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| 影响苹果离体叶片早期转化效率因素的研究 | |
| 引用本文： | 秦玲,李嘉瑞,李明,韩礼星,黄贞光,赵改荣.影响苹果离体叶片早期转化效率因素的研究[J].西北植物学报,2004,24(1):25-30. |
| 作者姓名： | 秦玲 李嘉瑞 李明 韩礼星 黄贞光 赵改荣 |
| 作者单位： | 1. 中国农业科学院郑州果树研究所,郑州,450009;西北农林科技大学,园艺学院,陕西杨陵,712100 2. 西北农林科技大学,园艺学院,陕西杨陵,712100 3. 中国农业科学院郑州果树研究所,郑州,450009 |
| 基金项目： | 国家自然科学基金资助项目 39970522 |
| 摘 要： | 利用gus为报告基因，通过统计gus基因瞬时表达的GUS 位点数和形成的愈伤组织，快速检测外源基因是否导入植物细胞及转化效率，以及对苹果转化早期阶段的影响因素。结果表明，A281对苹果叶片的浸染率比LBA4404浸染率要高，转化效率要好；叶片近轴面接触培养基转化效率较好，显著高于远轴面；整叶划痕为最佳切割方式，有利于提高苹果叶片转化率；苹果基因型、菌液浓度和共培养天数对不同基因型苹果的转化效率影响很大。 |
| 关 键 词： | 苹果 遗传转化 GUS＋检测 转化效率 |
| 文章编号： | 1000-4025(2004)01-0025-06 |
Study on factors affecting transfer efficiency in apple leaves in vitro during early steps |
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| QIN Ling.Study on factors affecting transfer efficiency in apple leaves in vitro during early steps[J].Acta Botanica Boreali-Occidentalia Sinica,2004,24(1):25-30. | |
| Authors: | QIN Ling |
| Abstract: | The factors affecting transfers of gus gene to apple leaf in vitro were studied during early steps by counting the number of GUS~+ sites and calli developing on leaves,in order to detect quickly gus gene introduce and its transfer efficiency.The results showed that A281 for apple transformation was better than LBA4404;The adaxial side in contact with the medium was higher.The transformation of apple decided its genotype.The bacterial cell density and the period of cocultivation of the different genotype apple had significantly effect on transfer efficiency. |
| Keywords: | apple genetic transformation GUS assays transfer efficiency |
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