培养条件对新疆紫草毛状根生长及紫草素含量的影响

以发根农杆菌诱导的新疆紫草毛状根为试验材料,采用二阶段液体培养法,首次建立了新疆紫草毛状根培养技术体系。结果显示:采用SH无铵培养基、pH 5.8时有利于毛状根的生长。培养12d时毛状根的增殖倍数达最高,平均10.26倍;毛状根生产的继代周期为25～30d;4种树脂吸附的紫草素及其衍生物含量均较对照(不添加树脂)高,以NKA-9所吸附的紫草素及其衍生物含量最高,为2.38%,较对照提高0.97倍。培养10d时添加NKA-9树脂,紫草素及其衍生物含量平均为3.64%,是对照的3.08倍。研究表明,生长阶段采用液体培养可以使新疆紫草毛状根快速增殖,生产阶段添加大孔吸附树脂能够提高紫草素及其衍生物含量。

Influence of Culture Conditions on Arnebia euchroma Hairy Roots Growth and Shikonin Content

Arnebia euchroma(Royle) Johnst hairy roots induced by Agrobacterium rhizogenes as test materials,the culture systems of A.euchroma hairy roots were established by two-stage liquid method at the first time.The results showed that it was beneficial to the growth of the hairy roots under the conditions which hairy roots inoculated with SH(no NH4+) liquid medium and pH 5.8.Trained 12 days,hairy roots proliferation has increased as the highest multiples by an average of 10.26 times;Hairy roots growth cycle was 25 to 30 days;The results suggested that the total shikonin and its derivatives contents in 4 models of macroporous adsorption resin were all higher than that in CK;For NKA-9,the sadsorption shikonin and its derivatives contents was 2.38% as the most one that increased by 0.97 times than CK.The results indicated that adding NKA-9 resin after cultured 10 days,the average shikonin and its derivatives was 3.64% that increased by 3.08 times than CK.The conclusion of the experiment is that the liquid cultured can make A.euchroma hairy roots have the qualities of rapid growth rates by the growing stage,and adding macroporous adsorption resin can increase the content of shikonin and its derivatives by theproducing stage.