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植物不同种属间共用微卫星引物的研究
引用本文:王丽,赵桂仿.植物不同种属间共用微卫星引物的研究[J].西北植物学报,2005,25(8):1540-1546.
作者姓名:王丽  赵桂仿
作者单位:西北大学,生命科学学院,秦岭生物多样性研究中心,西安,710069
基金项目:国家自然科学基金(39770087);陕两省自然科学基金(2001SM20)资助
摘    要:微卫星标记是近年来发展起来的建立在PCR基础卜的第二代分子标记,具有分布均匀、多态性高、共显性、选择中性等优点,已用于品种鉴定、构建连锁图谱、构建物理图谱、遗传多样性、居群和进化等方面的研究。由于来源于数据库的微卫星序列不能代表整个基因组微卫星的分布情况,而且数据库中的DNA序列仅局限于一些模式植物及经济作物。同时,通过基因组文库筛选获得微卫星序列既耗时又耗资。最近有很多研究表明,从某种植物来的微卫星引物可用于其近缘物种的扩增。本文概括介绍了植物不同种属间共用微卫星引物的研究情况及意义。

关 键 词:分子标记  微卫星  共用引物
文章编号:1000-4025(2005)08-1540-07
收稿时间:03 11 2004 12:00AM
修稿时间:2004-03-11

Micro-satellite Primers Shared by Different Plant Species and Genera
Wang Li;Zhao GuiFang.Micro-satellite Primers Shared by Different Plant Species and Genera[J].Acta Botanica Boreali-Occidentalia Sinica,2005,25(8):1540-1546.
Authors:Wang Li;Zhao GuiFang
Abstract:Micro-satellite markers are the second-generation molecular markers, which have been developed in recent years and are characterized by such favorable characteristics as even distribution, high polymorphism, co-dominance and selective neutrality, and used in the studies concerned with variety evaluation, linkage-map construction and physical-map construction, genetic diversity, population and evolution. Micro-satellite sequences from data banks cannot represent the micro-satellite distribution of a whole genome and DNA sequences from data bases only limited to some modeled plants and cash crops. Furthermore, it is time-consuming and fund-costing to screen micro-satellite sequences by means of genomic libraries. Many recent researches have denoted that the micro-satellite primers from a species can be used for the amplification for its genetically close species. The paper deals with the researches and importance of micro-satellite primers shared by different plant species and genera.
Keywords:molecular marker  micro-satellite  shared primers
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