| 毛竹笋全长cDNA文库构建 |
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| 引用本文： | 刘志伟,张智俊,韩国民,何沙娥,杨丽.毛竹笋全长cDNA文库构建[J].生物技术通报,2010(2). |
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| 作者姓名： | 刘志伟 张智俊 韩国民 何沙娥 杨丽 |
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| 作者单位： | 浙江省现代森林培育技术重点实验室,临安,311300 |
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| 基金项目： | 国家"863"项目，浙江省自然科学基金资助项目，浙江省现代森林培育技术重点实验室开放基金 |
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| 摘 要： | Gateway技术构建cDNA文库,利用了λ噬菌体的位点特异性重组,避免使用限制性内酶切割cDNA,能够解决常规方法构建cDNA文库的技术缺陷。应用Gateway技术构建毛竹笋cDNA文库,经检测cDNA入门文库的滴度达到1.7×106cfu/mL,文库总容量为8.5×106cfu,平均插入片段在1.0 kb以上。毛竹笋文库的构建为进一步克隆毛竹纤维化分子机理打下了基础。
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| 关 键 词： | Gateway技术 毛竹笋 纤维素合成酶基因 cDNA入门文库 |
Construction of a cDNA Library of Phyllostachs pubescens Using Gateway Technology |
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| Liu Zhiwei,Zhang Zhijun,Han Guomin,He Shae,Yang Li.Construction of a cDNA Library of Phyllostachs pubescens Using Gateway Technology[J].Biotechnology Bulletin,2010(2). |
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| Authors: | Liu Zhiwei Zhang Zhijun Han Guomin He Shae Yang Li |
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| Institution: | Liu Zhiwei Zhang Zhijun Han Guomin He Shae Yang Li(Zhejiang Provincial Key Lab for Modern Silvicultural Techndogy,Linan 311300) |
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| Abstract: | A cDNA library was constructed based on the Gateway system that is a methed to construct a high quality cDNA library without the use of restriction enzyme for cloning.This is the first report that the Gateway system is used to construct a cDNA library for Phyllostachs pubescens.The entry cDNA library was constructed in this report has a high titer of 1.7 106 cfu/mLand contain a total clones of 8.5 106 cfu/mL,with an average inserts size of about 1.0 kb.The construction of the cDNA library of bamboo shoots(P... |
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| Keywords: | Gateway method Moso bamboo shoot Cesa gene cDNA entry library |
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