黑龙江草蜥消化道5-羟色胺免疫活性内分泌细胞的分布与形态学观察

应用5-HT抗血清，以ABC(avidin-biotin-peroxidase complex)免疫组织化学方法，对黑龙江草蜥(Takydromus amurensis)消化道内5-HT免疫反应阳性内分泌细胞的分布及形态进行了观察。结果显示：5-HT阳性细胞从食管到直肠的消化道各段均有分布。细胞分布密度呈波浪式，食管、胃幽门部和回肠是其细胞分布密度的高峰。5-HT阳性细胞的形态呈圆形、椭圆形、锥体形、梭形等，其中以圆形和椭圆形为主；广泛分布于上皮基部、上皮细胞之间、腺泡上皮及腺泡之间，有时可见于固有膜内。因此作者认为5-HT阳性细胞具有内、外、旁分泌三种作用途径并且它的密度分布可能与其食性、生活环境等有关。

Distribution and Morphological Observation of 5-HT Positive Immunoreactive Endocrine Cells in Digestive Tract of Takydromus amurensis

The presence and morphology of 5 HT positive, immunoreactive endocrine cells in the digestive tract of Takydromus amurensis have been investigated by using the anti 5 HT antiserum and the ABC immunostaining technique. The results indicated that 5 HT positive cells distributed throughout the digestive tract, from esophagus to rectum. The cells' distribution density was not consistent along the digestive tract, and there were rich 5 HT cells in the esophagus, pyloric part and ileum. The 5 HT cells was round, ellipse, shuttle, or cone in shape. The cells were found between gastrointestinal epithelial cells, at the basal region of epithelial cells, between glandular epithelial cells, between glandular vesicles and sometimes in the lamina propria.We suggest that the distribution of 5 HT positive immunoreactive endocrine cells is related to the feeding habit and life environment of T.amurensis, and they might have endocrine, exocrine, and paracrine functions.