水稻第六染色体长臂亚端粒区遗传图与物理图的整合

生物染色体亚闰区域在物种翰经过程中是高度活跃的。为了认识水稻染色体亚端粒区域的组织结构，用水稻第六染色体长臂亚端料区的ＲＦＬＰ标记Ｇ３４２和Ｒ１１６７作探针筛选ＢＡＣ文库，以得到的阳笥ＢＡＣ克隆为起点进行染色体眇地，构建了覆盖这２个分子标记区约５００ｋｂ的ＢＡＣ跨叠克隆群，将这一区域的跗图和物理图进行了整合。对１４个ＢＡＣ克隆插入末端进行了亚克隆，鉴定的７个亚克隆 端为单考贝或抵考贝序列，其中５个

Integration of the Genetic Map and the Physical Map of the Subterminal Region on the Longer Arm of Rice Chromosome 6

The region between RFLP markers G342 and R1167 was the subterminal part of the longer arm on the rice chromosome 6, because Shen et al. (1998) mapped two telomeric repeat associated sequences distal to G342. In order to integrate the genetic map and the physical map of the region, G342 and R1167 were firstly used to screen BAC library. Based on the positive clones detected by the two markers and chromosome walking by using the outer most insert-end of the overlapping clones, a contig containing 16 BAC clones which spanned 500kb was constructed. All the insert-ends of the BAC clones could be amplified with thermal asymmetric interlaced PCR. Fourteen insert-ends were subcloned. Seven of them were identified as a single or low copy sequences and five were mapped on the expected sites flanking G342 or R1167. The insert fragment isolated from the minimun tile BAC clones of the contig was used to screen a cDNA library and four different positive clones were detected.