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1.
T. S. Sonstegard N. L. Lopez-Corrales S. M. Kappes R. T. Stone S. Ambady F. A. Ponce de Leon C. W. Beattie 《Mammalian genome》1997,8(1):16-20
Genotypic data for 56 microsatellites (ms) generated from maternal full sib families nested within paternal half sib pedigrees
were used to construct a linkage map of the bovine X Chromosome (Chr) (BTX) that spans 150 cM (ave. interval 2.7 cM). The
linkage map contains 36 previously unlinked ms; seven generated from a BTXp library. Genotypic data from these 36 ms was merged
into an existing linkage map to more than double the number of informative BTX markers. A male specific linkage map of the
pseudoautosomal region was also constructed from five ms at the distal end of BTXq. Four informative probes physically assigned
by fluorescence in situ hybridization defined the extent of coverage, confirmed the position of the pseudoautosomal region
on the q-arm, and identified a 4.1-cM marker interval containing the centromere of BTX.
Received: 14 July 1996 / Accepted: 19 September 1996 相似文献
2.
A genetic and physical map of bovine chromosome 3 总被引:2,自引:0,他引:2
D Vaiman A Eggen D Mercier I Bahri-Darwich C Grohs D Bruneau P Laurent B Chaput A Oustry G Frelat H Levéziel E P Cribiu 《Animal genetics》1995,26(1):21-25
This paper reports a map of nine polymorphic microsatellite markers previously assigned to bovine chromosome 3 (BTA3) by somatic cell genetics. The linkage group covers 101 cM on the chromosome with an average intermarker distance of 13-9 cM. One marker (INRA200) was isolated from a peak of flow sorted chromosomes 2 and 3. Another marker (INRA197) was derived from a cosmid. The localization of the cosmid by in situ hybridization enabled the orientation of the linkage group on BTA3. Markers were relatively evenly spaced and consequently can be used to complement other mapping data about this chromosome. This establishes a framework of polymorphic markers that can be used to search for quantitative trait loci (QTL). 相似文献
3.
A male-specific genetic linkage map of nine loci on bovine Chromosome (Chr) 2 (BTA2) was constructed from 306 offspring belonging to six paternal halfsib families. Loci studied were the structural genes for liver/bone/kidney alkaline phosphatase (ALPL), Gardner-Rasheed feline sarcoma (v-fgr) oncogene homolog (FGR), alpha-L-fucosidase 1 (FUCA1), and fibronectin 1 (FN1), and the microsatellite loci ARO28, DU17S2, DU17S3, DU17S4, and DU17S5. Genotyping was performed by restriction fragment length polymorphism (RFLP) for structural genes and polymerase chain reaction (PCR) for the microsatellites. Two genetically independent linkage groups were identified. The order of genes in the first linkage group, L31, is (ARO28-FN1)-FGR-FUCA1-ALPL, covering a map distance of 34.1 cM between terminal markers. The second linkage group, L32, consists of DU17S2-DU17S5-DU17S4-DU17S3 and is 41.3 cM in length. Genetic linkage between FN1 and FGR confirms previous physical assignment of these genes to the same synteny group. Currently, the genetic linkage of FN1 and FGR is unique to cattle and thus localizes a site of chromosomal evolution to a 22-cM interval between the two loci. 相似文献
4.
5.
L. Ferretti B. G. D. Urquhart A. Eggen I. Olsaker B. Harlizius B. Castiglioni A. Mezzelani S. Solinas Toldo U. Thieven Y. Zhang A. L. G. Morgan V. M. Teres M. Schwerin I. Martin-Burriel B. P. Chowdhary G. Erhardt I. J. Nijman E. P. Cribiu W. Barendse H. Leveziel R. Fries J. L. Williams 《Mammalian genome》1997,8(1):29-36
The mapping strategy for the bovine genome described in this paper uses large insert clones as a tool for physical mapping
and as a source of highly polymorphic microsatellites for genetic typing, and was one objective of the BovMap Project funded
by the European Union (UE). Eight-three cosmid and phage clones were characterized and used to physically anchor the linkage
groups defining all the bovine autosomes and the X Chromosome (Chr). By combining physical and genetic mapping, clones described
in this paper have led to the identification of the linkage groups corresponding to Chr 9, 12, 16, and 25. In addition, anchored
loci from this study were used to orient the linkage groups corresponding to Chr 3, 7, 8, 9, 13, 16, 18, 19, and 28 as identified
in previously published maps. Comparison of the estimated size of the physical and linkage maps suggests that the genetic
length of the bovine genome may be around 4000 cM.
Received: 1 July 1996 / Accepted: 13 September 1996 相似文献
6.
A genetic linkage map of bovine Chromosome (Chr) 7 was generated with a Bos taurus×Bos gaurus interspecific hybrid backcross panel. This study included six previously mapped microsatellites and five unmapped expressed
genes that were identified by PCR-based restriction fragment length variants (RFLVs). The gene order (from centromere to telomere)
and the map distances (in centimorgans) are as follows: cen–BM2607–11.2–LDLR–3.6–AMH,CSF2–11.2–BP41–19–BM6117–19–SPARC–14.4–FGFA–15.5–BM1853–11.2–RASA–18.8–ILSTS006. Previous comparative synteny mapping demonstrated that bovine Chr 7 shares homologous regions with both HSA5q and HSA19p.
A break or fusion between AMH and CSF2 in an ancestral chromosome is suggested to account for the current arrangement of these homologous segments in the human
and bovine genomes. In this study, we demonstrate that a short proximal portion of BTA7 is homologous with HSA19p, while a
larger distal portion of BTA7 is homologous with human Chr 5q. The orientation of these conserved human segments on BTA7 is
also demonstrated. Our data show that the linear order of genes has not been conserved within the homologous region of HSA5
and BTA7, and one chromosomal translocation or inversion is proposed to account for this difference.
Received: 11 June 1996 / Accepted: 9 November 1996 相似文献
7.
An extensive and comprehensive radiation hybrid map of bovine Chromosome 15 (BTA15) was built with 42 anonymous markers,
3 ESTs, and 49 genes. This work allows us to refine the comparative map between human Chromosome (Chr) 11 (HSA11) and BTA15.
Four blocks with a similar gene content and relatively good gene order conservation were identified. The discrepancies are
concentrated on closely positioned genes for which discrimination is not possible between mapping resolution limits in either
the human or the bovine maps and true local inversions. Using the gene order similarity and the human physical map as starting
point, we estimated the overall physical length of BTA15 to be around 75.3 Mb. The INRA bovine BAC library was screened for
all the markers ordered on the bovine map, which will provide anchors for future efforts in the construction of a physical
map of the bovine genome.
Finally, this map contains the majority of publicly available polymorphic markers described for BTA15 and integrates those
with comparative mapping information. It should, therefore, constitute a powerful tool in the identification of relevant candidate
genes in regions of BTA15 harboring economic trait loci. 相似文献
8.
Snelling WM Chiu R Schein JE Hobbs M Abbey CA Adelson DL Aerts J Bennett GL Bosdet IE Boussaha M Brauning R Caetano AR Costa MM Crawford AM Dalrymple BP Eggen A Everts-van der Wind A Floriot S Gautier M Gill CA Green RD Holt R Jann O Jones SJ Kappes SM Keele JW de Jong PJ Larkin DM Lewin HA McEwan JC McKay S Marra MA Mathewson CA Matukumalli LK Moore SS Murdoch B Nicholas FW Osoegawa K Roy A Salih H Schibler L Schnabel RD Silveri L Skow LC Smith TP Sonstegard TS Taylor JF Tellam R 《Genome biology》2007,8(8):R165
Background
Cattle are important agriculturally and relevant as a model organism. Previously described genetic and radiation hybrid (RH) maps of the bovine genome have been used to identify genomic regions and genes affecting specific traits. Application of these maps to identify influential genetic polymorphisms will be enhanced by integration with each other and with bacterial artificial chromosome (BAC) libraries. The BAC libraries and clone maps are essential for the hybrid clone-by-clone/whole-genome shotgun sequencing approach taken by the bovine genome sequencing project.Results
A bovine BAC map was constructed with HindIII restriction digest fragments of 290,797 BAC clones from animals of three different breeds. Comparative mapping of 422,522 BAC end sequences assisted with BAC map ordering and assembly. Genotypes and pedigree from two genetic maps and marker scores from three whole-genome RH panels were consolidated on a 17,254-marker composite map. Sequence similarity allowed integrating the BAC and composite maps with the bovine draft assembly (Btau3.1), establishing a comprehensive resource describing the bovine genome. Agreement between the marker and BAC maps and the draft assembly is high, although discrepancies exist. The composite and BAC maps are more similar than either is to the draft assembly.Conclusion
Further refinement of the maps and greater integration into the genome assembly process may contribute to a high quality assembly. The maps provide resources to associate phenotypic variation with underlying genomic variation, and are crucial resources for understanding the biology underpinning this important ruminant species so closely associated with humans. 相似文献9.
10.
Laurent Schibler Anne Roig Marie-Fran?oise Mahé Jean-Claude Save Mathieu Gautier Sead Taourit Didier Boichard André Eggen Edmond P Cribiu 《遗传、选种与进化》2004,36(1):105-122
A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest. 相似文献
11.
A radiation hybrid map for the bovine Y Chromosome 总被引:7,自引:0,他引:7
Wan-Sheng Liu Paola Mariani Craig W. Beattie Leeson J. Alexander F. Abel Ponce de León 《Mammalian genome》2002,13(6):320-326
Screening a bovine Y Chromosome-specific DNA library resulted in 34 new microsatellites, six of which mapped to the pseudoautosomal
region (PAR), and 28 localized to the Y-specific region. These microsatellites, together with 23 markers previously mapped
to the bovine Y Chr, were scored on a 7000-rad cattle–hamster radiation hybrid (RH) panel. Retention frequency of individual
markers ranged from 18.5% to 76.5% with an average of 48.4%. Markers with high retention frequency (>55%) were found to exist
in multiple copies on the Y Chr. Thirteen markers were placed on the PAR RH map with the AmelY gene proximal to the pseudoautosomal boundary and 46 markers, including Sry and Tspy gene, on the Y-specific region of the RH map. The microsatellites developed and mapped in this work will be useful for comparative
mapping of cattle, sheep, and goat, studying the origin, evolution, and migration of bovidae species and provide an initial platform to develop a high-resolution map of the Y Chr and positional cloning of Y-specific
genes. 相似文献
12.
13.
Y. Du E. F. Remmers H. Zha E. A. Goldmuntz J. Szpirer C. Szpirer R. L. Wilder 《Mammalian genome》1995,6(4):295-298
Nine microsatellite loci were mapped to rat Chromosome (Chr) 7 by genetic linkage and somatic cell hybrid analysis. These loci include the gene encoding a member of the IID sub-family of cytochrome P450 (Cyp2d), a gene with repetitive sequences expressed during myotube formation (D7Arb1e), four anonymous loci, D7Arb81, D7Arb208, D7Arb569, D7Arb609a, and three DNA loci defined by MapPairTM markers R245, R513, and R1071. The nine loci were all identified by PCR-based microsatellite polymorphism analysis and were characterized in 40 F2 intercross progeny of Fischer (F344/N) and Lewis (LEW/N) rats for segregation analysis. These markers formed a single linkage group spanning 76.8 cM with the following order and distances: D7Arb569-11.4 cM-D7Arb81-9.7 cM-R513-2.6 cM-Cyp2d-0.0 cM-R245-1.3 cM-D7Arb1e-10.4 cM-R1071-15.9 cM-D7Arb609a-15.4 cM-D7Arb208. Physical mapping of Cyp2d by somatic cell hybrid analysis allowed us to assign this linkage group to rat Chr 7. For each marker, two to six alleles were detected in a panel of 16 inbred rat strains (ACI/N, BN/SsN, BUF/N, DA/Bkl, F344/N, LER/N, LEW/N, LOU/MN, MNR/N, MR/N, SHR/N, SR/Jr, SS/Jr, WBB1/N, WBB2/N, WKY/N). 相似文献
14.
We present a comprehensive radiation hybrid map of the bovine X chromosome (Chr) containing 20 new markers, including both
microsatellites and expressed genes. This study was conducted with a 5000-rad whole genome RH cell panel consisting of 90
hybrid cell lines. Retention frequencies of individual markers range from 7.8% for XIST to 31.1% for TGLA325. Statistical
analysis with RHMAPPER placed all the loci into five linkage groups under a LOD score criterion of 6.0. These groups could
be oriented relative to each other because they included multiple microsatellite loci from the consensus linkage map of the
X Chr. Markers included in both this RH map and the bovine cytogenetic map were in a consistent order. The comparative bovine–human
map thus generated consists of five blocks of genes, the order of which is conserved, although in the opposite direction when
presented as ideograms with p and q arms. Inversions of three blocks account for the difference in gene order across the entirety
of the two X Chrs. 相似文献
15.
A medium-density genetic linkage map of the bovine genome 总被引:22,自引:0,他引:22
W. Barendse D. Vaiman S. J. Kemp Y. Sugimoto S. M. Armitage J. L. Williams H. S. Sun A. Eggen M. Agaba S. A. Aleyasin M. Band M. D. Bishop J. Buitkamp K. Byrne F. Collins L. Cooper W. Coppettiers B. Denys R. D. Drinkwater K. Easterday C. Elduque S. Ennis G. Erhardt L. Ferretti N. Flavin Q. Gao M. Georges R. Gurung B. Harlizius G. Hawkins J. Hetzel T. Hirano D. Hulme C. Jorgensen M. Kessler B. W. Kirkpatrick B. Konfortov S. Kostia C. Kuhn J. A. Lenstra H. Leveziel H. A. Lewin B. Leyhe L. Lil I. Martin Burriel R. A. McGraw J. R. Miller D. E. Moody S. S. Moore S. Nakane I. J. Nijman I. Olsaker D. Pomp A. Rando M. Ron A. Shalom A. J. Teale U. Thieven B. G. D. Urquhart D. -I. Vage A. Van de Weghe S. Varvio R. Velmala J. Vilkki R. Weikard C. Woodside J. E. Womack M. Zanotti P. Zaragoza 《Mammalian genome》1997,8(1):21-28
A cattle genetic linkage map was constructed which covers more than 95 percent of the bovine genome at medium density. Seven
hundred and forty six DNA polymorphisms were genotyped in cattle families which comprise 347 individuals in full sibling pedigrees.
Seven hundred and three of the loci are linked to at least one other locus. All linkage groups are assigned to chromosomes,
and all are orientated with regards to the centromere. There is little overall difference in the lengths of the bull and cow
linkage maps although there are individual differences between maps of chromosomes. One hundred and sixty polymorphisms are
in or near genes, and the resultant genome-wide comparative analyses indicate that while there is greater conservation of
synteny between cattle and humans compared with mice, the conservation of gene order between cattle and humans is much less
than would be expected from the conservation of synteny. This map provides a basis for high-resolution mapping of the bovine
genome with physical resources such as Yeast and Bacterial Artificial Chromosomes as well as providing the underpinning for
the interpolation of information from the Human Genome Project.
Received: 15 August 1996 / Accepted: 15 September 1996 相似文献
16.
A physical and genetic map of Neisseria meningitidis B1940 总被引:2,自引:0,他引:2
Martin Gäher Kerstin Einsiedler Torsten Crass & Wilfried Bautsch 《Molecular microbiology》1996,19(2):249-259
A physical map of the chromosome of Neisseria meningitidis B1940 has been constructed by one- and two-dimensional pulsed-field gel electrophoresis techniques. Complete macrorestriction maps for the enzymes Nhe I (16 sites), Sgf I (13 sites), Sfi I (11 sites) and I-Ceu I (4 sites), as well as a partial restriction map for the restriction enzyme Spe I (15 of c. 28 sites) could be established. Altogether, 59 restriction sites were mapped on a single circular chromosome of 2.3 Mbp. By restriction endonuclease digestion and Southern hybridization of cloned genetic markers, 39 genetic loci were assigned to this map. Comparison with the metabolic maps of Neisseria gonorrhoeae MS11-N198 and FA1090 revealed a high degree of conservation in the arrangement of gene loci among these two species, although four out of 24 genetic loci are located at different chromosomal positions, indicating several genomic rearrangements. 相似文献
17.
A combined physical and genetic map of Pseudomonas aeruginosa PAO 总被引:27,自引:0,他引:27
E Ratnaningsih S Dharmsthiti V Krishnapillai A Morgan M Sinclair B W Holloway 《Journal of general microbiology》1990,136(12):2351-2357
A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome. 相似文献
18.
19.
P. A. Baldacci M. Cohen-Tannoudji C. Kress S. Pournin C. Babinet 《Mammalian genome》1996,7(2):114-116
The locus Om (ovum mutant) identified in the mouse strain DDK affects the viability of (DDK |m~ non-DDK)F1 preimplantation embryos. We previously located this locus on Chromosome (Chr) 11 close to Scya2 (Baldacci et al. Mamm. Genome 2, 100–105, 1992). Here we report a high-resolution map of the region around Om based on a large number of backcross individuals. The same region has been analyzed on the EUCIB backcross, and the two maps
have been compared. The results define the proximal and distal boundaries for the Om mutation as Scya2 and D11Mit36 respectively. The distance between these two markers is about 2 cM. These data should facilitate the positional cloning and
molecular characterization of Om.
Received: 10 July 1995 / Accepted: 11 September 1995 相似文献
20.
J. Riquet D. Milan N. Woloszyn A. Schmitz F. Pitel G. Frelat J. Gellin 《Mammalian genome》1995,6(9):623-628
We have developed a simple and efficient method to construct partial libraries of swine Chromosome (Chr) 11, starting with only 300 flow-sorted copies. DNA is amplified by PARM-PCR with primer containing at the 5-end the sequence AGCU-. After amplification, digestion of PCR products with uracil DNA glycosylase generates cohesive ends corresponding to the SstI site. The amplified fragments can then be ligated in vector linearized with the SstI enzyme. Using five different primers, we PARM-PCR amplified and cloned swine Chr 11 DNA. These chromosome-specific libraries have been used to develop 14 different (TG)n microsatellites. Ten of these markers were assigned to Chr 11 by PCR analysis of a panel of Pig-Rodent somatic hybrids and by linkage analysis of the 171 individuals of the PiGMaP reference families. A complete linkage map of 147 cM of this chromosome was then realized by integrating existing markers. 相似文献