鲫幼鱼耳石荧光标记效果

为确认茜素络合物对鲫（Carassius auratus）耳石进行有效标记的可行性，以便为鲫甚至其他鲤科鱼类标志放流技术的开发及效果评估提供一定的借鉴，本研究以孵出后90 d的鲫幼鱼为研究对象，设置单一浓度（100 mg/L）的茜素络合物浸泡标记5 d，分析茜素络合物在耳石上的沉积情况以及不同耳石在不同后续饲养天数的动态变化。结果表明，矢耳石、微耳石和星耳石上在可见光、绿色和蓝色激发光下都检测到了良好的茜素络合物标记环，标记率和存活率均为100%。但不同耳石的茜素络合物标记效果不同，荧光下，星耳石的标记效果最显著，微耳石次之；可见光下，微耳石的标记效果最好，星耳石次之。随着后续饲养天数的延长，可见光下标记逐渐减弱，至20 d时基本消失，而在绿色和蓝色激光下标记环荧光强度无减弱迹象，能长久保持，且在蓝色激发光下标记环更易被观测到。上述结果结合鲫生长、存活和行为正常等情况综合显示，在100 mg/L茜素络合物溶液中浸泡标记鲫幼鱼5 d，其耳石可以获得满意的标记效果。

Effect of Alizarin Complexone Marking for Otolith of Juvenile Crucian Carp Carassius auratus

For assessing the feasibility of otolith alizarin complexone (ALC) marking of crucian carp Carassius auratus and providing a useful reference to develop batch marking technologies for the release and enhancement of this species or even other cyprinidae fish, juvenile C. auratus (Fig. 1) were used to investigate the deposition of ALC onto otolith by immersing it in 100 mg/L for 5 d and the dynamic changes of ALC markings among three types of otoliths at different days of subsequent culture after the immersion. The results showed that ALC marking rings were detected on the sagittal, lapillus and asteriscus under visible light, green and blue excitation light with a marking rate of 100% without extra impact on survival and growth of the fish (Fig. 2). However, the marking effect of different otoliths was different. Under fluorescence, asteriscus showed the best marking effect, followed by lapillus, while under visible light, lapillus had the best marking effect, followed by asteriscus (Fig. 3). With the extension of the days of the subsequent culture, the visible light marker gradually weakened and almost disappeared at 20 d. However, under the green and blue excitation light, the fluorescence intensity of the ALC marking ring kept almost stable and was maintained for a long time (Table 1). Moreover, the marker ring could be easily observed under blue excitation light (Fig. 4). In combination with data of mortality, total length and body weight, it is believed that immersion by 100 mg/L ALC for 5 d can achieve satisfactory effect for otolith marking of juvenile C. auratus.