共查询到15条相似文献,搜索用时 62 毫秒
1.
2.
吲哚丁酸处理桉树插条后过氧化物酶活性和同工酶变化与插条生根的关系 总被引:1,自引:0,他引:1
尾叶桉MLA无性系(简称MLA)为难生根植物,属叶桉U6无性系(简称6)和刚果12号桉W5无性系(简称W5)为相对易生根植物。MLA的插条中的过氧化物酶(POD)活性较U6、W5的高。用吲哚丁酸(IBA)处理桉树的插条后,在扦插生根的不同阶段,插条内的POD活性呈现规律性的变化。蛋白质含量呈上升趋势。POD同工酶谱带也随生根的进程出现增多的现象。本文讨论了过氧化物酶与桉树插条生根的关系。 相似文献
3.
4.
在"探索生长素类似物促进插条生根的最适浓度"的探究实验教学中,禀承有效性、直观性、真实性、可操作性和有利于课堂教学目标顺利达成的原则,用大蒜作为材料对此实验进行了有效改进,并对探究过程进行了初步研究,取得了较好效果。 相似文献
5.
IAA—氧化酶活性及同工酶分析方法的改进 总被引:3,自引:0,他引:3
1939年,Mitchell等首先建立了IAA-氧化酶活性的比色方法;1947年Tang(汤玉玮)等在此基础上作了改进。现在通常应用的是Gordon等于1951年在前人基础上再次改进的方法,但此法仍然存在某些不足 相似文献
6.
黄化处理促进绿豆下胚轴插条生根的研究(简报) 总被引:4,自引:1,他引:4
黄化处理后绿豆下胚轴插条的生根数、生根范围、根的鲜重及干重、以及插条基部的可溶性糖含量和多酚氧化酶活性都增加,而酚类物质含量和过氧化物酶活性则减少。 相似文献
7.
IBA对野生蔬菜少花龙葵插条生根的影响 总被引:1,自引:0,他引:1
在室内水培条件下, 研究了吲哚丁酸(IBA)、插条类型及插条留叶方式对野生蔬菜少花龙葵(Solanum photeinocarpum)插条生根的影响。结果表明, 5~35 mg L-1 IBA处理的少花龙葵半叶嫩枝插条生根率均为100%, 以20 mg L-1 IBA处理的平均生根数最多。促进少花龙葵半叶硬枝插条生根的IBA浓度为15~35 mg L-1,其中以25 mg L-1 IBA的效果最好。少花龙葵嫩枝和硬枝插条分别在20与25 mg L-1 IBA处理下,均以半叶插条的生根效果最好、缺叶插条的生根效果最差,全叶插条的生根效果居中。 相似文献
8.
外源激素对四倍体刺槐硬枝扦插生根及其关联酶活性的影响 总被引:10,自引:0,他引:10
取四倍体刺槐3年生采穗圃中当年生枝条为插穗,研究了外源激素种类、浓度、处理时间对硬枝插穗生根的影响及生根过程中3种关联酶吲哚乙酸氧化酶(IAAO)、过氧化物酶(POD)和多酚氧化酶(PPO)活性变化规律,以揭示四倍体刺槐插穗生根难的机理.结果显示:(1)四倍体刺槐硬枝插穗生根过程可分愈伤组织诱导期(0~20d)、根源基诱导期(20~30 d)、不定根表达与伸长期(30~45 d)3个阶段.(2)未经外源激素处理的插穗不生根,而激素处理可显著提高插穗生根率,促进根系发育,且IBA处理效果优于GGR6;激素处理浓度与时间无显著互作效应,但插穗生根综合效果随着处理时间和浓度的增加而升高;插穗的平均生根量和生根率在1 000 mg?L-1IBA处理6 h时达到最高,分别为11.74和55.56%.(3)IAAO、PPO是影响四倍体刺槐生根的关键酶,POD影响较小;外源激素处理插穗内IAAO和PPO活性呈先升高后下降的趋势,且均在根源基诱导期达到峰值;对照插穗内IAAO活性峰值出现在愈伤组织诱导期,而其PPO活性一直降低;激素处理和对照插穗内POD活性随着处理时间持续升高.研究表明,外源IBA和GGR6处理均可明显改变与四倍体刺槐插穗生根密切关联的氧化酶IAAO和PPO活性,提高生根率,促进根系发育,且IBA处理效果优于GGR6. 相似文献
9.
棉纤维初始发育过程中过氧化物酶和吲哚乙酸氧化酶的活性 总被引:8,自引:1,他引:8
选用互为近等基因系的陆地棉品种徐州142和无絮棉突变体,比较研究开花前5d至开花后6d胚珠内可溶性蛋白质含量,POD和IAA氧化酶活性的变化,结果表明,开花前3d至1d蛋白质大量合成可能与纤维发育相关基因的表达有联系,而POD和IAA氧化酶阻碍纤维的初始发育。 相似文献
10.
光叶楮扦插生根的吲哚乙酸氧化酶、多酚氧化酶、过氧化物酶活性变化研究 总被引:18,自引:0,他引:18
对光叶楮扦插生根过程中吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)、过氧化物酶(POD)3种酶进行了动态跟踪分析。结果表明:IAAO活性在扦插初期逐渐上升,第10d上升到高峰,之后下降再上升,第30d达到新高峰,然后迅速下降;前25d POD活性变化规律与IAAO相似,但30d以后活性一直上升;PPO活性在扦插前期缓慢上升,第20d上升到了最高点,此后变化不大。还研究了IAAO、PPO、POD与不定根的发生和发展关系,认为光叶楮扦插生根可分为愈伤组织形成期、根诱导期和根的伸长期3个阶段,愈伤组织形成期3种酶活性都呈上升趋势,根诱导期IAAO和POD的活性达到高峰;而根伸长期IAAO和POD活性下降,PPO活性上升。 相似文献
11.
Rooting in terminal shoot and lateral shoot cuttings from 10-year-old elite trees of Casuarina equisetifolia L. in different sex groups was achieved after 20 days when the basal ends of the cuttings were dipped for 3 h in 20 ppm indole-3-butyric acid (IBA). Shoots derived from male plants rooted better than their female and monoecious counterparts, and the lateral shoots were more responsive to rooting than the terminal shoots. During rooting, the metabolic activities varied in both lateral shoot and terminal shoot cuttings derived from plants under different sex groups. Peroxidase and polyphenoloxidase activities were high during root initiation and showed a sharp decline thereafter. The polyphenoloxidase activity was higher in the lateral shoot than the terminal shoot cuttings. The rooted plantlets survived and established well in the field.Abbreviations IAA indole-3-acetic acid - IBA indole-3-butyric acid - NAA 1-naphthaleneacetic acid - PVP polyvinylpyrrolidone 相似文献
12.
Ascorbic acid is a strong inhibitor of indole-3-acetic oxidation catalyzed by commercial horse-radish peroxidase. In the presence of excess ascorbic acid, the indole-acetic acid oxidation catalysis is apparently blocked. The activity of peroxidase for indoleacetic acid at pH 3.7 and 33°C, in the presence of 2,4-dichlorophenol and MnCl2 as promotors was measured by polarographic technique. The Km was 0.27 m M and the maximum velocity was 1.02 mmol O2 (mg protein)−1 min−1 . Dixon plots lead to an apparent Ki of 1.25 (μ M for ascorbic acid and the inhibition was apparently competitive. Ascorbic acid, besides appearing to be a strong inhibitor of the IAA oxidase activity of peroxidase, seemed to protect IAA from total degradation. Addition of more than 5 μ M ascorbic acid produced both an exponential increase in the lag time before the onset of reaction and, at the end, an oxidation protection of 26 μ M IAA when 111 μ M IAA was present at the stawrt. The possibility of ascorbic acid-IAA auxin from endogenous oxidation in plants, is proposed. 相似文献
13.
The influence of indole-3-acetylaspartic acid (IAAsp) on rooting of stem cuttings from bean plants (Phaseolus vulgaris L.) of different ages, cultivated at different temperatures (17°, 21° and 25°C) was studied and compared to that of indole-3-acetic acid (IAA). At a concentration of 10–4 M, IAAsp only nonsignificantly stimulated adventitious root formation, approximately to the same level as IAA in all treatments. IAAsp at 5×10–4 M further enhanced rooting, by up 200% of control values, with little influence of temperature conditions and stock plant age. This concentration of IAA usually stimulated rooting more than the conjugate. The largest differences between the effects of IAAsp and IAA occured at the highest cultivation temperature of 25°C where stock plant age also influenced the response. The number of roots produced in comparison with the control, was enhanced from 350% on cuttings from the youngest plants to more than 600% on cuttings from the oldest. In contrast to the conjugate, 5×10–4 M IAA induced hypocotyl swelling and injury of the epidermis at the base of cuttings, in all treatments. 相似文献
14.
High irradiance during treatment of mung bean cuttings favours root formation in response to supplied auxin, whether the latter is IAA or IBA. On the other hand it is inhibitory towards root formation in the absence of supplied auxin. Light promotes the uptake of14C-IAA into cuttings and its upward movement into the leaves. When14C-IAA is applied to leaves of cuttings high irradiance favours movement of radioactivity into the epicotyl and hypocotyl. This movement is also enhanced by concomitant supply of IBA to the base of the cuttings. The irradiance under which stock plants are raised also affects the extent of root formation on cuttings. When cuttings are held in darkness without a supply of exogenous auxin they root best if prepared from seedlings raised under high irradiance. However, transport of14C-IAA out of leaves of cuttings is favoured when cuttings are prepared from seedlings grown under low irradiance. These observations are discussed in relation to auxin transport, photodestruction and, possibly, metabolism. 相似文献
15.
Fernanda C. Koyama Thais L. G. Carvalho Eduardo Alves Henrique B. da Silva Mauro F. de Azevedo Adriana S. Hemerly Célia R. S. Garcia 《The Journal of eukaryotic microbiology》2013,60(6):646-651
Indole compounds are involved in a range of functions in many organisms. In the human malaria parasite Plasmodium falciparum, melatonin and other tryptophan derivatives are able to modulate its intraerythrocytic cycle, increasing the schizont population as well as parasitemia, likely through ubiquitin‐proteasome system (UPS) gene regulation. In plants, melatonin regulates root development, in a similar way to that described for indoleacetic acid, suggesting that melatonin and indoleacetic acid could co‐participate in some physiological processes due to structural similarities. In the present work, we evaluate whether the chemical structure similarity found in indoleacetic acid and melatonin can lead to similar effects in Arabidopsis thaliana lateral root formation and P. falciparum cell cycle modulation, as well as in the UPS of gene regulation, by qRT‐PCR. Our data show that P. falciparum is not able to respond to indoleacetic acid either in the modulation of the intraerythrocytic cycle or in the gene regulation mediated by the UPS as observed for melatonin. The similarities of these indole compounds are not sufficient to confer synergistic functions in P. falciparum cell cycle modulation, but could interplay in A. thaliana lateral root formation. 相似文献