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濒危物种山红树居群遗传结构的RAPD分析
引用本文:苏志龙,殷寿华,吴成军,程在全.濒危物种山红树居群遗传结构的RAPD分析[J].云南植物研究,2005,27(2):181-186.
作者姓名:苏志龙  殷寿华  吴成军  程在全
作者单位:1. 中国科学院西双版纳热带植物园,云南,勐仑,666303;中国科学院研究生院,北京,100039
2. 中国科学院西双版纳热带植物园,云南,勐仑,666303
3. 云南省农业科学院生物技术研究所,云南,昆明,650223
基金项目:中国科学院知识创新工程重要方向性项目KSCX2-SW-117
摘    要:利用RAPD分子标记检测了云南省3个山红树居群的遗传多样性和居群遗传结构。10个引物共检测到54个位点, 其中多态位点11个, 占20 37%。与其他的濒危物种相比, 山红树居群内的遗传多样性很低, 居群间的遗传分化很大(78 65%)。大量经济植物的种植加上人为的破坏, 使山红树的生境遭到严重破坏, 数量大为减少, 可能导致了山红树遗传多样性的丧失、居群间较高的遗传分化。基于以上结果, 探讨了山红树进一步的迁地保护措施。

关 键 词:山红树  遗传多样性  迁地保护  居群遗传结构  RAPD
文章编号:0253-2700(2005)02-0181-06
修稿时间:2004年8月14日

Analysis of Genetic Structure of the Endangered Species Pellacalyx yunnanensis (Rhizophoraceae) by RAPD
SU Zhi-Long,YIN Shou-Hua,WU Cheng-Jun,CHENG Zai-Quan.Analysis of Genetic Structure of the Endangered Species Pellacalyx yunnanensis (Rhizophoraceae) by RAPD[J].Acta Botanica Yunnanica,2005,27(2):181-186.
Authors:SU Zhi-Long  YIN Shou-Hua  WU Cheng-Jun  CHENG Zai-Quan
Institution:SU Zhi-Long~
Abstract:The genetic diversity and population genetic structure of three populations of Pellacalyx yunnanensis in Yunnan Province were estimated by RAPD markers.Fifty-four loci were amplified with 10 oligonucleotide primers,of which 11 loci (20.37%) were polymorphic.The genetic diversity within a population was very low,while the genetic differentiation very high (78.65%)among populations compared with other endangered species.The habitat of P.yunnanensis was severely destroyed by the people's activities and the economic plants planted,which may result in the loss of genetic diversity and high genetic differentiation.Implications for the further conservation strategies of this endangered species were discussed based on these results.
Keywords:Pellacalyx yunnanensis  Genetic diversity  Ex-situ conservation  Population genetic structure  RAPD
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