Universal primers for the PCR-mediated amplification of DNA β |
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| Authors: | R W Briddon S E Bull S Mansoor I Amin P G Markham |
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| Institution: | (1) Department of Disease and Stress Biology, John Innes Centre, Colney Lane, NR4 7UH Norwich, UK;(2) National Institute of Biotechnology and Genetic Engineering, Jhang Road, P.O. Box 577, Faisalabad, Pakistan |
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| Abstract: | DNA β is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite
geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission,
possibly by trans-encapsidation. Sequence comparisons of the two available DNA β sequences has identified a highly conserved region upstream
of a predicted hairpin structure. Abutting primers designed to this conserved region allows PCR-mediated amplification of
the full-length DNA β component from total nucleic acid extracts isolated from infected plants originating from a variety
of geographically distinct sources and host plants. |
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| Keywords: | Geminivirus satellite whitefly begomovirus DNA β |
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