以Cytb基因部分序列分析大额牛系统地位及其种群现状

对12头大额牛Cytb基因部分序列447bp进行分析,共发现33个变异位点,定义了4种单倍型;结合GenBank中牛属普通牛、瘤牛、牦牛和印度褐牛4个近缘种的Cytb基因同源区序列进行分析,以水牛作为外群,分别采用邻接(NJ)法和最大简约(MP)法构建分子系统发育树,得到基本一致的拓扑结构,结果表明大额牛是独立于普通牛、瘤牛和印度褐牛之外的Bos属的一个独立的种。同时还指出目前我国有相当比例的大额牛受到外来物种的入侵,我国的大额牛保护工作正面临非常严峻的考验。

Phylogenetic Relationships and Status Quo of Colonies for Gayal Based on Analysis of Cytochrome b Gene Partial Sequences

Thirty-three mutations and four different haplotypes were found when cytochrome b(Cytb) gene partial sequences of 12 gayals were analyzed. Together with sequences of Bos indicus,Bos taurus,Bos grunniens,and Bos gaurus with Bubalus bubalis as the out group,the partial sequences of Cytb gene of gayals were aligned and base composition and nucleotide variation of Cytb gene were analyzed. The phylogenetic trees were constructed by the NJ method and the MP method respectively,both supporting almost the same topology. Gayal is an independent species of Bos from Bos indicus,Bos taurus,and Bos gaurus. The results also indicate that a great proportion of gayal bloodline was invaded by other species,and the protection of gayal is facing a formidable situation.