小鼠脊髓重与颈膨大截面积QTL研究

应用近交系小鼠A/J和C5 7BL/ 6J以及其F2 代重组小鼠定位脊髓重与颈膨大截面积数量性状基因座(QuantitativeTraitLoci,QTL)。结果表明 13个QTL与脊髓重和截面积相关 ,这些QTL分别位于 2、4、8、14、15、17、18、19和X染色体上。其中 6个QTL与脊髓重相关 ;4个QTL与颈膨大截面积相关 ;3个QTL与二者均相关。在 13个QTL中 ,3个QTL (P <0 0 1)———SC1(位于D15Mit15 8附近 )、SC2 (DXMit14 0附近 )和SC3(DXMit6 4附近 )其表型变异的解释率分别为 2 4 %、19%和 15 % ;加性效应分别为 - 3 78、3 4 1和 2 0 6mg。其他QTL的P值在 0 0 1～ 0 0 5之间。在上述 3个QTL中 ,SC1是唯一与脊髓重和面积均相关的QTL

Identification of QTLs for Weight and Cross-sectional Area on Cervical Enlargement of Spinal Cord in Mice

Two inbred strains of mice,A/J,C57BL/6J and F 2 intercross progenies,were used for QTL mapping for weight and cross-sectional area on cervical enlargement of spinal cord in mice. 13 QTLs located on Chromosome 2,4,8,14,15,17,18,19 and X,respectively,for these two traits were found. Six QTLs were responsible for the cord weight,four for the cross-sectional area and three for both. Among 13 QTLs,three QTLs (P<0.01) termed SC1 (located near D15Mit158),SC2 (DXMit140) and SC3 (DXMit64) accounted for 24%,19% and 15% of the total variance in weight phenotype,and -3.78,3.41 and 2.06 mg additive effect,respectively. The P value of other QTLs is between 0.01 and 0.05. SC1 is only one QTL that responsible for both weight and cross-sectional area in three QTLs above. This study revealed the location of major QTLs related size of spinal cord in mice,and may be helpful in fine mapping and ultimate identification of candidate genes.