双色蜡蘑对黑松幼苗生长量及其根系形态的影响

为研究双色蜡蘑(Laccaria bicolor)对黑松(Pinus thunbergii)幼苗生长及其根系形态的影响,在营养杯培育条件下,用双色蜡蘑液体菌剂对黑松幼苗进行接种处理,接种第15、30、60、90、120天时取样,比较接种和未接种黑松幼苗的生物量、根系形态及根系分形维数的差异。结果表明:双色蜡蘑在黑松幼苗地上植株、地下根系的生长及其生物量的积累方面都表现出明显的促进作用。接种双色蜡蘑也显著改善了根系总长度、分支数、表面积、体积等参数和根系分形维数,并对地下根系生长的促进作用时间早于地上部分,且效应显著高于地上部分。接种双色蜡蘑第15~30天时对地上部分基本无影响,但对根系促进作用明显,而地上部分在第60天时开始表现出显著的生长效应。研究发现,双色蜡蘑能够成功定殖于黑松根部,促进黑松幼苗生长及其生物量的积累,同时显著促进根系总长度、分支数、表面积和体积增加,并使根系分形维数增大,表现出明显的促生作用,且对根系发育的显著促进作用早于地上部分。

Effect of Laccaria bicolor on Growth and Root Morphology of Pinus thunbergii Seedlings

In order to study the effect of Laccaria bicolor on the growth and root morphology of Pinus thunbergii seedlings, we inoculated the seedlings of P. thunbergii with liquid fungus of L. bicolor in nutrition cup. We compared the differences of biomass, root morphology and root fractal dimension between inoculated and uninoculated P. thunbergii seedlings. The results showed that the L. bicolor showed obvious promotion effect on growth and biomass accumulation of underground roots and aboveground plants of P. thunbergii seedlings. Inoculation of L. bicolor also significantly improved the total root length, forks, root surface area, root volume and root fractal dimension. Moreover, its promoting effect on the growth of underground roots was earlier than that of the aboveground part, and the effect was significantly higher than that of the aboveground part. 15-30 days after the inoculation, L. bicolor had no effect on the aboveground part, but it had obvious promotion effect on the root system. However, the aboveground part began to show significant growth response on the 60th day. In summary, the L. bicolor can colonize the root of P. thunbergii successfully, promote the growth of seedlings and the accumulation of biomass, increase the total length, forks, surface area and volume of root system, and increase the fractal dimension of root system. It showed obvious promoting effect on root development, and the promoting effect was earlier befor the aboveground part growth.