| 巴东木莲种子贮藏和萌发过程中同工酶分析 |
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| 引用本文： | 张德春,张博,刘曦,王希群,陈发菊.巴东木莲种子贮藏和萌发过程中同工酶分析[J].西北植物学报,2011,31(7):1453-1457. |
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| 作者姓名： | 张德春 张博 刘曦 王希群 陈发菊 |
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| 作者单位： | 1. 三峡大学,生物技术研究中心,湖北宜昌,443002
2. 国家林业局林产工业规划设计院,北京,100010 |
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| 基金项目： | 国家自然科学基金(30670202); 国家林业局林业科学技术项目(2008-03) |
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| 摘 要： | 以贮藏和萌发过程中的巴东木莲种子为材料,采用非变性聚丙烯凝胶电泳技术分析其种子中淀粉酶(AMY)、酯酶(EST)、超氧化物歧化酶(SOD)、过氧化物酶(POD)同工酶酶谱,并测定其酸性磷酸酶(ACP)和POD的活性,以探讨巴东木莲种子休眠和萌发过程中的生理生化变化特征.结果表明:巴东木莲种子在贮藏和萌发过程中,EST和SOD同工酶在萌发过程中表达增强,并不断有新酶的合成;AMY同工酶在萌发初期表达强度高且酶带数较多,到后期表达水平较低,其可能启动并控制种子萌发快慢;POD同工酶在萌发后期酶的活性增强,且酶的种类也增加,与EST和AMY同工酶的变化相适应.巴东木莲种子ACP和POD活性在储藏条件下以干藏种子最低,在萌发过程中总体上随发育进程呈升高的趋势,与同工酶电泳的结果吻合.因此,EST、AMY、SOD和POD同工酶酶谱变化及表达强弱可作为巴东木莲种子萌发各阶段转变的重要标志.
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| 关 键 词： | 巴东木莲 种子 同工酶 非变性聚丙烯凝胶电泳 |
Seed Isozyme Analysis of Manglietia patungensisduring Storage and Germination |
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| ZHANG De-chun,ZHANG Bo,LIU Xi,WANG Xi-qun,CHEN Fa-ju.Seed Isozyme Analysis of Manglietia patungensisduring Storage and Germination[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(7):1453-1457. |
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| Authors: | ZHANG De-chun ZHANG Bo LIU Xi WANG Xi-qun CHEN Fa-ju |
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| Institution: | ZHANG De-chun1,ZHANG Bo1,LIU Xi1,WANG Xi-qun1,2,CHEN Fa-ju1(1 Biotechnology Research Center,China Three Gorges University,Yichang,Hubei 443002,China,2 Planning and Design Academy of Forest Products Industry,State Forestry Administration,Beijing 100010,China) |
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| Abstract: | Amylase(AMY),esterase(EST),superoxide dismutase(SOD) and peroxidase(POD) isozymes were analyzed by non-denaturing polyacrylamide gel electrophoresis and the activities of ACP and POD were determined from the process of Manglietia patungensis seed store and germination.The results showed:the isozyme activities all had different degrees of changes in their corresponding control metabolisms.Among them,EST and SOD,with the passage of time,enhanced their expressions and the seed continually had some new enzyme(E... |
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| Keywords: | Manglietia patungensis seed isozyme PAGE |
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