| 花生LEC1基因的克隆及表达研究 |
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| 引用本文： | 李爱芹,夏晗,王兴军,李长生,赵传志,毕玉平,单雷,唐桂英.花生LEC1基因的克隆及表达研究[J].西北植物学报,2009,29(9). |
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| 作者姓名： | 李爱芹 夏晗 王兴军 李长生 赵传志 毕玉平 单雷 唐桂英 |
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| 作者单位： | 山东省农业科学院高新技术研究中心,山东省作物与畜禽品种改良生物技术重点实验室,农业部黄淮海作物遗传改良与生物技术重点开放实验室,济南,250100 |
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| 基金项目： | 国家863计划，山东省农业科学院创新基金项目 |
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| 摘 要： | 通过构建花生未成熟种子cDNA文库,结合大规模EST测序,从花生中克隆了LEC1基因2个成员的全长cDNA序列.序列分析表明,花生中LEC1的2个成员的序列在B结构域高度保守,属于LEC1型HAP3亚基.不同植物中LEC1基因在B结构域以外的序列差异很大.利用花生cDNA芯片和半定量RT-PCR对花生LEC1进行表达研究表明,LEC1在种子中高水平表达,在种子发育的不同时期表达有差异.
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| 关 键 词： | 花生 cDNA文库 表达分析 |
Cloning and Expression Analysis of Peanut(Arachis hypogaea L.) LEC1 |
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| LI Ai-qin,XIA Han,WANG Xing-jun,LI Chang-sheng,ZHAO Chuan-zhi,BI Yu-ping,SHAN Lei,TANG Gui-ying.Cloning and Expression Analysis of Peanut(Arachis hypogaea L.) LEC1[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(9). |
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| Authors: | LI Ai-qin XIA Han WANG Xing-jun LI Chang-sheng ZHAO Chuan-zhi BI Yu-ping SHAN Lei TANG Gui-ying |
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| Institution: | LI Ai-qin,XIA Han,WANG Xing-jun,LI Chang-sheng,ZHAO Chuan-zhi,BI Yu-ping,SHAN Lei,TANG Gui-ying(High-Tech Research Center,Sh,ong Academy of Agricultural Sciences,Key Laboratory for Genetic Improvement of Crop,Animal , Poultry of Sh,ong Province,Key Laboratory of Crop Genetic Improvement , Biotechnology,Huanghuaihai,Ministry of Agriculture,Ji'nan 250100,China) |
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| Abstract: | By constructing a cDNA library of peanut immature seed and large scale EST sequencing,we obtained 15 ESTs which were homologues of Arabidopsis leafy cotyledon 1(LEC1).Full length of two members of peanut LEC1(AhLEC1A and AhLEC1B) were cloned.The results show that B domain in the two members of AhLEC1 and LEC1 from other plant species is highly conserved,belonging to LEC1-type HAP3 subunits,whereas sequences of upstream and downstream of B domain regions are different significantly.Peanut cDNA microarray and... |
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| Keywords: | LEC1 |
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