一株高产黑色素香灰菌菌株的鉴定、筛选及培养条件的优化

为筛选一株产黑色素能力强的菌株并优化其培养条件。通过ITS测序鉴定11株供试菌株，以菌丝生长速度、平板L值等指标筛选出一株产黑能力强的香灰菌，并对其生长所需碳源、氮源、pH等培养条件进行优化。研究表明，11株供试菌株均为香灰菌（Hypoxylon sp.），其中Hp.sp0006菌丝生长速度较快、菌球大且均匀、L值最低，并且发酵液黑色素含量最高。该菌株最优培养条件是，以葡萄糖为碳源、牛肉浸膏为氮源、碳氮比20∶1并添加10 mg维生素B₁，黑色素含量可达（1.21±0.17）g/L。香灰菌Hp.sp0006是一株产黑色素较高的菌株，优化后的培养基更有利于黑色素的合成，为香灰菌黑色素的开发利用奠定基础。

Screening,Identification, and Optimization of Culture Conditions of a Melanin High-yielding Strain of Hypoxylon sp.

In order to screen a strain with strong melanin-producing ability and optimize its culture conditions, 11 tested strains were identified by ITS sequencing, and a ash-producing fungus with strong black-producing ability was screened based on mycelial growth rate, plate L value and other indicators, and the carbon source, nitrogen source, pH and other culture conditions required for its growth were optimized. The study showed that all 11 test strains were Hypoxylon sp. Among them, Hp.sp0006 had faster mycelial growth, larger and more uniform balls, the lowest L value, and the highest melanin content in the fermentation broth. The optimal culture conditions for this strain were glucose as the carbon source, beef extract as the nitrogen source, a 20∶1 carbon to nitrogen ratio and the addition of 10 mg vitamin B₁, with a high yield of melanin up to（1.21±0.17）g/L melanin. Hp.sp0006 is a strain producing higher melanin. The optimized medium is more conducive to the synthesis of melanin, which lays the foundation for the development and utilization of melanin in Hypoxylon sp.