微量元素营养对桉树无性系苗木叶绿素及荧光参数影响

研究了不同微量元素营养液,对栽培的三种桉树无性系苗木叶绿素及荧光参数变化。结果表明:各无性系苗木在全部测试性状的差异表现出极显著。在六个测试的微量元素因子中,Fe(B)显著地影响苗木的Fo、Fm、Fv/Fo、Fv/Fm,Zn(C)显著地影响Fo、Fv/Fm,Mn(D)显著地影响叶绿素含量(SPAD值),Mo(F)显著地影响叶绿素含量、Fo,B(G)显著地影响叶绿素含量Fo、Fv/Fo、Fv/Fm。相关分析表明Fm与Fo、Fv/Fo与Fo、叶绿素含量与Fo、Fm、Fv/Fm与Fv/Fo均存在显著的相关性。随着微量元素Fe、Zn、Cu、Mo、B浓度增大,Fo、Fv/Fm均呈增大趋势,但在不利的高浓度环境下,Fv/Fm降低。对三个无性系叶绿素荧光比较,U₆与DH_32-29具有高的Fo、Fm和低的Fv/Fm、Fv/Fo,DH_201-2却具有低的Fo、Fm和高的Fv/Fm、Fv/Fo。对微量元素浓度变化,DH_32-29较敏感。因此,叶绿素荧光参数可作为诊断植物微量元素营养状况的指标之一。

Effects of trace elements on chlorophyll fluorescence parameters of clonal eucalypt seedling

The effects of level and form of trace elements on chlorophyll and its fluorescence in seedling leaves from 3 eucalypt clones were studied by way of sand culture. The results showed that the chlorophyll content and fluorescence parameters were influenced by factors significantly. The eucalypt clone was an important effective factor and had high significantly influence on all traits. In the factors of trace elements,there existed high significant differences in Fe(B)to Fo,Fm,Fv/Fo and Fv/Fm,Zn(C)to Fo and Fv/Fm,Mn(D)to chlorophyll content(SPAD value),Mo(F)to chlorophyll content and Fo,B(G)to chlorophyll content,Fo,Fv/Fo and Fv/Fm,respectively. There were also significant relationships between Fm and Fo,between chlorophyll content and Fo,Fm,Fv/Fm and Fv/Fo.With the increase of Fe,Zn,Cu,Mo,B solution nutrition,Fo and Fv/Fm increased,but Fv/Fm decreased under the higher concentration. Compared with chlorophyll fluorescence,U₆ and DH_32-29 had higher Fo,Fm value and lower Fv/Fm,Fv/Fo value,but DH_32-29was more susceptible to abundance and deficiency of trace elements in soil. The results also showed that applying chlorophyll fluorescence would be a good method to evaluate the health of eucalypt species in trace element nutrient.