玉米ZmNPR1基因的克隆及表达分析

该研究从玉米高抗自交系D863F中克隆了ZmNPR1基因(GenBank登录号为MH619241)的gDNA和cDNA序列，开放阅读框长1 866 bp，编码621个氨基酸，相对分子质量67.61 kD，等电点为5.46。系统进化树比对表明，玉米ZmNPR1蛋白和高粱SbNPR1蛋白的亲缘关系较近，相似性高达97%。实时荧光定量PCR结果表明，ZmNPR1在叶片中能够被水稻黑条矮缩病毒诱导并显著上调表达。同时ZmNPR1在玉米叶片、茎、根、雄穗、雌穗以及花丝中均有表达，在雌穗和叶片中的表达量较高。研究表明,ZmNPR1可能在玉米粗缩病抗病过程中起着重要作用。

Cloning and Expression Analysis of Maize ZmNPR1 Gene

The NPR1, a disease resistant regulator, plays a key role in plant systemic acquired resistance. In our study, we cloned the gDNA and cDNA sequence of ZmNPR1 (GenBank accession number is MH619241) from a maize resistant inbred line D863F. It was found to contain a 1 866 bp open reading frame(ORF), which may encode a 621 amino acid polypeptide with a protein molecular weight of about 67.61 kD and a theoretical isoelectric point of 5.46. Phylogenetic analysis showed that ZmNPR1 was closely related to SbNPR1 of sorghum with 97% similarity. Real time quantitative PCR analysis showed that ZmNPR1 expression could be induced by rice black streaked dwarf virus (RBSDV) and significantly up regulated in leaves. Meanwhile, ZmNPR1 was expressed in leaves, stems, roots, tassels, ears and silks, and highly expressed in ears and leaves. The results indicated that ZmNPR1 may play an important role in the maize rough dwarf disease (MRDD) resistance.