广西十个不同产地的两面针中活性成分的分析

采用HPLC法对广西十个不同产地的两面针中具有抗肿瘤活性的氯化两面针碱和具有镇痛活性的 L-芝麻脂素的含量进行了分析和比较。发现在不同产地的两面针中氯化两面针碱和L-芝麻脂素的含量差别 都较大,广西百色的两面针中氯化两面针碱含量和L-芝麻脂素的含量均最高,氯化两面针碱0．467％,L-芝麻 脂素0．160％;广西金秀的两面针中氯化两面针碱含量和L-芝麻脂素的含量均最低,氯化两面针碱0．0490％, L-芝麻脂素0．0370％。分析的色谱柱为HYPERSIL BDS C18,测定氯化两面针碱的条件为,流动相乙腈: 0．02 mol／L KH2PO4溶液(34:66),流速为1．0 mL／min,柱温40℃,检测波长329 nm,进样量为20μL;测定 L-芝麻脂素的条件为,流动相乙腈:水(50:50),流速为1．0 mL／min,柱温40℃,检测波长287 nm,进样量为 20μL。该研究为两面针在抗肿瘤药物和镇痛等方面的开发利用提供了可靠的理论依据,具有重要应用价值。

The determination of nitidine chloride and L-sesamin of Zanthoxylum nitidum (Roxb.) DC growing in ten areas of Guangxi

Active compounds of Zanthoxylum nitidum growing in ten different areas of Guangxi were analysed in this study. The results were as follows:The content of nitidine chloride and L-sesamin had great difference in the ten growing areas of Guangxi. The highest content of the nitidine chloride 0. 467% and L-sesamin 0. 160% of Zanthoxylum nitidum was growing at Baise of Guangxi; the lowest content of the nitidine chloride 0. 0490% and L-sesamin 0. 0370% of Zanthoxylum nitidum was growing at Jinxiu of Guangxi. The high performance liquid chromatography (HPLC) was utilized for this purpose with the column of HYPERSIL BDS C18. For nitidine chloride, the mobile phase containing acetonitrile and 0. 02 mol/L monobasic potassium phosphate (34 : 66) ,the detection wavelength was 329 nm,flowing rate was 1. 0 mL/min,column temperature was 40 ℃,sample injection was 20 μL. For L-sesamin, the mobile phase containing acetonitrile and water (50 : 50) ,the detection wavelength was 287 nm,flowing rate was 1. 0 mL/min,column temperature was 40 ℃ ,sam- ple injection was 20 μL. This study will supply a reliable theoretic direction to develop and make use of Zan-thoxylum nitidum in anti-tumor,antinociceptive and so on.