| 百合查尔酮合成酶(CHS)基因的克隆与分析 |
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| 引用本文： | 杨丽,刘雅莉,王跃进,徐伟荣.百合查尔酮合成酶(CHS)基因的克隆与分析[J].西北植物学报,2006,26(5):933-936. |
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| 作者姓名： | 杨丽 刘雅莉 王跃进 徐伟荣 |
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| 作者单位： | 西北农林科技大学,园艺学院,农业部西北园艺种质资源与遗传改良重点开放实验室,陕西,杨陵,712100 |
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| 摘 要： | 以东方百合“索邦”基因组DNA为模板进行PCR扩增,将得到的目的片段克隆至pGEM-T easy载体后进行测序.结果表明,目的序列全长1 307 bp,经BLA ST分析,与鸢尾、玉米、水稻等植物的查尔酮合成酶(CHS)基因核苷酸同源性在70%以上;与已经克隆的CHS cDNA序列相比,CHS DNA序列中包含2个外显子和1个内含子,内含子全长82 bp,符合TG-AG特征.将得到的序列提交G enB ank,序列号为DQ 471951.
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| 关 键 词： | 百合 CHS 基因克隆 序列分析 |
| 文章编号： | 1000-4025（2006）05-0933-04 |
| 收稿时间： | 2005-12-26 |
| 修稿时间： | 2005-12-262006-04-11 |
Cloning and Analysis of Chalcone Synthase Genes in Lilium |
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| YANG Li,LIU Ya-li,WANG Yue-jin,XU Wei-rong.Cloning and Analysis of Chalcone Synthase Genes in Lilium[J].Acta Botanica Boreali-Occidentalia Sinica,2006,26(5):933-936. |
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| Authors: | YANG Li LIU Ya-li WANG Yue-jin XU Wei-rong |
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| Institution: | College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry, Key Laboratory of Horticulture Plant Germplasm and Genetic Improvement of C hina Agricuh u re Minist ry, Yangling, Shaanxi 712100,China |
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| Abstract: | DNA genome of the Lilium hybrid Sorbonne was used as the template to conduct PCR amplification and a target fragment in the amplification was cloned to vector pGEM-T easy and then sequenced.The results indicated that the whole length of the fragment was 1 307 bp and founded by BLAST to be more than 70% as homologous as chalcone synthase of Iris hollandica,maize and rice in nucleotide.Compared with the sequences of cloned CHS cDNA,the sequence of CHS DNA contained two exons and one inron and the intron was 82 bp starting with TG and ending with AG.The sequence was reported to the GenBank and coded as DQ471951. |
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| Keywords: | Lilium CHS gene cloning sequencing |
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