| 氨磷汀对氧糖剥夺引起的PC12 细胞缺血再灌注损伤的保护作用研究 |
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| 引用本文： | 张蕾 刘爱秀 王强 李新 贾济 王世全 王百忍 熊利泽. 氨磷汀对氧糖剥夺引起的PC12 细胞缺血再灌注损伤的保护作用研究[J]. 现代生物医学进展, 2011, 11(6): 1110-1113 |
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| 作者姓名： | 张蕾 刘爱秀 王强 李新 贾济 王世全 王百忍 熊利泽 |
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| 作者单位： | 1. 第四军医大学西京医院麻醉科,陕西,西安,710032
2. 第四军医大学西京医院骨科研究所,陕西,西安,710032 |
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| 基金项目： | 国家自然科学基金，国家重点基金 |
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| 摘 要： | 目的:研究氨磷汀对体外培养的神经元样细胞的缺血再灌注损伤的保护作用,为其最终用于临床脑缺血的治疗打下基础。方法:体外培养的PC12细胞氧糖剥夺4h后复氧复糖,给予不同浓度的氨磷汀处理,20h后镜下观察细胞形态学变化,用MTT和LDH检测细胞活力和损伤情况,免疫荧光染色观察凋亡细胞,流式细胞仪计数凋亡细胞的比例。结果:高浓度氨磷汀对正常PC12细胞活力有抑制作用(P<0.05),而低浓度则无。氨磷汀可以提高缺血再灌注损伤PC12细胞活力(P<0.05),减少LDH释放(P<0.05),保护细胞正常形态,抑制细胞凋亡(P<0.05)。结论:氨磷汀对氧糖剥夺引起的神经元样细胞的缺血再灌注损伤具有保护作用。
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| 关 键 词： | 氨磷汀 脑缺血再灌注损伤 PC12细胞 氧糖剥夺 凋亡 |
Protection of Amifostine on Oxygen-glucose Deprivation-induced IschemiaReperfusion Injury in PC12 Cells |
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| ZHANG Lei,LIU Ai-xiu,WANG Qiang,LI Xin,JIA Ji,WANG Shi-quan,WANG Bai-ren,XIONG Li-ze. Protection of Amifostine on Oxygen-glucose Deprivation-induced IschemiaReperfusion Injury in PC12 Cells[J]. Progress in Modern Biomedicine, 2011, 11(6): 1110-1113 |
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| Authors: | ZHANG Lei LIU Ai-xiu WANG Qiang LI Xin JIA Ji WANG Shi-quan WANG Bai-ren XIONG Li-ze |
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| Affiliation: | ZHANG Lei1,LIU Ai-xiu2,WANG Qiang1,LI Xin1,JIA Ji1,WANG Shi-quan1,WANG Bai-ren1,XIONG Li-ze1(1 Department of Anesthesiology,Xijing hospital,The Fourth Military Medical University,Xian 710032,China,2 Department of Anesthesiology,Xi'an NO.1 Hospital,Xian 710002,China) |
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| Abstract: | Objective:To investigate the effect of amifostine on the ischemia-reperfusion injury in neuron-like PC12 cells.Methods:PC12 cells were exposed to oxygen-glucose deprivation(OGD) for 4h followed by 20h reoxygenation.Various doses of amfostine were added into the medium.Cell morphological changes were observed under microscopy and cell viability and integrity were detected by MTT and LDH techniques.Cell apoptosis was detected by Tunel staining and flow cytometry.Results:High concentration of amfostine could i... |
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| Keywords: | Cerebral ischemia reperfusion injury PC12 cell OGD Amifostine Apoptosis |
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