Arnebia euchroma (Royle) Johnst hairy roots induced by Agrobacterium rhizogenes as test materials,the culture systems of A.euchroma hairy roots were established by twostage liquid method at the first time.The results showed that it was beneficial to the growth of the hairy roots under the conditions which hairy roots inoculated with SH(no NH₄⁺) liquid medium and pH 5.8.Trained 12 days,hairy roots proliferation has increased as the highest multiples by an average of 10.26 times；Hairy roots growth cycle was 25 to 30 days;The results suggested that the total shikonin and its derivatives contents in 4 models of macroporous adsorption resin were all higher than that in CK；For NKA9,the sadsorption shikonin and its derivatives contents was 2.38% as the most one that increased by 0.97 times than CK.The results indicated that adding NKA9 resin after cultured 10 days,the average shikonin and its derivatives was 3.64% that increased by 3.08 times than CK.The conclusion of the experiment is that the liquid cultured can make A.euchroma hairy roots have the qualities of rapid growth rates by the growing stage,and adding macroporous adsorption resin can increase the content of shikonin and its derivatives by theproducing stage.