AFLP标记在小香羊遗传多态性检测中的应用 |
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引用本文: | 苟本富,叶华虎,魏泓,邹国林.AFLP标记在小香羊遗传多态性检测中的应用[J].动物学杂志,2003,38(5):45-49. |
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作者姓名: | 苟本富 叶华虎 魏泓 邹国林 |
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作者单位: | 1. 武汉大学生命科学学院,武汉,430072;渝西学院生命科学系,重庆,402168 2. 第三军医大学实验动物中心,重庆,400038 3. 武汉大学生命科学学院,武汉,430072 |
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基金项目: | 国家自然科学基金,30070120, |
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摘 要: | 研究了AFLP标记在研究小香羊遗传多态性方面应用的可行性和该山羊个体基因组DNA的AFLP扩增结果。实验应用10条AFLP引物,用PstI酶切,对15只小香羊基因组DNA进行AFLP反应,共获得113个AFLP标记,单引物获得的标记数在2~19之间,小香羊群体相似系数AFLP研究结果为0.913(0.814~0.980)。该研究为评价小香羊的遗传稳定性提供了相关的参数,准确评价尚待和其它品种对比研究后确定。
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关 键 词: | 小香羊 遗传多态性 遗传相似系数 AFLP |
文章编号: | 0250-3263(2003)05-45-05 |
Application of AFLP in Genetic Polymorphism Monitoring of Miniature Xiang Goats |
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Abstract: | The AFLP markers were applied to detect genetic polymorphic loci in miniature Xiang goats.Ten AFLP primers were used, and genomic DNA from 15 goats were analyzed. A total of 113 AFLP markers were obtained, and the marker numbers of individual primer were between 2 to 19.The results indicate that the AFLP marker is suitable for analyzing genetic polymorphism in goat and that(2) the similarity index of population in miniature Xiang goats was 0 913(0 814 to 0 980). |
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Keywords: | Miniature Xiang goat Genetic polymorphism Genetic similarity coefficient AFLP (amplified fragment length polymorphism) |
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