| 九种常用杀虫剂对二化螟线粒体ATPase活力的抑制作用 |
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| 引用本文： | 李海平,冯涛,陶岭梅,刘学,姜辉,林荣华,梁沛,高希武.九种常用杀虫剂对二化螟线粒体ATPase活力的抑制作用[J].昆虫学报,2006,49(2):254-259. |
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| 作者姓名： | 李海平 冯涛 陶岭梅 刘学 姜辉 林荣华 梁沛 高希武 |
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| 作者单位： | 中国农业大学昆虫学系,北京100094 |
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| 基金项目： | 科技部科研项目;中国科学院资助项目 |
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| 摘 要： | 研究了二化螟Chilo suppressalis线粒体Na+-K+-ATPase和Ca2＋-Mg2＋-ATPase的生物化学性质以及9种常用杀虫剂对这两种酶活性的影响。结果表明, 二化螟线粒体Na+-K+-ATPase和Ca2＋-Mg2＋-ATPase的最适反应条件为pH值7.4，温度37℃。 Na+-K+-ATPase的米氏常数（Km）为0.42 mmol/L，最大反应速度（Vmax）为302.47 nmol/(min·mg) 。Ca2＋-Mg2＋-ATPase的Km为0.40 mmol/L，Vmax为128.04 nmol/(min·mg)。药剂浓度为1×10-4 mol/L时，5种菊酯类杀虫剂对离体ATPase活性抑制的顺序为：溴氰菊酯>联苯菊酯>百树菊酯>三氟氯氰菊酯和氟硅菊酯；对二化螟Na+-K+-ATPase的抑制率分别为40.12％、39.69％、27.27％、19.49％和18.71％；对Ca2＋-Mg2＋-ATPase的抑制率分别为29.27％、23.78％、19.88％、11.64%和14.34％。硫丹对二化螟Na+-K+-ATPase和Ca2＋-Mg2＋-ATPase的抑制率均为17.46％。甲胺磷和呋喃丹对Ca2＋-Mg2＋-ATPase的抑制率分别为27.16％和17.42％，对Na+-K+-ATPase则几乎没有抑制作用。实验结果还表明, 在1.6×10-7～1×10-4 mol/L的浓度范围内，上述9种杀虫剂对二化螟ATPase活性的抑制率存在明显的剂量-效应关系。
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| 关 键 词： | 二化螟 腺苷三磷酸酶 杀虫剂 酶活性 抑制率 |
| 文章编号： | 0454-6296(2006)02-0254-06 |
| 收稿时间： | 07 5 2005 12:00AM |
| 修稿时间： | 09 23 2006 12:00AM |
Inhibition of ATPase activity in mitochondria of Chilo suppressalis by nine common insecticides |
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| LI Hai-Ping,FENG Tao,TAO Ling-Mei,LIU Xue,JIANG Hui,LIN Rong-Hua,LIANG Pei,GAO Xi-Wu.Inhibition of ATPase activity in mitochondria of Chilo suppressalis by nine common insecticides[J].Acta Entomologica Sinica,2006,49(2):254-259. |
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| Authors: | LI Hai-Ping FENG Tao TAO Ling-Mei LIU Xue JIANG Hui LIN Rong-Hua LIANG Pei GAO Xi-Wu |
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| Abstract: | The characteristics of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in mitochondria of Chilo suppressalis and inhibition of these two types of ATPases by nine common insecticides, i.e., deltamethrin, bifenthrin, cyfluthrin, cyhalothrin, salifluofen, endosufan, methamidophos, carbofuran and triazophos, were determined. The results indicated that the optimum pH value of Na+-K+-ATPase was 7.4, and the optimum temperature 37℃ for both Na+-K+-ATPase and Ca2+-Mg2+-ATPase . The Michaelis constant (Km) of Na+-K+-ATPase hydrolyzing ATP was 0.42 mmol/L, not significantly different from that of Ca2+-Mg2+-ATPase (0.40 mmol/L). The maximum velocities (Vmax) hydrolyzing ATP were 302.47 nmol/(mg·min) for Na+-K+-ATPase and 128.04 nmol/(mg·min) for Ca2+-Mg2+-ATPase , respectively. When the final concentration of insecticides was 1×10-4 mol/L, the order of inhibition of to Na+-K+-ATPase by five pyrethroids insecticides was deltamethrin> bifenthrin> cyfluthrin> cyhalothrin and salifluofen, The percentage of inhibition of Na+-K+-ATPase activity was 40.12%, 39.69%, 27.27%, 19.49%, and 18.71%, and the percentage of inhibition of Ca2+-Mg2+-ATPase was 29.27%, 23.78%, 19.88%, 11.64% and 14.34% by the above five pyrethroids respectively when the concentration of inhibitors was 1×10-4 mol/L. The potential of endosufan inhibiting the Na+-K+-ATPase activity was same as its potential inhibiting Ca2+-Mg2+-ATPase , and the percentage of inhibition was 17.46%. Methamidophos and carbofuran possessed the potential of inhibition to Ca2+-Mg2+-ATPase , but no inhibition to Na+-K+-ATPase when their final concentration was 1×10-4 mol/L. The inhibition of ATPase activity by nine insecticides was increased with the increasing of insecticide concentration from 1.6×10-7 to 1×10-4 mol/L. |
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| Keywords: | Chilo suppressalis ATPase insecticides enzyme activity inhibition |
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