| 野生黄花苜蓿冷诱导基因克隆及序列分析 |
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| 引用本文： | 刘亚玲,王俊杰,云锦凤,赵彦,石凤敏.野生黄花苜蓿冷诱导基因克隆及序列分析[J].生物技术通报,2010(4). |
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| 作者姓名： | 刘亚玲 王俊杰 云锦凤 赵彦 石凤敏 |
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| 作者单位： | 1. 内蒙古农业大学生态环境学院,呼和浩特,010019;内蒙古草品种育繁工程技术中心,呼和浩特,010019
2. 内蒙古农业大学生态环境学院,呼和浩特,010019;内蒙古农牧业科学院,呼和浩特,010031 |
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| 基金项目： | 国家科技支撑项目，国家自然科学基金项目，内蒙古农业大学博士科研启动基金项目 |
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| 摘 要： | 根据紫花苜蓿抗寒基因cas15B(登录号:L12462)的cDNA序列,设计1对特异引物,以经过低温胁迫的黄花苜蓿总RNA为模板,采用RT-PCR方法克隆获得了550bp的cDNA片段。测序和序列分析结果表明,扩增片段长度为550bp,编码159个氨基酸。主要由Gly(甘氨酸)、Glu(谷氨酸)、His(组氨酸)、Lys(赖氨酸)这4种氨基酸组成,占总量的70%。含1个重复了5次的10肽基序,其序列为Lys-Gly-Glu-Gln-His-Gly-His(Phe)-Val(Leu)-Gly-Gly。经序列比较分析,该片段与紫花苜蓿冷诱导基因CAS15B的核苷酸、氨基酸的同源性均为90%,命名为MfCAS15-1。亚细胞结构定位分析结果显示,MfCAS15-1是一种定向到核的蛋白,在调节或维持核的结构与功能方面起作用。本研究在黄花苜蓿中成功获得了抗寒基因同源序列,为最终克隆黄花苜蓿MfCAS15-1抗寒基因全长奠定了基础。
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| 关 键 词： | 黄花苜蓿 冷诱导基因 克隆 序列分析 |
Cloning and Sequence Analysis of Cold Resistance Gene from Wild Medicago falcata |
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| Liu Yaling,Wang Junjie,Yun Jinfeng,Zhao Yan,Shi Fengmin.Cloning and Sequence Analysis of Cold Resistance Gene from Wild Medicago falcata[J].Biotechnology Bulletin,2010(4). |
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| Authors: | Liu Yaling Wang Junjie Yun Jinfeng Zhao Yan Shi Fengmin |
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| Institution: | Liu Yaling1,2Wang Junjie1,2Yun Jinfeng1,2Zhao Yan1,3Shi Fengmin1,2(1College of Ecology , Environmental Science,Inner Mongolia Agricultural University,Huhhot 010019,2Inner Mongolia Engineering , Technique Center for Forage Breeding , Reproduction,3 Inner Mongolia Academy of Agriculture , Animal Husb,ry Sciences,Huhhot 010031) |
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| Abstract: | According to cDNA sequence of cas15B (Accession number:L12462) of Medicago sativa,a pair of specific primers were designed,by which a new cDNA fragment of 550 bp was amplified by RT-PCR from total RNA of Medicago falcate that suffered from cold stress.Sequencing result and sequence analysis showed that this amplified fragment encoded 159 amino acids,four of which were Gly,Glu,His and Lys,accounting for 70% predicted.The polypeptide contains a decapeptide motif repeated five times at regular intervals with t... |
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| Keywords: | Medicago falcate Cold resistance gene Clone Sequence analysis |
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