panax notoginseng. The optimal growth of the strain was found to occur at 28 °C, pH 7.0 and without NaCl. Phylogenetic analysis indicated that strain SYP-B575 T clearly belongs to the genus Sinomonas and should be considered as a candidate of novel species within this genus. The 16S rRNA gene sequence similarities between strain SYP-B575 T and the other Sinomonas type strains ranged from 97.3 to 96.0 %. The predominant isoprenoid quinone was identified as MK-9(H 2) and the major fatty acids were identified as anteiso-C 15:0 and anteiso-C 17:0. The polar lipids were found to consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and glycolipids. The major cell-wall amino acids were identified as Lys, Ala, Glu, Gly and Ser. The whole-cell sugars were identified as mannose, ribose, rhamnose, glucose and galactose. The G+C content of the genomic DNA was determined to be 66.6 mol%. The DNA–DNA relatedness values between SYP-B575 T and its closest phylogenetic neighbours were lower than 35.5 %. On the basis of this polyphasic taxonomic study, strain SYP-B575 T represents a novel species of the genus Sinomonas, for which the name Sinomonas notoginsengisoli sp. nov. is proposed. The type strain is SYP-B575 T (=DSM 27685 T = KCTC 29237 T). 相似文献
20.
A novel halophilic, filamentous actinomycete, designated TRM 4064 T, was isolated from a hypersaline habitat in Sichuan Province, China. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of strain TRM 4064 T showed that it was most closely related to Actinopolyspora mortivallis (99.1 % sequence similarity). The sequence similarities between strain TRM 4064 T and other Actinopolyspora species with validly-published names were <97.0 %. However, it had relatively low mean values for DNA–DNA relatedness with the A. mortivallis DSM 44261 T (23.2 %). Optimal growth occurred at 37 °C, pH 7.0 and in the presence of 13 % (w/v) NaCl. The whole-cell sugar pattern consists of xylose, glucose, ribose and arabinose. The predominant menaquinones are MK-10(H 4) (38.2 %), MK-9(H 4) (25.1 %), MK-9(H 2) (28.6 %) and MK-8(H 4) (7.3 %). The major fatty acids are anteiso-C 17:0 (36.9 %) and iso-C 17:0 (19.3 %). The diagnostic phospholipids detected were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylinositol (PI) and two unknown phospholipids. The G+C content of the genomic DNA of the type strain is 66.3 mol%. Strain TRM 4064 T therefore represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora dayingensis sp. nov. is proposed. The type strain is TRM 4064 T (= KCTC 19979 T = CCTCC AA 2010010 T). 相似文献
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