水稻纹枯病抗性QTL分析

对灿稻窄叶青8号（ZYQ8）和粳稻京系17（JX17）以及由它们构建的加倍单倍体（DH）群体，分别在杭州和海南岛，采用注射器接种法进行纹枯病抗性鉴定，并使用该群体的分子链锁图谱进行数量性状座位（QTL）分析。共检测到4个抗纹枯病的QTL（qSBR-2、qSBR-3、qSBR-7和qSBR-11），分别位于第2、第3、第7和第11染色体。其中qSBR-2、qSBR-3、qSBR-7的抗性基因由抗病亲本ZYQ8贡献，而qSBR-11的抗性基因来自感病亲本JX17。qSBR-2、qSBR-3、qSBR-7在杭州和海南岛都能检测到，而qSBR-11只在杭州检测到。在杭州的实验中，纹枯病病级与秆长和抽穗期呈显著负相关；在控制秆长和抽穗期的QTL中，控制秆长的qCL-3与qSBR-3位于同一染色体区域，其余QTL与抗纹枯病的QTL之间无连锁关系。

QTL Analysis of Sheath Blight Resistance in Rice(Oryza sativa L.)

In this study, an indica variety Zhai Ye Qing 8 (ZYQ8) and a japonica variety Jing Xi 17(JX17) and their double haploid (DH) population were used. Sheath blight resistance of the DH population and their parents was investigated using a syringe inoculation method. Quantitative trait loci (QTL) analysis was undertaken based on a constructed molecular linkage map of this population in Hangzhou and Hainandao respectively. A total of four sheath blight resistant QTLs ( qSBR 2, qSBR 3, qSBR 7 and qSBR 11) were identified, and located on chromosome 2, 3, 7 and 11, respectively. Resistant alleles of qSBR 2, qSBR 3, and qSBR 7 were derived from the resistant parent ZYQ8, whereas resistant allele of qSBR 11 from susceptible parent JX17. qSBR 2, qSBR 3 and qSBR 7 were detected both in Hangzhou and Hainandao, whereas qSBR 11 was detected only in Hangzhou. Sheath blight response was significantly correlated with culm length and heading date in Hangzhou's test. Among the QTLs for culm length and heading date, only qCL 3 for culm length, was in the same chromosome region of qSBR 3 , the other QTLs were not linked to the QTLs for sheath blight resistance.