水稻香味基因分子标记的开发及应用

根据香稻材料在Badh2基因(甜菜碱醛脱氢酶基因)第7外显子的突变位点,设计了分子标记YY5-YY8用来区分这种突变类型的香稻材料。同时结合已报道的针对Badh2在第2、4、5外显子处突变设计的功能标记InDel-E2和FMbadh2-E4-5,对80份不同生态区香稻材料进行了标记检测。结果表明,26份材料属于第7外显子突变类型,37份材料属于第2外显子突变类型,无第4、5外显子突变类型材料。该研究构建了针对Badh2突变的新的分子标记YY5-YY8,并且鉴定了上述香稻材料的突变类型,为优质香稻分子育种奠定基础。

Development of Molecular Markers for Fragrant Gene and Its Application

According to the mutation in exon 7 of Badh2 (Betaine aldehyde dehydrogenasegene) in fragrant rice,we designed a functional marker YY5-YY8,which can be used to check rice materials with this mutation or not.Other two reported markers for exon 2-mutated and exon 4 or 5-mutated types of Badh2,InDel-E2 and FMbadh2-E4-5,were also used to analyze the mutations of Badh2 in 80 fragrant rice materials from different ecological regions.It showed that 26 fragrant rice materials were of the exon 7-mutated type,37 fragrant rice materials were of exon 2-mutated type and none of them was found to be of exon 4 or 5-mutated type.This study constructed a new molecular marker YY5-YY8 for checking the mutation of Badh2 and identified the mutation types of these fragrant rice varieties for breeding high quality fragrant rice.