| 相关序列扩增多态性(SRAP)标记及其应用研究进展 |
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| 引用本文： | 唐玉海,郭春芳,张木清.相关序列扩增多态性(SRAP)标记及其应用研究进展[J].生物技术通报,2006(Z1):237-241. |
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| 作者姓名： | 唐玉海 郭春芳 张木清 |
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| 作者单位： | 1. 农业部甘蔗遗传改良与生理生态重点开放实验室,福州,350002
2. 农业部甘蔗遗传改良与生理生态重点开放实验室,福州,350002;福建教育学院,福州,350001 |
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| 基金项目： | 福建省自然科学基金项目(B0510025)，福建省教育厅科技计划项目(JA05333) |
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| 摘 要： | SRAP是一项基于PCR技术的分子标记技术,利用其独特的引物设计对基因组的开放阅读框(ORFs)进行特异扩增,利用个体以及物种的内含子、启动子和间隔序列的不同,产生基于内含子和外显子的SRAP多态性。阐述了SRAP的原理和流程,详细论述了SRAP标记目前在植物遗传多样性、作物品种鉴定、遗传图谱构建等方面的研究进展及应用前景。
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| 关 键 词： | SRAP 分子标记 PCR技术 |
Sequence-Related Amplified Polymorphism(SRAP) Markers and Applications |
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| Tang Yuhai,Guo Chunfang,Zhang Muqing.Sequence-Related Amplified Polymorphism(SRAP) Markers and Applications[J].Biotechnology Bulletin,2006(Z1):237-241. |
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| Authors: | Tang Yuhai Guo Chunfang Zhang Muqing |
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| Abstract: | SRAP is a molecular marker based on Polymerase Chain Reaction(PCR), it amplifies the open reading frames(ORFs) using the specific primers. Because of the differences of the introns, promoters and spacers among individuals, it can produce polymorphism based on introns and extrons .This paper summarized its principle and protocol, described its application in germplasm genetic diversity analysis, genetics mapping and identification of crop cultivars in detail. The prospect of the application of SRAP markers was discussed. |
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| Keywords: | SRAP Molecular marker Polymerase Chain Reaction(PCR) |
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