毛刺槐花药培养及再生植株的获得

以毛刺槐的花药为材料，开展其组织培养和植株再生系统的研究。结果显示：将毛刺槐的花药接种在MS附加2，4—D0．1mg／L和BA3．0mg／L的培养基上，20d时花药愈伤组织诱导率可达41．5％。花药愈伤组织在MS附加BA5．0mg／L的分化培养基上继代培养2个月后，可分化出许多绿色的芽点，待不定芽长至2—3cm高时将其切下，转入MS附加IBA1．0mg／L的生根培养基上，2周后即可得到完整的再生植株。同时，研究就4℃低温预处理和蔗糖浓度对毛刺槐花药培养的影响进行了研究和讨论。

Anther culture and plant regeneration of Robinia hispida

The anthers of Robinia hispida were cultured and the results showed that the highest induction rate of callus was 41. 5% when they were cultured on MS medium supplemented with 2,4-D 0. 1 mg/L and BA 3. 0 mg/L for 20 days. Green buds formed from the callus after 2 months subcultured on MS medium supplemented with BA 5. 0 mg/L. When the shoots grew to 2-3 cm long,they were detached and trans-ferred to MS medium supplemented with IBA 1. 0 mg/L for rooting. The shoots produced roots within 2 weeks of culture and formed complete plantlets. At the same time,the effects of pretreatment of low tem-perature at 4 C and the concentration of sucrose on anther culture were studied and discussed.