十二种淡水鱼类血红蛋白与窒息点的相关分析

为了解鱼类耐低氧能力与血红蛋白的相关性,测定了大口鲇(Silurus meridionalis)、乌鳢(Channa argus)、加洲鲈(Micropterus salmoides)、斑点叉尾(Ictalurus punetaus)、黄颡鱼(Pelteobagrus fulvidraco)、泥鳅(Misgurnus anguillicaudatus)、黄鳝(Monopterus albus)、草鱼(Ctenopharyngodon idellus)、翘嘴红鲌(Erythroculter ilishaeformis)、鲢(Hypophthalmichthys molitrix)、鳙(Aristichthys nobilis)和鳜(Siniperca chuatsi)12种鱼的窒息点,同时分别对其血红蛋白进行电泳分析。12种鱼的血红蛋白电泳图谱各不相同,大口鲇、乌鳢、加洲鲈、斑点叉尾、黄颡鱼、泥鳅、黄鳝、草鱼、翘嘴红鲌、鲢、鳙、鳜的血红蛋白分子量加权平均值分别为92.93、93.91、94.61、97.78、98.66、98.95、101.34、104.10、104.73、108.06、108.23、111.84 ku,窒息点分别为0.15、0.18、0.30、0.27、0.36、0.26、0.24、0.39、0.42、0.51、0.46、0.59 mg/L。分析发现,这12种鱼类的窒息点与血红蛋白分子量呈极显著正相关(r=0.925,P0.01)。

Correlation between Hemoglobin and Asphyxiation Point in Twelve Species of Freshwater Fish

To understand the correlation between the hypoxia tolerance ability and the hemoglobin (Hb) in fish. A total of 12 different species of fish, Catfish (Silurus meridionalis), Snakehead (Channa argus), California Perch (Micropterus salmoides), Channel Catfish (Ictalurus punetaus), Yellow Catfish (Pelteobagrus fulvidraco), Loach (Misgurnus anguillicaudatus), Eel (Monopterus albus), Grass Carp (Ctenopharyngodon idellus), Erythroculter Ilishaeformis (Erythroculter ilishaeformis), Silver Carp (Hypophthalmichthys molitrix), Big Head Carp (Aristichthys nobilis) and Chinese Perch (Siniperca chuatsi), were collected from Luhu Fishery and suburban of Wuhan. The hemoglobin was extracted from blood of each species separately. By using vertical polyacrylamide gel electrophoresis technology, the hemoglobin of each species was analyzed. Compared to logarithm of the standard protein molecular weight and mobility of standard curve, the mobility and standard curve of hemoglobin molecular weight was obtained after electrophoresis. With gel image analysis software Bandscan V5.0, the relative content of each hemoglobin band was measured, and the average hemoglobin molecular weight was calculated for each fish. The suffocation points of 12 species fish were determined by closed hydrostatic device. The results showed that the hemoglobin electrophoretograms were not identical (Fig. 1). Their hemoglobin molecular weights were: 92.93, 93.91, 94.61, 97.78, 98.66, 98.95, 101.34, 104.10, 104.73, 108.06, 108.23, 111.84 (ku), respectively, and their asphyxiation points were 0.15, 0.18, 0.30, 0.27, 0.36, 0.26, 0.24, 0.39, 0.42, 0.51, 0.46 and 0.59 (mg/L), respectively (Table 3). The correlation analysis found that asphyxiation point and the hemoglobin molecular weight were significantly positive correlated (r = 0.925, P < 0.01).