濒危高原植物羌活化学成分与生态因子的相关性

羌活（Notopterygii Rhizoma Et Radix） 是伞形科植物羌活（N. incisum）或宽叶羌活（N. franchetii）的干燥根茎和根，为濒危高原药用植物。考察羌活主产地生态气象因子对其主要化学成分含量的影响，探讨气象因子与羌活品质的关系。应用HPLC方法检测羌活醇，异欧前胡素2种主要化学成分含量。采用中国气象科学数据共享服务网平台获得不同产地的平均气压、年降水量、平均风速、日照时数、相对湿度、年平均温度、年最高温度、年最低温度等9个生态因子数据，通过偏最小二乘回归分析（PLS）、冗余分析（RDA）分析了其化学成分含量与生态因子之间的相关性。化学分析表明，羌活中羌活醇的含量高于异欧前胡素，而宽叶羌活中羌活醇含量低于异欧前胡素。经PLS及RDA分析，羌活中羌活醇与日照时数呈强正相关关系，其次是年降水量和海拔。异欧前胡素与年降水量呈强负相关关系，其次是温度。宽叶羌活中影响羌活醇含量的主导因子分别是海拔、年降水量，且都呈正相关。同时2个主导因子对异欧前胡素含量的影响呈负显著相关。因此日照时数、海拔、年降水量是影响羌活化学成分累积的主要因素，日照时数有利于羌活化学成分累积，在一定范围内，海拔越高，年降水量越大，越能促进羌活醇的积累，而不利于异欧前胡素含量富集。基于化学成分含量主成分分析（PCA）表明，羌活和宽叶羌活以四川产为最优。研究结果对保护濒危高原植物羌活及引种栽培奠定基础。

Correlative study between chemical constituents and ecological factors of Notopterygii Rhizoma Et Radix of endangered plateau plant

Notopterygii Rhizoma Et Radix, a plateau endangered medicinal herbs, is the rhizoma of umbelliferous plant N. incisum and N. franchetii. This paper aim to investigate the correlation between ecological factors of production area of Notopterygii Rhizoma Et Radix, and the major active components contents. HPLC was established to analyze the contents of two chemicals(Notopterol, Isoimperatorin).Data of 9 ecological factors,including air pressure, annual average temperature, maximum annual temperature, minimum annual temperature,annual precipitation, average wind speed,sunshine duration, and relative humidity, were obtained from the China Meteorological Data Service Network. And then, the relationship of chemical compounds and ecological factors were analyzed Partial Least Squares (PLS) and Redundancy Analysis (RDA) methods. Chemical determination shows the content of Notopterol are higer than Isoimperatorin in N. incisum, but the content of Notopterol are belower than Isoimperatorin in N. franchetii.. Analyses by PLS and RDA, sunshine duration is the most important eco-factor and is positive correlation with Notopterol in N. incisum. And the second and third is annual precipitation and elevation. Annual precipitation is the biggest eco-factor and is negative correlation with Isoimperatorin in N. incisum,Second one is temperature. However, in N. franchetii, altitude, annual precipitation and average wind speed are principal factors that influencing on Notopterol and positively correlation with it. Meanwhile these three main influence factors have significant negative correlation with Isoimperatorin. The result indicated that sunshine duration altitude and annual precipitation are primary factors influencing accumulation of chemical compounds in Notopterygii Rhizoma Et Radix, sunshine duration enhance the accumulation of chemical compounds in Notopterygii Rhizoma Et Radix,the higher altitude,and more annual precipitation means better accumulation of the Notopterol, On the contrary, Isoimperatorin is negative with these two eco-factors. Producing regionalization based on chemical contents by principal component analysis displayed that Sichuan was the best optimal area for N. incisum and N. franchetii.Our data provide scientific proof for protection and cultivation of endangered plateau Notopterygii Rhizoma Et Radix.