河蚌培养组织的几种生化成分分析

本文分析测定了三角帆蚌，褶纹冠蚌和背角无齿蚌外套膜培养组织及其培养液中的氨基酸，牛磺酸及钙含量。在珍蛛中含量较高的丙的氨酸和甘氨酸分别增加５４１％和９１％。三种蚌在培养中牛磺酸含量增加５．７８％到３倍，培养组织的钙含量增加１倍左右。同时测定了培养组织的碱性磷酸酶活性，培养组织与河蚌外套膜具有相近的比活及相对酶活。结果表明，河蚌外套膜在离体培养条件下，也具有分泌珍珠质的能力。

ANALYSIS ON SEVERAL BIOCHEMICAL COMPONENT OF THE CULTURED TISSUE OF FRESH WATER OYSTER

Content analyses were made for amino acid, taurine, and calcium which are existed in the cultured tissue and cultured solution of the mantle of fresh water oyster (Hyriopsis cumiugii Lea, Cristaria plicata Leach, Anodonta woodiana Lea). These components can be seen in nature pearls. The contents of Alanine and Glycine were higher in the culture solution than in the control culture solution by 54l% and 91% respectively.The contents of taurine in the culture solution with three fresh water oysters were found to increase 5.78% to 300% than those in the control one, calcium in the cultured tissues was increased about 1 time than that in the control solution too. The specific and relative activities of alkaline phosphatase were similar for both nature and cultured tissues of mantles in this experiment. The results showed that the mantle cells cultured in artificial condition can secret materials which are naturally produced by nacres.