| Characterization and mapping of a novel mutant sms1 （senescence and male sterility 1） in rice |
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| 作者姓名： | Wenyi Yan Shenghai Ye Qingsheng Jin Longjun Zeng Yu Peng Dawei Yan Weibing Yang Donglei Yang Zuhua He Yanjun Dong Xiaoming Zhang |
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| 作者单位： | [1]College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China [2]Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China [3]Institute of Plant Physiology ＆ Ecology Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China |
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| 基金项目： | supported by the grants from the Science and Technology Office of Zhejiang Province China （No. 2007C12902 and 2008C22068）; Zhejiang Provincial Natural Science Foundation of China Chinese Ministry of Agriculture （No. 200803034）; the Hi-Tech Research and Development Program of China （No. 2007AA100101 and 2008AA02Z125） |
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| 摘 要： | Plant senescence plays diverse important roles in development and environmental responses.However,the molecular basis of plant senescence is remained largely unknown.A rice spontaneous mutant with the character of early senescence and male sterility (sms) was found in the breeding line NT10-748.In order to identify the gene SMS1 and the underlying mechanism,we preliminarily analyzed physiological and biochemical phenotypes of the mutant.The mutant contained lower chlorophyll content compared with the wild type control and was severe male sterile with lower pollen viability.Genetic analysis showed that the mutant was controlled by a single recessive gene.By the map-based cloning approach,we fine-mapped SMS1 to a 67 kb region between the markers Z3-4 and Z1-1 on chromosome 8 using 1,074 F2 recessive plants derived from the cross between the mutant sms1 (japonica) × Zhenshan 97 (indica),where no known gene involved in senescence or male sterility has been identified.Therefore the SMS1 gene will be a novel gene that regulates the two developmental processes.The further cloning and functional analysis of the SMS1 gene is under way.
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| 关 键 词： | 男性不育症 植物衰老 突变体 映射 水稻 表征 雄性不育 分子基础 |
| 收稿时间： | 18 August 2009 |
Characterization and mapping of a novel mutant sms1(Senescence and male sterility 1)in rice |
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| Wenyi Yan,Shenghai Ye,Qingsheng Jin,Longjun Zeng,Yu Peng,Dawei Yan,Weibing Yang,Donglei Yang,Zuhua He,Yanjun Dong,Xiaoming Zhang.Characterization and mapping of a novel mutant sms1(Senescence and male sterility 1)in rice[J].Journal of Genetics and Genomics,2010,37(1):47-55. |
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| Authors: | Wenyi Yan Shenghai Ye Qingsheng Jin Longjun Zeng Yu Peng Dawei Yan Weibing Yang Donglei Yang Zuhua He Yanjun Dong Xiaoming Zhang |
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| Institution: | 1. 2nd Department of Medicine and Cardiology Centre, Medical Faculty, Albert Szent-Györgyi Clinical Centre, University of Szeged, Szeged, Hungary;2. 1st Department of Medicine, Medical Faculty, Albert Szent-Györgyi Clinical Centre, University of Szeged, Szeged, Hungary;3. Department of Pharmacology and Pharmacotherapy, Medical Faculty, Albert Szent-Györgyi Clinical Centre, University of Szeged, Szeged, Hungary;1. Department of Pathophysiology, University of Szeged, Szeged, Hungary;2. MTA-SZTE Neuroscience Research Group of the Hungarian Academy of Sciences Szeged, Szeged, Hungary |
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| Abstract: | Plant senescence plays diverse important roles in development and environmental responses.However,the molecular basis of plant senescence is remained largely unknown.A rice spontaneous mutant with the character of early senescence and male sterility(sms)was found in the breeding line NT10-748.In order to identify the gene SMS1 and the underlying mechanism,we preliminarily analyzed physiological and biochemical phenotypes of the mutant.The mutant contained lower chlorophyll content compared with the wild type control and was severe male sterile with lower pollen viability.Genetic analysis showed that the mutant was controlled by a single recessive gene.By the map-based cloning approach.we fine-mapped SMS1 to a 67 kb region between the markers Z3-4 and Z1-1 on chromosome 8 using 1,074 F_2 recessive plants derived from the cross between the mutant sms1(japonica)×Zhenshan 97(indica),where no known gene involved in senescence or male sterility has been identified.Therefore the SMS1 gene will be a novel gene that regulates the two developmental processes.The further cloning and functional analysis of the SMS1 gene is under way. |
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| Keywords: | senescence male stedlity:rice molecular marker gene mapping |
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