| 吉粳88高效再生体系的建立 |
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| 引用本文： | 张春艳,王 义,孙春玉,王康宇,张美萍.吉粳88高效再生体系的建立[J].广西植物,2013(6):865-869. |
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| 作者姓名： | 张春艳 王 义 孙春玉 王康宇 张美萍 |
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| 作者单位： | 吉林农业大学, 吉林 长春 130118 |
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| 摘 要： | 以吉粳88为材料,通过对不同培养基和激素的筛选,确定最佳的再生体系。结果表明:NMB培养基为吉粳88最适的愈伤组织诱导培养基。通过L9(33)正交实验研究最适激素浓度配比,筛选出吉粳88愈伤组织诱导的最佳激素配比为2.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.10 mg/L NAA。在添加2.0 mg/L 6-BA+0.5 mg/L NAA的NMB基本培养基条件下,吉粳88的分化率最高,成培苗率也最高。
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| 关 键 词： | 吉粳88 培养基 诱导率 分化率 成苗率 |
Establishment of an efficient regeneration
system of Jijing88 |
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| ZHANG Chun-Yan,WANG Yi,SUN Chun-Yu,WANG Kang-Yu,ZHANG Mei-Ping.Establishment of an efficient regeneration
system of Jijing88[J].Guihaia,2013(6):865-869. |
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| Authors: | ZHANG Chun-Yan WANG Yi SUN Chun-Yu WANG Kang-Yu ZHANG Mei-Ping |
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| Institution: | Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | Jijing 88 was used as the research material and different media and hormone combinations were selected,in order to obtain its optimal in-vitro regeneration system. The results showed that NMB medium was the most suitable medium for rice callus induction among the 5 medium. The most suitable plant hormone ratio was investigated by using orthogonal test L9(33),and the induction rate was the highest when 2.0 mg/L 2,4-D,0.2 mg/L 6-BA,0.10 mg/L NAA were added. The differentiation rate and regeneration rate were the highest when 2.0 mg/L6-BA,0.5 mg/L NAA were added. |
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| Keywords: | Jijing 88 culture medium induction rate differentiation rate regeneration rate |
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