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1.
外源多胺对莴苣种子萌发诱导及其与一氧化氮的关系   总被引:1,自引:0,他引:1  
以正常莴苣'挂丝红'(Lactuca sativa)种子为材料,采用外源多胺(Put、Spd、Spm)、硝普钠(SNP)及NO清除剂cPTIO处理,以DAQ作为NO荧光检测探针,研究多胺和NO在莴苣种子萌发过程中的相互关系.结果显示:(1)外源多胺尤其是亚精胺(Spd)对莴苣种子都有一定的促早萌作用并以0.5 mmol·L-1处理最佳,其效果在种子吸胀后前48 h最为显著;1.0 mmol·L-1亚精胺合酶抑制剂环己胺(CHA)对莴苣种子萌发有较强的抑制作用;(2)外源NO供体SNP对莴苣种子早萌有显著的促进作用并表现出浓度依赖性,且48 h后促进作用消失,与外源Spd促进种子早萌作用相似;(3)在外源Spd和SNP处理的同时,添加NO清除剂cPTIO可显著降低SNP和Spd对莴苣种子萌发的促进作用;在Spd和SNP处理后的种子近胚区均有较强的NO荧光产生,而cPTIO可猝灭Spd和SNP处理种子胚荧光的增强,并伴随着对Spd和SNP促进种子早萌作用的抑制.研究表明,多胺尤其是亚精胺在促进莴苣种子早萌过程中可能与NO的诱导产生有关.  相似文献   
2.
Differential seed dispersal, in which selfed and outcrossed seeds possess different dispersal propensities, represents a potentially important individual‐level association. A variety of traits can mediate differential seed dispersal, including inflorescence and seed size variation. However, how natural selection shapes such associations is poorly known. Here, we developed theoretical models for the evolution of mating system and differential seed dispersal in metapopulations, incorporating heterogeneous pollination, dispersal cost, cost of outcrossing and environment‐dependent inbreeding depression. We considered three models. In the ‘fixed dispersal model’, only selfing rate is allowed to evolve. In the ‘fixed selfing model’, in which selfing is fixed but differential seed dispersal can evolve, we showed that natural selection favours a higher, equal or lower dispersal rate for selfed seeds to that for outcrossed seeds. However, in the ‘joint evolution model’, in which selfing and dispersal can evolve together, evolution necessarily leads to higher or equal dispersal rate for selfed seeds compared to that for outcrossed. Further comparison revealed that outcrossed seed dispersal is selected against by the evolution of mixed mating or selfing, whereas the evolution of selfed seed dispersal undergoes independent processes. We discuss the adaptive significance and constraints for mating system/dispersal association.  相似文献   
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4.
香附子提取物B处理作物种子效应初报张建新(陕西省农业科学院农业测试中心,杨陵712100)赵伯善(西北农业大学农业化学系,杨陵712100)EffectofseedtreatmentwithextractBfromCyperusrotundus¥Zh...  相似文献   
5.
This paper reports on a study of mitochondrial activity in etiolated shoots of freshly harvested and moderately aged kernels of maize. Activity was investigated after incubation at a favourable temperature (25°C), sub-optimal temperature (13°C) and after a heat shock (46°C for 2h). Although impaired mitochondrial activity in shoots from moderately aged maize kernels was not detected at 25°C, deficiencies became evident under low temperature stress (13°C). State 3 oxygen uptake, cyanide-insensitive oxygen uptake and cytochrome oxidase activity were lower in mitochondria from these shoots at 13°C than in mitochondria from shoots of freshly harvested kernels at this temperature. After a heat shock, cyanide-insensitive oxygen uptake was higher, and cytochrome oxidase activity lower, in shoots of aged kernels than in shoots of fresh kernels. No significant differences in ADP: O ratio or succinate dehydrogenase activity occurred between mitochondria from shoots of the two seed lots in any of the temperature treatments.  相似文献   
6.
Invasion of alien plant species (IAS) represents a serious environmental problem, particularly in Europe, where it mainly pertains to urban areas. Seed germination traits contribute to clarification of invasion dynamics. The objective of this research was to analyze how different light conditions (i.e., 12-hr light/12-hr darkness and continuous darkness) and temperature regimes (i.e., 15/6°C, 20/10°C and 30/20°C) trigger seed germination of Ailanthus altissima (AA), Phytolacca americana (PA) and Robinia pseudoacacia (RP). The relationship between seed germination and seed morphometric traits was also analyzed. Our findings highlight that temperature rather than light was the main environmental factor affecting germination. RP germinated at all tested temperatures, whereas at 15/6°C seeds of AA and PA showed physiological dormancy. RP had a higher germination capacity at a lower temperature, unlike AA and PA, which performed better at the highest temperatures. Light had a minor role in seed germination of the three species. Light promoted germination only for seeds of PA, and final germination percentage was 1.5-fold higher in light than in continuous darkness. Seed morphometric traits (thickness [T], area [A] and volume [V]) had a significant role in explaining germination trait variations. The results highlight the importance of increasing our knowledge on seed germination requirements to predict future invasiveness trends. The increase in global temperature could further advantage AA and PA in terms of germinated seeds, as well as RP by enhancing the germination velocity, therefore compensating for a lower germination percentage of this species at the highest temperatures.  相似文献   
7.
对中甸刺玫Rosa praelucens种子结构及其透水性,赤霉素处理对胚的影响,果壳、种皮、胚乳的粗提物活性进行研究。结果表明:(1) 中甸刺玫外种皮是由多层排列紧密的厚壁细胞组成,内种皮为坚硬致密的栅栏组织;(2) 种皮对种子的吸胀阻碍较大, 未处理的种子吸水率较低,吸水13 d后增加量为18.82%;(3) 果实结籽率为0.69%,多数果实中没有饱满的种子;(4) 赤霉素100 mg·kg-1预处理种子可加快胚的萌发速率;(5) 胚乳、种皮、果壳中存在内源抑制物。中甸刺玫种子的休眠是由其形态和生理特点引起的综合休眠。  相似文献   
8.
于晓  严成  魏岩 《生态学报》2009,29(3):1616-1621
盐生草的果实和种子存在二型性,这两种类型的种子在形状、大小、颜色及包被其花被片背部是否具翅上均有显著差异.绿色种子,圆形,直径为(1.552±0.116) mm,宿存花被革质,背部有紫红色翅状附属物,单粒重为(0.808±0.033)mg;黄色种子,椭圆形,长为(1.752±0.155) mm,宽为(1.146±0.088) mm,宿存花被革质,背部无翅状附属物,单粒重为(0.568±0.011) mg.两种种子在3个变温条件(5/25℃、5/25℃、15/25℃,暗12 h /光12 h)下的萌发率均较低,绿色种子为36%,而黄色种子为17%(15℃/25℃).延长储藏时间和划破种皮均能显著提高绿色种子的萌发率,表明绿色种子属于非深度生理休眠.随着储藏时间的延长,黄色种子的萌发率也能缓慢提高,但不显著,而划破种皮能够显著促进其萌发,表明黄色种子属于深度生理休眠.  相似文献   
9.
We compared various aspects of the seed biology of eight non-pioneer tree species from a tropical seasonal rain forest in Xishuangbanna, SW China, that differ in time of dispersal, size and fresh seed moisture content (MC). Seeds were tested for germination under laboratory conditions after dehydration to different moisture levels and under 3.5, 10 and 30% solar irradiances in neutral-shade houses. For six species, germination was also compared in forest understory (3.5% light) and center of a forest gap (32.5% light). Under continuous dehydration over activated silica gel, 100% of seeds of four species had lost the ability to germinate after 48 h, and those of all species except Castanopsis hystrix (decreased from >90 to 30% germination) had lost the ability to germinate after 120 h. Four species did not differ in final germination percentages at the three irradiances (i.e. uniform germination). However, final germination percentages of Horsfieldia pandurifolia and Litsea pierrei var. szemaois were significantly lower in 30% than in 10 or 3.5% light, and seeds of Antiaris toxicaria and C. hystrix germinated to higher percentages in 30 and 10% than in 3.5% light. Mean time to germination (MTG) of the eight species (forest and shade house data combined) ranged from 5–5 days for Pometia tomentosa to 72–207days for L. pierrei; MTG for four species was ≤21 days. There was no obvious relationship between relative desiccation resistance and either time of dispersal, MTG or uniformity of germination at the three light levels, or between seed size and MC or MTG. However, the relationship between seed MC at maturity (25–60% fresh mass basis) and MC at 50% loss of seed viability (12.4–42.5%) was significant. Seven of the species fit Garwood’s (Ecol Monogr 53:159–181, 1983) rapid-rainy germination syndrome and one, L. pierrei, either her delayed-rainy or intermediate-dry germination syndrome. However, fresh, non-dehydrated seeds of all eight species germinated in ≤30 days at constant 30°C in light.  相似文献   
10.
The founding heterochronic microRNAs, lin-4 and let-7, together with their validated targets and well-characterized phenotypes in C. elegans, offer an opportunity to test functionality of microRNAs in a developmental context. In this study, we defined sequence requirements at the microRNA level for these two microRNAs, evaluating lin-4 and let-7 mutant microRNAs for their ability to support temporal development under conditions where the wild-type lin-4 and let-7 gene products are absent. For lin-4, we found a strong requirement for seed sequences, with function drastically affected by several central mutations in the seed sequence, while rescue was retained by a set of mutations peripheral to the seed. let-7 rescuing activity was retained to a surprising degree by a variety of central seed mutations, while several non-seed mutant effects support potential noncanonical contributions to let-7 function. Taken together, this work illustrates both the functional partnership between seed and non-seed sequences in mediating C. elegans temporal development and a diversity among microRNA effectors in the contributions of seed and non-seed regions to activity.  相似文献   
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