全文获取类型
收费全文 | 1206篇 |
免费 | 95篇 |
专业分类
1301篇 |
出版年
2022年 | 14篇 |
2021年 | 16篇 |
2019年 | 13篇 |
2018年 | 13篇 |
2017年 | 13篇 |
2016年 | 27篇 |
2015年 | 28篇 |
2014年 | 29篇 |
2013年 | 55篇 |
2012年 | 67篇 |
2011年 | 68篇 |
2010年 | 41篇 |
2009年 | 31篇 |
2008年 | 48篇 |
2007年 | 43篇 |
2006年 | 41篇 |
2005年 | 36篇 |
2004年 | 37篇 |
2003年 | 39篇 |
2002年 | 35篇 |
2001年 | 39篇 |
2000年 | 28篇 |
1999年 | 22篇 |
1998年 | 16篇 |
1997年 | 15篇 |
1996年 | 12篇 |
1995年 | 12篇 |
1994年 | 16篇 |
1993年 | 8篇 |
1992年 | 13篇 |
1991年 | 24篇 |
1990年 | 15篇 |
1989年 | 19篇 |
1988年 | 17篇 |
1987年 | 18篇 |
1986年 | 26篇 |
1985年 | 29篇 |
1984年 | 16篇 |
1983年 | 17篇 |
1982年 | 12篇 |
1981年 | 15篇 |
1980年 | 20篇 |
1979年 | 24篇 |
1978年 | 24篇 |
1977年 | 19篇 |
1976年 | 18篇 |
1974年 | 15篇 |
1973年 | 19篇 |
1972年 | 8篇 |
1967年 | 7篇 |
排序方式: 共有1301条查询结果,搜索用时 9 毫秒
31.
M Jadot L Lin D E Sleat I Sohar M S Hsu J Pintar F Dubois S Wattiaux-De Coninck R Wattiaux P Lobel 《The Journal of biological chemistry》1999,274(30):21104-21113
The intracellular transport of soluble lysosomal enzymes relies on the post-translational modification of N-linked oligosaccharides to generate mannose 6-phosphate (Man 6-P) residues. In most cell types the Man 6-P signal is rapidly removed after targeting of the precursor proteins from the Golgi to lysosomes via interactions with Man 6-phosphate receptors. However, in brain, the steady state proportion of lysosomal enzymes containing Man 6-P is considerably higher than in other tissues. As a first step toward understanding the mechanism and biological significance of this observation, we analyzed the subcellular localization of the rat brain Man 6-P glycoproteins by combining biochemical and morphological approaches. The brain Man 6-P glycoproteins are predominantly localized in neuronal lysosomes with no evidence for a steady state localization in nonlysosomal or prelysosomal compartments. This contrasts with the clear endosome-like localization of the low steady state proportion of mannose-6-phosphorylated lysosomal enzymes in liver. It therefore seems likely that the observed high percentage of phosphorylated species in brain is a consequence of the accumulation of lysosomal enzymes in a neuronal lysosome that does not fully dephosphorylate the Man 6-P moieties. 相似文献
32.
Chitinolytic microorganisms isolated from forest soil and from healthy gypsy moth larvae (Porthetria dispar (L.) were screened for their ability to lyse Trichophyton rubrum mycelia. A few of these isolates were mycolytic on both autoclaved and on actively growing, intact, T. rubrum mycelia. Supernatants from these isolates, utilizing live T. rubrum as the sole carbon source, showed the same mycolytic ability. Assays of the supernatants for enzymatic activity revealed exocellular, stable enzymes that releases reducing substances including N-acetylglucosamine from the mycelia. 相似文献
33.
Aline Planchon Gaëlle Durambur Jean‐Baptiste Besnier Carole Plasson Bruno Gügi Sophie Bernard Annabelle Mrieau Jean‐Paul Trouv Caroline Dubois Karine Laval Azeddine Driouich Jean‐Claude Mollet Richard Gattin 《Plant, cell & environment》2021,44(1):304-322
In Normandy, flax is a plant of important economic interest because of its fibres. Fusarium oxysporum, a telluric fungus, is responsible for the major losses in crop yield and fibre quality. Several methods are currently used to limit the use of phytochemicals on crops. One of them is the use of plant growth promoting rhizobacteria (PGPR) occurring naturally in the rhizosphere. PGPR are known to act as local antagonists to soil‐borne pathogens and to enhance plant resistance by eliciting the induced systemic resistance (ISR). In this study, we first investigated the cell wall modifications occurring in roots and stems after inoculation with the fungus in two flax varieties. First, we showed that both varieties displayed different cell wall organization and that rapid modifications occurred in roots and stems after inoculation. Then, we demonstrated the efficiency of a Bacillus subtilis strain to limit Fusarium wilt on both varieties with a better efficiency for one of them. Finally, thermo‐gravimetry was used to highlight that B. subtilis induced modifications of the stem properties, supporting a reinforcement of the cell walls. Our findings suggest that the efficiency and the mode of action of the PGPR B. subtilis is likely to be flax variety dependent. 相似文献
34.
35.
36.
Julien Lagarde Romain Valabrègue Jean-Christophe Corvol Fanny Pineau Isabelle Le Ber Marie Vidailhet Bruno Dubois Richard Levy 《PloS one》2013,8(11)
Progressive supranuclear palsy (PSP) and frontotemporal lobar degeneration (FTD) are two clinicohistological entities that share a severe prefrontal syndrome. To what extent do the cognitive syndrome and the location of the underlying brain atrophy unify or segregate these entities? Here, we examined the clinical and radiological patterns of frontal involvement and the neural bases of the cognitive dysfunctions observed in the Richardson form of PSP and the behavioral variant of FTD (bvFTD). The cognitive profile and grey and white matter volume of PSP (n = 19) and bvFTD (n = 16) patients and control participants (n = 18) were compared using a standard battery of neuropsychological tests and voxel-based morphometry (VBM), respectively. Analyses of correlations between neuropsychological and morphometric data were additionally performed. The severity and qualitative pattern of cognitive dysfunction was globally similar between the two patient groups. Grey matter volume was decreased in widespread frontal areas and in the temporal uncus in bvFTD, while it was decreased in the frontal and temporal lobes as well as in the thalamus in PSP. We also found an unexpected involvement of the frontal rectal gyrus in PSP patients compared to controls. Correlation analyses yielded different results in the two groups, with no area showing significant correlations in PSP patients, while several frontal and some temporal areas did so in bvFTD patients. In spite of minor neuropsychological and morphological differences, this study shows that the patterns of cognitive dysfunction and atrophy are very similar in PSP and bvFTD. However, executive dysfunction in these diseases may stem from partially divergent cortical and subcortical neural circuits. 相似文献
37.
C. Jullien T. Benezech C. Le Gentil L. Boulange-Petermann P. E. Dubois J. P. Tissier 《Biofouling》2013,29(3):163-172
The effect of repeated conditioning procedures (25 runs), consisting of soiling (milk and meat products) and cleaning steps, on the hygienic status, physico-chemical properties and surface chemical composition of stainless steel (SS) surfaces, was investigated. Five SSs differing in grade and finish were used. Both soiling and surface cleaning/conditioning procedures resulted in a similar increase in the surface contamination with carbon, while the changes in the basic component of the surface free energy depended on the conditioning procedure. The passive film was also affected, the Fe/Cr ratio in particular. The hygienic status was also changed, especially with milk as shown by monitoring the number of residual adhering Bacillus cereus spores after contaminating the surface with spores followed by cleaning. The results show that in food environments, the presence and the nature of conditioning molecules play a major role in the hygienic status of SS surfaces. 相似文献
38.
39.