猕猴、白眉长臂猿、人类染色体普通型脆性部位和G-带的比较研究

以BrdU、FdU、MTX诱导猕猴、白眉长臂猿和人类染色体普通型脆性部位的表达,并对染色体脆性部位和染色体进化的关系以及三种灵长类染色体的同源性进行了比较分析。结果表明,近缘动物染色体同源区内的脆性部位在进化上是保守的,可作为染色体具有共同起源的标志,结合G-带的比较,可以用以阐明近缘动物染色体的同源性和染色体进化。

Comparison of the Common Fragile Sites and G-banding Patterns of Chromosomes among Rhesus Monkeys, White-browed Gibbons and Human Being

The peripheral lymphocyte cultures from Rhesus monkeys (Macaco mulatto),White-browed gibbons (Hylobates hoolock.) and human being were treated with BrdU (bromodeoxyuridine),FrdU (fluorodeoxyuridine) and MTX (methotrexate).A number of 22 Common fragile sites for Rhesus monkeys and 20 for white-browed gibbons were found.Chromosome homology and the relationship between fragile sites and chromosomal evolution were discussed.Several common fragile sites of human chromosomes have been found at the homologous chromosomal regions in Rhesus monkeys as well as in White-browed gibbons.Furthermore,the fragile sites at 1qH,1p32 of Rbesus monkey chromosomes may correspond to the homologous fragile sites at 1p21,1q31 of White-browed gibbons.All of these fragile sites at the homologous regions of chromosomes are evolutionarily conserved and may be the indicators of common origin of chromosomes as genetic markers.Combined w ith G-banding patterns of chromosomes,the conservative fragile sites at homologous regions of chromosomes may be useful for the study of chromosome homology and evolution in closely-related animals.Out results show that the differences of chromosome 1 between Rhesus monkeys and human may involve a pericentric inversion and a paracentric inversion.However,the differences of chromosome 1 between Rhesus monkeys and White-browed gibbons may conrean a pericentric inversion,a peracentric inverson,and a delection or a translocation.The main differences of chromosome 3 between Rhesus monkeys and human may involve a pericentric inversion.Out results indicate that the differences of chromosome 7 petween White-biowed gibbons and human may involve a paracentric inversion.Also,our results show that chromosome 7 of White-browed gibbons or human may represent the common ancestral chromosome of the higher primates (Hominoidea).